ID   MGRA_STAAU              Reviewed;         147 AA.
AC   P0C1S0; Q7X448; Q83ZI4;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=HTH-type transcriptional regulator MgrA;
DE   AltName: Full=Regulator of autolytic activity;
GN   Name=mgrA; Synonyms=mgr;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=Becker;
RX   PubMed=12813062; DOI=10.1128/jb.185.13.3703-3710.2003;
RA   Luong T.T., Newell S.W., Lee C.Y.;
RT   "Mgr, a novel global regulator in Staphylococcus aureus.";
RL   J. Bacteriol. 185:3703-3710(2003).
CC   -!- FUNCTION: Regulatory protein involved in autolytic activity, multidrug
CC       resistance and virulence (By similarity). Negatively controls spa
CC       (surface protein A) transcription. Activates type 8 capsular
CC       polysaccharide (cap8) and nuclease (nuc) biosynthesis. Represses
CC       coagulase and protease production. {ECO:0000250,
CC       ECO:0000269|PubMed:12813062}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- MISCELLANEOUS: In strain Becker, gene disruption may lead to increase
CC       in alpha-hemolysin (hla).
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DR   EMBL; AY293568; AAP48794.1; -; Genomic_DNA.
DR   RefSeq; WP_001283444.1; NZ_WYDB01000004.1.
DR   PDB; 2BV6; X-ray; 2.80 A; A=6-143.
DR   PDBsum; 2BV6; -.
DR   AlphaFoldDB; P0C1S0; -.
DR   SMR; P0C1S0; -.
DR   BindingDB; P0C1S0; -.
DR   ChEMBL; CHEMBL2331056; -.
DR   GeneID; 66838978; -.
DR   OMA; AHRMTKS; -.
DR   EvolutionaryTrace; P0C1S0; -.
DR   PHI-base; PHI:3058; -.
DR   PHI-base; PHI:6255; -.
DR   PRO; PR:P0C1S0; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR023187; Tscrpt_reg_MarR-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS01117; HTH_MARR_1; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation; Virulence.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..147
FT                   /note="HTH-type transcriptional regulator MgrA"
FT                   /id="PRO_0000054366"
FT   DOMAIN          8..139
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   DNA_BIND        55..78
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   HELIX           11..29
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           31..34
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           38..49
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   STRAND          50..54
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           55..61
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   TURN            66..68
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           69..78
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   STRAND          81..86
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   STRAND          88..90
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   STRAND          94..98
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           100..109
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           112..119
FT                   /evidence="ECO:0007829|PDB:2BV6"
FT   HELIX           124..138
FT                   /evidence="ECO:0007829|PDB:2BV6"
SQ   SEQUENCE   147 AA;  17089 MW;  F5EA62A451EF9FC8 CRC64;
     MSDQHNLKEQ LCFSLYNAQR QVNRYYSNKV FKKYNLTYPQ FLVLTILWDE SPVNVKKVVT
     ELALDTGTVS PLLKRMEQVD LIKRERSEVD QREVFIHLTD KSETIRPELS NASDKVASAS
     SLSQDEVKEL NRLLGKVIHA FDETKEK