MHO1_YEAST
ID MHO1_YEAST Reviewed; 338 AA.
AC P47085; D6VWI3;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=MEMO1 family protein MHO1 {ECO:0000303|PubMed:22412880};
DE AltName: Full=Memo-homolog 1 {ECO:0000303|PubMed:22412880};
GN Name=MHO1 {ECO:0000303|PubMed:22412880}; OrderedLocusNames=YJR008W;
GN ORFNames=J1431, YJR83.9;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INDUCTION.
RX PubMed=11102521; DOI=10.1091/mbc.11.12.4241;
RA Gasch A.P., Spellman P.T., Kao C.M., Carmel-Harel O., Eisen M.B., Storz G.,
RA Botstein D., Brown P.O.;
RT "Genomic expression programs in the response of yeast cells to
RT environmental changes.";
RL Mol. Biol. Cell 11:4241-4257(2000).
RN [4]
RP INDUCTION.
RX PubMed=14561723; DOI=10.1093/jb/mvg155;
RA Sakaki K., Tashiro K., Kuhara S., Mihara K.;
RT "Response of genes associated with mitochondrial function to mild heat
RT stress in yeast Saccharomyces cerevisiae.";
RL J. Biochem. 134:373-384(2003).
RN [5]
RP INDUCTION.
RX PubMed=12871953; DOI=10.1074/jbc.m303008200;
RA Santiago T.C., Mamoun C.B.;
RT "Genome expression analysis in yeast reveals novel transcriptional
RT regulation by inositol and choline and new regulatory functions for Opi1p,
RT Ino2p, and Ino4p.";
RL J. Biol. Chem. 278:38723-38730(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=22412880; DOI=10.1371/journal.pone.0032501;
RA Schlatter I.D., Meira M., Ueberschlag V., Hoepfner D., Movva R.,
RA Hynes N.E.;
RT "MHO1, an evolutionarily conserved gene, is synthetic lethal with PLC1;
RT Mho1p has a role in invasive growth.";
RL PLoS ONE 7:E32501-E32501(2012).
CC -!- FUNCTION: Plays a role in haploid invasive growth under conditions of
CC nutrient insufficiency, suggesting that the function of the MEMO1
CC family in cell motility/invasion is conserved across species.
CC {ECO:0000269|PubMed:22412880}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22412880}. Nucleus
CC {ECO:0000269|PubMed:22412880}. Note=Is actively exported from the
CC nucleus. {ECO:0000269|PubMed:22412880}.
CC -!- INDUCTION: Expression is induced by mild heat-stress on a non-
CC fermentable carbon source (PubMed:14561723). Expression is also induced
CC upon entry into stationary phase and upon nitrogen deprivation
CC (PubMed:11102521). Expression is repressed by inosine and choline in an
CC OPI1-dependent manner (PubMed:12871953). {ECO:0000269|PubMed:11102521,
CC ECO:0000269|PubMed:12871953, ECO:0000269|PubMed:14561723}.
CC -!- DISRUPTION PHENOTYPE: Is synthetic lethal with PLC1.
CC {ECO:0000269|PubMed:22412880}.
CC -!- MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the MEMO1 family. {ECO:0000305}.
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DR EMBL; X87611; CAA60930.1; -; Genomic_DNA.
DR EMBL; Z49508; CAA89530.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08799.1; -; Genomic_DNA.
DR PIR; S55196; S55196.
DR RefSeq; NP_012541.1; NM_001181665.1.
DR AlphaFoldDB; P47085; -.
DR SMR; P47085; -.
DR BioGRID; 33764; 30.
DR DIP; DIP-5693N; -.
DR IntAct; P47085; 1.
DR STRING; 4932.YJR008W; -.
DR iPTMnet; P47085; -.
DR MaxQB; P47085; -.
DR PaxDb; P47085; -.
DR PRIDE; P47085; -.
DR EnsemblFungi; YJR008W_mRNA; YJR008W; YJR008W.
DR GeneID; 853464; -.
DR KEGG; sce:YJR008W; -.
DR SGD; S000003768; MHO1.
DR VEuPathDB; FungiDB:YJR008W; -.
DR eggNOG; KOG3086; Eukaryota.
DR GeneTree; ENSGT00390000006408; -.
DR HOGENOM; CLU_038085_0_0_1; -.
DR InParanoid; P47085; -.
DR OMA; IMVGNTS; -.
DR BioCyc; YEAST:G3O-31654-MON; -.
DR PRO; PR:P47085; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47085; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR CDD; cd07361; MEMO_like; 1.
DR HAMAP; MF_00055; MEMO1; 1.
DR InterPro; IPR002737; MEMO1_fam.
DR PANTHER; PTHR11060; PTHR11060; 1.
DR Pfam; PF01875; Memo; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..338
FT /note="MEMO1 family protein MHO1"
FT /id="PRO_0000134398"
SQ SEQUENCE 338 AA; 38482 MW; 6C0726E2EFD40ECE CRC64;
MSIRPATHAG SWYSNRAQEL SQQLHTYLIK STLKGPIHNA RIIICPHAGY RYCGPTMAYS
YASLDLNRNV KRIFILGPSH HIYFKNQILV SAFSELETPL GNLKVDTDLC KTLIQKEYPE
NGKKLFKPMD HDTDMAEHSL EMQLPMLVET LKWREISLDT VKVFPMMVSH NSVDVDRCIG
NILSEYIKDP NNLFIVSSDF CHWGRRFQYT GYVGSKEELN DAIQEETEVE MLTARSKLSH
HQVPIWQSIE IMDRYAMKTL SDTPNGERYD AWKQYLEITG NTICGEKPIS VILSALSKIR
DAGPSGIKFQ WPNYSQSSHV TSIDDSSVSY ASGYVTIG
