ID   MHPD_KLEP7              Reviewed;         268 AA.
AC   A6TAC6;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655};
GN   OrderedLocusNames=KPN78578_20860; ORFNames=KPN_02119;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABR77547.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000647; ABR77547.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_002908598.1; NC_009648.1.
DR   AlphaFoldDB; A6TAC6; -.
DR   SMR; A6TAC6; -.
DR   STRING; 272620.KPN_02119; -.
DR   EnsemblBacteria; ABR77547; ABR77547; KPN_02119.
DR   KEGG; kpn:KPN_02119; -.
DR   HOGENOM; CLU_060136_4_1_6; -.
DR   OMA; IRDWSIG; -.
DR   UniPathway; UPA00714; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Reference proteome.
FT   CHAIN           1..268
FT                   /note="2-keto-4-pentenoate hydratase"
FT                   /id="PRO_0000337796"
SQ   SEQUENCE   268 AA;  28678 MW;  21F0E569B2240615 CRC64;
     MTFSLDALAR QLRDAEQSGQ AIAPLRDILG VDNADAAYAI QRLNVQHHVA HGRRVVGRKV
     GLTHPKVQQQ LGVNQPDFGT LFADMCYGDN AEVPFGRVLQ PKVEAEIALV LKQDLPHADT
     TFDELYNAIE WVLPALEVVG SRIRDWSIGF VDTVADNASC GLYVIGGPAQ RPAGLDLKQC
     AMHMTRNQEL VSSGRGSECL GHPLNAAVWL ARKLASLGEP LRAGDIVLTG ALGPMVTINE
     GDSFVAHIEG IGSVAARFVA AGEGDRDA