ID   MGTA_MYCTU              Reviewed;         378 AA.
AC   P9WMY5; L0T409; O06423; Q7BTG3; Q8VKJ0;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=GDP-mannose-dependent alpha-mannosyltransferase;
DE            EC=2.4.1.-;
DE   AltName: Full=Guanosine diphosphomannose-dependent alpha-mannosyltransferase;
GN   Name=mgtA; Synonyms=pimB; OrderedLocusNames=Rv0557;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=10531370; DOI=10.1074/jbc.274.44.31625;
RA   Schaeffer M.L., Khoo K.H., Besra G.S., Chatterjee D., Brennan P.J.,
RA   Belisle J.T., Inamine J.M.;
RT   "The pimB gene of Mycobacterium tuberculosis encodes a mannosyltransferase
RT   involved in lipoarabinomannan biosynthesis.";
RL   J. Biol. Chem. 274:31625-31631(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [4]
RP   FUNCTION IN MANGLCAGROAC2 BIOSYNTHESIS, AND NOMENCLATURE.
RX   PubMed=19395496; DOI=10.1128/jb.01729-08;
RA   Mishra A.K., Batt S., Krumbach K., Eggeling L., Besra G.S.;
RT   "Characterization of the Corynebacterium glutamicum deltapimB' deltamgtA
RT   double deletion mutant and the role of Mycobacterium tuberculosis
RT   orthologues Rv2188c and Rv0557 in glycolipid biosynthesis.";
RL   J. Bacteriol. 191:4465-4472(2009).
CC   -!- FUNCTION: Catalyzes the addition of a mannose residue from GDP-D-
CC       mannose to GlcAGroAc2 to generate 1,2-di-O-C16/C18:1-(alpha-D-
CC       mannopyranosyl)-(1-4)-(alpha-D-glucopyranosyluronic acid)-(1-3)-
CC       glycerol(ManGlcAGroAc2). {ECO:0000269|PubMed:19395496}.
CC   -!- PATHWAY: Phospholipid metabolism; phosphatidylinositol metabolism.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR   EMBL; AF061562; AAC63250.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP43295.1; -; Genomic_DNA.
DR   PIR; H70548; H70548.
DR   RefSeq; NP_215071.1; NC_000962.3.
DR   RefSeq; WP_003900963.1; NC_000962.3.
DR   AlphaFoldDB; P9WMY5; -.
DR   SMR; P9WMY5; -.
DR   STRING; 83332.Rv0557; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   PaxDb; P9WMY5; -.
DR   DNASU; 887609; -.
DR   GeneID; 887609; -.
DR   KEGG; mtu:Rv0557; -.
DR   PATRIC; fig|83332.111.peg.614; -.
DR   TubercuList; Rv0557; -.
DR   eggNOG; COG0438; Bacteria.
DR   OMA; ERVDLWQ; -.
DR   PhylomeDB; P9WMY5; -.
DR   BioCyc; MetaCyc:G185E-6398-MON; -.
DR   BRENDA; 2.4.1.346; 3445.
DR   BRENDA; 2.4.1.B74; 3445.
DR   UniPathway; UPA00949; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0000030; F:mannosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0046488; P:phosphatidylinositol metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Lipid biosynthesis; Lipid metabolism;
KW   Phospholipid biosynthesis; Phospholipid metabolism; Reference proteome;
KW   Transferase; Virulence.
FT   CHAIN           1..378
FT                   /note="GDP-mannose-dependent alpha-mannosyltransferase"
FT                   /id="PRO_0000393729"
SQ   SEQUENCE   378 AA;  41238 MW;  E1E5524A88A52D65 CRC64;
     MCGVRVAIVA ESFLPQVNGV SNSVVKVLEH LRRTGHEALV IAPDTPPGED RAERLHDGVR
     VHRVPSRMFP KVTTLPLGVP TFRMLRALRG FDPDVVHLAS PALLGYGGLH AARRLGVPTV
     AVYQTDVPGF ASSYGIPMTA RAAWAWFRHL HRLADRTLAP STATMESLIA QGIPRVHRWA
     RGVDVQRFAP SARNEVLRRR WSPDGKPIVG FVGRLAPEKH VDRLTGLAAS GAVRLVIVGD
     GIDRARLQSA MPTAVFTGAR YGKELAEAYA SMDVFVHSGE HETFCQVVQE ALASGLPVIA
     PDAGGPRDLI TPHRTGLLLP VGEFEHRLPD AVAHLVHERQ RYALAARRSV LGRSWPVVCD
     ELLGHYEAVR GRRTTQAA