ID   MHPD1_PSEPU             Reviewed;         265 AA.
AC   Q400K2;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=2-keto-4-pentenoate hydratase 1 {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase 1 {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD1 {ECO:0000255|HAMAP-Rule:MF_01655}; Synonyms=orcD;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ORC;
RA   Straganz G.D., Glieder A., Steiner W.;
RT   "Cloning of genes involved in meta-cleavage pathways of Pseudomonas putida
RT   orc: nucleotide sequences of genes, characterization of two dioxygenases
RT   and identification of the orcinol pathway.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; AF534914; AAQ10536.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q400K2; -.
DR   SMR; Q400K2; -.
DR   PRIDE; Q400K2; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase.
FT   CHAIN           1..265
FT                   /note="2-keto-4-pentenoate hydratase 1"
FT                   /id="PRO_0000337797"
SQ   SEQUENCE   265 AA;  28291 MW;  D27BA5A9A9CA8466 CRC64;
     MTISQETLER LAADLRRAEQ QGEAIPPLRD AIGAENGEAA YAIQRLNVAH ALAAGRRLVG
     RKVGLTNPKV QAQLGVDQPD FGCLFADMAY GDNETVPFDR VLQPKIEREI ALVLERDLPA
     RTPTWPNVAN RHRLGACRPL EIVGTVSPTW NIRFADTVAD NASSGLFVLG GPARRLDGLD
     LRGAAMRMTR NDELVSSGSG AECLGHPLNA AVWLARTLAR LGDPLKAGDI VLTGALGPMV
     AVNAGDRFDA EIDGLGRVGV TFSQS