MHPA_MYCS2
ID MHPA_MYCS2 Reviewed; 562 AA.
AC A0R1T4; I7FIJ0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase {ECO:0000255|HAMAP-Rule:MF_01652};
DE Short=3-HCI hydroxylase {ECO:0000255|HAMAP-Rule:MF_01652};
DE Short=3-HPP hydroxylase {ECO:0000255|HAMAP-Rule:MF_01652};
DE EC=1.14.13.127 {ECO:0000255|HAMAP-Rule:MF_01652};
DE AltName: Full=Flavin-type monooxygenase;
GN Name=mhpA {ECO:0000255|HAMAP-Rule:MF_01652};
GN OrderedLocusNames=MSMEG_4866, MSMEI_4741;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: Catalyzes the insertion of one atom of molecular oxygen into
CC position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP)
CC and hydroxycinnamic acid (3HCI). {ECO:0000255|HAMAP-Rule:MF_01652}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(3-hydroxyphenyl)propanoate + H(+) + NADH + O2 = 3-(2,3-
CC dihydroxyphenyl)propanoate + H2O + NAD(+); Xref=Rhea:RHEA:24785,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:46951, ChEBI:CHEBI:57277, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.14.13.127; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01652};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-3-(3-hydroxyphenyl)prop-2-enoate + H(+) + NADH + O2 =
CC (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD(+);
CC Xref=Rhea:RHEA:27846, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:47928, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58642; EC=1.14.13.127;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01652};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01652};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000255|HAMAP-Rule:MF_01652}.
CC -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC {ECO:0000255|HAMAP-Rule:MF_01652}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK72149.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK72149.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001663; AFP41190.1; -; Genomic_DNA.
DR RefSeq; WP_014878169.1; NZ_SIJM01000024.1.
DR RefSeq; YP_889122.1; NC_008596.1.
DR AlphaFoldDB; A0R1T4; -.
DR SMR; A0R1T4; -.
DR STRING; 246196.MSMEI_4741; -.
DR EnsemblBacteria; ABK72149; ABK72149; MSMEG_4866.
DR EnsemblBacteria; AFP41190; AFP41190; MSMEI_4741.
DR GeneID; 66736171; -.
DR KEGG; msg:MSMEI_4741; -.
DR KEGG; msm:MSMEG_4866; -.
DR PATRIC; fig|246196.19.peg.4748; -.
DR eggNOG; COG0654; Bacteria.
DR OMA; RRVYTHH; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0008688; F:3-(3-hydroxyphenyl)propionate hydroxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0019622; P:3-(3-hydroxy)phenylpropionate catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.50.50.60; -; 1.
DR HAMAP; MF_01652; MhpA; 1.
DR InterPro; IPR023786; 3-HPP/3HCI_hydroxylase.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; FAD; Flavoprotein; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..562
FT /note="3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic
FT acid hydroxylase"
FT /id="PRO_0000337637"
FT BINDING 8..37
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01652"
FT BINDING 275..285
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01652"
SQ SEQUENCE 562 AA; 61387 MW; 1948C011F7622882 CRC64;
MNSPGAVDVV IVGAGPVGLT LANILGGQGV RTLIIEERET LIDYPRGVGL DDESLRTFQS
IGLVDAILPH TVPNQILRFY DANRRLLAEM APPDARFGWP KRNGFVQPMV DAELLAGLDR
FDHVEVMWGR RMQTIAEDAD GVTVEVSGPD GPASVHAQYV VGCDGGRSAT RHLMGVSFDG
TTSSTRWVVI DLANDPLGHP NSEVGADPQR PYASISIAHG IRRFEFMIHA DETDEQAEDP
EFVAELLRPF VPHPDRVDVI RRRVYTHHSR IAGSFRKGRM LLAGDAAHLM PVWQGQGYNS
GIRDAFNLGW KLAAVVRGQA GDALLDTYDA ERRKHARAMI DLSTMVGRVI SPTNRKVAAL
RDKLIRGASI VPTLKRYVLE MRFKPMPRYH EGAVYHAKPP TPASPVGTLF IQPRVDTREQ
DNVLLDDVLG TGFAVLCWNN NPRTLLGEAA FTRWKALGAT FIAARPSTQL HWTKDDDPDV
VIVGDRTGAL KAFFDAHTES VLVLRPDRCI AGADIAQRAP ELSTALFGIL HLRQGGENGA
TGPVLYVPQP TAESSGTVGR AS
