ID   MHPD_COMTE              Reviewed;         260 AA.
AC   Q9S156;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655};
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN CATABOLISM OF
RP   PHENYLPROPIONIC AND CINNAMIC ACIDS.
RC   STRAIN=TA441;
RX   PubMed=10537203; DOI=10.1099/00221287-145-10-2813;
RA   Arai H., Yamamoto T., Ohishi T., Shimizu T., Nakata T., Kudo T.;
RT   "Genetic organization and characteristics of the 3-(3-
RT   hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni
RT   TA441.";
RL   Microbiology 145:2813-2820(1999).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655, ECO:0000269|PubMed:10537203}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA82880.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB024335; BAA82880.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q9S156; -.
DR   SMR; Q9S156; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Lyase.
FT   CHAIN           1..260
FT                   /note="2-keto-4-pentenoate hydratase"
FT                   /id="PRO_0000337790"
SQ   SEQUENCE   260 AA;  27559 MW;  1E9B2990B1ADDBA2 CRC64;
     MPTTTQIQAW AERLRHAEAT ATPIAPLREE ITDNDSAYAV QLVNVQYAQS QGRRIVGRKI
     GLTSLAVQKQ LGVDQPDFGT LFADMLYGDD EAVPLSRTLQ PKVEAEVALV LAKDLERPDT
     TLVDVISATA YVLPAIEIVG SRIADWNIRF IDTVADNASS GLVVLGAVPT ALNALDLKLC
     QMQMTRNGDV VSTGSGGACL GHPLNAAVWL ARRLANLGQP LRAGDLVLTG ALGPMVAVNA
     GDRFEARISG IGSVCAQFEG