MI25_ARATH
ID MI25_ARATH Reviewed; 192 AA.
AC Q04613; A7KNI5;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=ATP synthase protein MI25;
DE AltName: Full=ORF25;
GN OrderedLocusNames=AtMg00640;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1494348; DOI=10.1007/bf00279640;
RA Brandt P., Sunkel S., Unseld M., Brennicke A., Knoop V.;
RT "The nad4L gene is encoded between exon c of nad5 and orf25 in the
RT Arabidopsis mitochondrial genome.";
RL Mol. Gen. Genet. 236:33-38(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8988169; DOI=10.1038/ng0197-57;
RA Unseld M., Marienfeld J.R., Brandt P., Brennicke A.;
RT "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in
RT 366,924 nucleotides.";
RL Nat. Genet. 15:57-61(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND RNA EDITING.
RX PubMed=10611383; DOI=10.1073/pnas.96.26.15324;
RA Giege P., Brennicke A.;
RT "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in
RT ORFs.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 15-182, AND RNA EDITING.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Rosette leaf;
RX PubMed=17565941; DOI=10.1534/genetics.107.073585;
RA Bentolila S., Elliott L.E., Hanson M.R.;
RT "Genetic architecture of mitochondrial editing in Arabidopsis thaliana.";
RL Genetics 178:1693-1708(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=12681508; DOI=10.1016/s0014-5793(03)00264-3;
RA Heazlewood J.L., Whelan J., Millar A.H.;
RT "The products of the mitochondrial orf25 and orfB genes are F(0) components
RT in the plant F(1)F(0) ATP synthase.";
RL FEBS Lett. 540:201-205(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
CC -!- FUNCTION: This is one of the chains of the nonenzymatic component
CC (CF(0) subunit) of the mitochondrial ATPase complex.
CC -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC - and CF(0) - the membrane proton channel. CF(1) has five subunits:
CC alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main
CC subunits: a, b and c (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:14671022}; Single-pass membrane protein
CC {ECO:0000269|PubMed:14671022}.
CC -!- RNA EDITING: Modified_positions=30 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 72 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 83 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 84 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 132 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 139 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941};
CC -!- SIMILARITY: Belongs to the ATPase protein MI25 family. {ECO:0000305}.
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DR EMBL; X67105; CAA47480.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Y08501; CAA69748.3; ALT_SEQ; Genomic_DNA.
DR EMBL; EF488928; ABS50640.1; -; mRNA.
DR EMBL; EF488929; ABS50641.1; -; mRNA.
DR PIR; S34401; S34401.
DR RefSeq; NP_085524.1; NC_001284.2.
DR AlphaFoldDB; Q04613; -.
DR SMR; Q04613; -.
DR IntAct; Q04613; 2.
DR STRING; 3702.ATMG00640.1; -.
DR PaxDb; Q04613; -.
DR PeptideAtlas; Q04613; -.
DR PRIDE; Q04613; -.
DR ProteomicsDB; 250849; -.
DR Araport; ATMG00640; -.
DR eggNOG; ENOG502RFCF; Eukaryota.
DR HOGENOM; CLU_123596_0_0_1; -.
DR InParanoid; Q04613; -.
DR OrthoDB; 1299259at2759; -.
DR PRO; PR:Q04613; -.
DR Proteomes; UP000006548; Mitochondrion (cv. C24).
DR ExpressionAtlas; Q04613; baseline and differential.
DR Genevisible; Q04613; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000276; C:mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); IBA:GO_Central.
DR GO; GO:0015078; F:proton transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015986; P:proton motive force-driven ATP synthesis; IEA:InterPro.
DR InterPro; IPR008688; ATP_synth_Bsub_B/MI25.
DR InterPro; IPR044988; MI25_plants.
DR PANTHER; PTHR37774; PTHR37774; 1.
DR Pfam; PF05405; Mt_ATP-synt_B; 1.
PE 1: Evidence at protein level;
KW ATP synthesis; CF(0); Hydrogen ion transport; Ion transport; Membrane;
KW Mitochondrion; Reference proteome; RNA editing; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..192
FT /note="ATP synthase protein MI25"
FT /id="PRO_0000096473"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 64..65
FT /note="SL -> RI (in Ref. 4; ABS50641/ABS50640)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 192 AA; 21693 MW; C32B18B4C7844F09 CRC64;
MRLSITNMDG RKMLFAAILS ICALSSKKIL IYNEEMIVAL CFIGFIIFSR KSLGTTFKVT
LDGSLQAIQE ELQQFPNPNE VVLLESNEQQ RLLRISLRIC GTVVESLPMA RCAPKCEKTV
QALLCRNLNV KLATLTNAIS SRRIRFQDDL VTKFYTLVGK QFAYSCISKA ERVEFIRESL
VVLRMVRGGV FS
