ID   MHPD2_PSEPU             Reviewed;         270 AA.
AC   Q49KF9;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=2-keto-4-pentenoate hydratase 2 {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase 2 {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD2 {ECO:0000255|HAMAP-Rule:MF_01655}; Synonyms=cbzJ2;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid pKW1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=GJ31;
RA   Reineke W., Kunze M.;
RT   "GJ31 meta-operon.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; AY831461; AAX50132.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q49KF9; -.
DR   SMR; Q49KF9; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Plasmid.
FT   CHAIN           1..270
FT                   /note="2-keto-4-pentenoate hydratase 2"
FT                   /id="PRO_0000337798"
SQ   SEQUENCE   270 AA;  28504 MW;  3107EB3334D3C0ED CRC64;
     MNTSQETLER LAAELRKAEG KGEPVGPLRD VIGATNVEHA YAIQRLNQAY AIANGRRLVG
     RKIGLTNPKV QAQLGVDQPD FGCLFADMAY GDNEVIPFER VLQPKIEAEI ALILERDLPS
     ASTTFADVIA ATGWVVPALE VVGSRIEGWN IKFADTVADN ASSGCFVLGG PARRIDGLDL
     RGATMRMSKN GEVVSSGSGG ECLGNPLNAA VWLARTMAEF GEPLKAGDII LTGALGPMVG
     VSAGDHFEAE IEGLGRVGVS FSVDVTEVLK