ID   MHPD_BURVG              Reviewed;         262 AA.
AC   A4JPX9;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655};
GN   OrderedLocusNames=Bcep1808_5386;
OS   Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia
OS   (strain R1808)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=269482;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G4 / LMG 22486;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 2 of Burkholderia vietnamiensis G4.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; CP000615; ABO58332.1; -; Genomic_DNA.
DR   AlphaFoldDB; A4JPX9; -.
DR   SMR; A4JPX9; -.
DR   STRING; 269482.Bcep1808_5386; -.
DR   EnsemblBacteria; ABO58332; ABO58332; Bcep1808_5386.
DR   KEGG; bvi:Bcep1808_5386; -.
DR   eggNOG; COG3971; Bacteria.
DR   HOGENOM; CLU_060136_4_1_4; -.
DR   OMA; IRDWSIG; -.
DR   UniPathway; UPA00714; -.
DR   Proteomes; UP000002287; Chromosome 2.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Reference proteome.
FT   CHAIN           1..262
FT                   /note="2-keto-4-pentenoate hydratase"
FT                   /id="PRO_0000337788"
SQ   SEQUENCE   262 AA;  27541 MW;  C291F16304735939 CRC64;
     MSDDNSIASM AARLREAQAS GRTIAPLRDT CPGGDATLAY AIQQINNDLR KTNGERVVGR
     KIGLTSPAVQ KQLGVDQPDF GALFASMAYG DGEPMPLASL IQPKVEAEIA LVLERDLTAE
     KHTFADLISA TAYAVAAIEV VDSRIRDWDI RFFDTVADNA SSALFVLGSR PVLLRDIDLT
     ACAMTLAQDG EVLSRGNGAA CLGNPLNAAA WLADRMVRLG TPLRAGDVVL TGALGPMVAV
     KAAGTYAAHI DGLGSVRATF TE