ID   MHPD_PARXL              Reviewed;         269 AA.
AC   Q13VU0;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655}; OrderedLocusNames=Bxeno_A3261;
GN   ORFNames=Bxe_A1149;
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400;
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V.,
RA   Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J.,
RA   Parnell J.J., Ramette A., Richardson P., Seeger M., Smith D., Spilker T.,
RA   Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome
RT   shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC         dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
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DR   EMBL; CP000270; ABE31799.1; -; Genomic_DNA.
DR   RefSeq; WP_011489336.1; NZ_CP008760.1.
DR   AlphaFoldDB; Q13VU0; -.
DR   SMR; Q13VU0; -.
DR   STRING; 266265.Bxe_A1149; -.
DR   EnsemblBacteria; ABE31799; ABE31799; Bxe_A1149.
DR   KEGG; bxb:DR64_3308; -.
DR   KEGG; bxe:Bxe_A1149; -.
DR   eggNOG; COG3971; Bacteria.
DR   OMA; PLYEADM; -.
DR   OrthoDB; 1314120at2; -.
DR   SABIO-RK; Q13VU0; -.
DR   UniPathway; UPA00714; -.
DR   Proteomes; UP000001817; Chromosome 1.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Reference proteome.
FT   CHAIN           1..269
FT                   /note="2-keto-4-pentenoate hydratase"
FT                   /id="PRO_0000337789"
SQ   SEQUENCE   269 AA;  28759 MW;  9A9B4B8A3B95CFF0 CRC64;
     MTPQQREEAA QSLYQAMQSG KPIAPLRDTF PDMNVDDAYA IQSINTQRRI SLGRRVVGRK
     IGLTSVVVQQ QLGVDEPDFG ALFDDMSFGD AETIPLSILH QPKVEAEIGF VLGRDLDTEQ
     PTHQEVLQAV DYVVPALEIV GSRIADWNIK FVDTVADNAS SGVYVLGSTP ISPRGLDLSL
     VGMCLSRRGE PVSTGAGAAC LGTPLNAVVW LARTMSRLGK PLRAGELILS GALGPMVAVK
     PGDVFECHIN GVGSVRTEFE SNQMNGVAA