ID   MHPD2_DECAR             Reviewed;         262 AA.
AC   Q47GC8;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=2-keto-4-pentenoate hydratase 2 {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase 2 {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD2 {ECO:0000255|HAMAP-Rule:MF_01655}; OrderedLocusNames=Daro_1354;
OS   Dechloromonas aromatica (strain RCB).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Azonexaceae;
OC   Dechloromonas.
OX   NCBI_TaxID=159087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RCB;
RX   PubMed=19650930; DOI=10.1186/1471-2164-10-351;
RA   Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G.,
RA   Lapidus A.;
RT   "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB:
RT   indications of a surprisingly complex life-style and cryptic anaerobic
RT   pathways for aromatic degradation.";
RL   BMC Genomics 10:351-351(2009).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; CP000089; AAZ46103.1; -; Genomic_DNA.
DR   RefSeq; WP_011287112.1; NC_007298.1.
DR   AlphaFoldDB; Q47GC8; -.
DR   SMR; Q47GC8; -.
DR   STRING; 159087.Daro_1354; -.
DR   EnsemblBacteria; AAZ46103; AAZ46103; Daro_1354.
DR   KEGG; dar:Daro_1354; -.
DR   eggNOG; COG3971; Bacteria.
DR   HOGENOM; CLU_060136_4_1_4; -.
DR   OMA; RIVGHKI; -.
DR   OrthoDB; 1314120at2; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase.
FT   CHAIN           1..262
FT                   /note="2-keto-4-pentenoate hydratase 2"
FT                   /id="PRO_0000337792"
SQ   SEQUENCE   262 AA;  27866 MW;  6F5F2E2AA1427DDC CRC64;
     MTNNFMAPEA AAALLKAATA DGRPIAPLRD RLAIADQDSA YAVQEINTRA WLAEGRRLVG
     RKIGLTSLAV QAQLGVDQPD FGMLFADMAV GDGEIVAAGR LIQPKVEAEV ALILGRDLTQ
     ERHTYADLIR ATEYALPSIE IVDSRIENWN IKFVDTVADN ASSGLFVLGG RSVRLCDIDL
     TACAMEMKRG DEVVSRGNGR ACLGSPLNAA IWLADVMVRC GRPLQAGDIV LTGALGPMVA
     VKSGERFDVS IEGLGNVSAL FA