MGRA_STAA8
ID MGRA_STAA8 Reviewed; 147 AA.
AC Q2G0B1; Q7X448; Q83ZI4;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=HTH-type transcriptional regulator MgrA;
DE AltName: Full=Regulator of autolytic activity;
GN Name=mgrA; Synonyms=norR, rat; OrderedLocusNames=SAOUHSC_00694;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15, AND FUNCTION.
RX PubMed=12730173; DOI=10.1128/jb.185.10.3127-3138.2003;
RA Truong-Bolduc Q.C., Zhang X., Hooper D.C.;
RT "Characterization of NorR protein, a multifunctional regulator of norA
RT expression in Staphylococcus aureus.";
RL J. Bacteriol. 185:3127-3138(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [3]
RP FUNCTION, AND REGULATION.
RX PubMed=12791130; DOI=10.1046/j.1365-2958.2003.03503.x;
RA Ingavale S.S., Van Wamel W., Cheung A.L.;
RT "Characterization of RAT, an autolysis regulator in Staphylococcus
RT aureus.";
RL Mol. Microbiol. 48:1451-1466(2003).
RN [4]
RP FUNCTION.
RX PubMed=15292128; DOI=10.1128/jb.186.16.5267-5280.2004;
RA Manna A.C., Ingavale S.S., Maloney M., van Wamel W., Cheung A.L.;
RT "Identification of sarV (SA2062), a new transcriptional regulator, is
RT repressed by SarA and MgrA (SA0641) and involved in the regulation of
RT autolysis in Staphylococcus aureus.";
RL J. Bacteriol. 186:5267-5280(2004).
RN [5]
RP FUNCTION.
RX PubMed=15731040; DOI=10.1128/iai.73.3.1423-1431.2005;
RA Ingavale S.S., van Wamel W., Luong T.T., Lee C.Y., Cheung A.L.;
RT "Rat/MgrA, a regulator of autolysis, is a regulator of virulence genes in
RT Staphylococcus aureus.";
RL Infect. Immun. 73:1423-1431(2005).
RN [6]
RP FUNCTION.
RX PubMed=15774883; DOI=10.1128/jb.187.7.2395-2405.2005;
RA Truong-Bolduc Q.C., Dunman P.M., Strahilevitz J., Projan S.J., Hooper D.C.;
RT "MgrA is a multiple regulator of two new efflux pumps in Staphylococcus
RT aureus.";
RL J. Bacteriol. 187:2395-2405(2005).
CC -!- FUNCTION: Regulatory protein involved in autolytic activity, multidrug
CC resistance and virulence. Controls autolysis by inactivating LytM, LytN
CC (autolysins) and SarV (autolysis activator) and activating ArlRS, LrgAB
CC and LytSR (autolysis inhibitors). Acts as a dual regulator for
CC resistance to multiple drugs by inactivating NorB and tet38 and
CC activating NorA. Positively controls the expression of virulence
CC accessory gene regulator (agr) to promote alpha-hemolysin (hla)
CC transcription and down-regulates staphylococcal accessory regulator
CC (sarS), leading to repression of surface protein A (spa). Binds
CC directly to hla promoter to augment its activation. Binds to sarS
CC promoter to down-regulate spa expression. {ECO:0000269|PubMed:12730173,
CC ECO:0000269|PubMed:12791130, ECO:0000269|PubMed:15292128,
CC ECO:0000269|PubMed:15731040, ECO:0000269|PubMed:15774883}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Autoregulated.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY266422; AAP24056.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD29827.1; -; Genomic_DNA.
DR RefSeq; WP_001283444.1; NZ_LS483365.1.
DR RefSeq; YP_499253.1; NC_007795.1.
DR AlphaFoldDB; Q2G0B1; -.
DR SMR; Q2G0B1; -.
DR STRING; 1280.SAXN108_0755; -.
DR EnsemblBacteria; ABD29827; ABD29827; SAOUHSC_00694.
DR GeneID; 3920998; -.
DR GeneID; 66838978; -.
DR KEGG; sao:SAOUHSC_00694; -.
DR PATRIC; fig|93061.5.peg.624; -.
DR eggNOG; COG1846; Bacteria.
DR HOGENOM; CLU_083287_3_2_9; -.
DR OMA; AHRMTKS; -.
DR PRO; PR:Q2G0B1; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000835; HTH_MarR-typ.
DR InterPro; IPR023187; Tscrpt_reg_MarR-type_CS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF01047; MarR; 1.
DR SMART; SM00347; HTH_MARR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS01117; HTH_MARR_1; 1.
DR PROSITE; PS50995; HTH_MARR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW Reference proteome; Repressor; Transcription; Transcription regulation;
KW Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:12730173"
FT CHAIN 2..147
FT /note="HTH-type transcriptional regulator MgrA"
FT /id="PRO_0000247940"
FT DOMAIN 8..139
FT /note="HTH marR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT DNA_BIND 55..78
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
SQ SEQUENCE 147 AA; 17089 MW; F5EA62A451EF9FC8 CRC64;
MSDQHNLKEQ LCFSLYNAQR QVNRYYSNKV FKKYNLTYPQ FLVLTILWDE SPVNVKKVVT
ELALDTGTVS PLLKRMEQVD LIKRERSEVD QREVFIHLTD KSETIRPELS NASDKVASAS
SLSQDEVKEL NRLLGKVIHA FDETKEK
