ID   MI25_TOBAC              Reviewed;         198 AA.
AC   P09003;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=ATP synthase protein MI25;
DE   AltName: Full=ORF 25;
OS   Nicotiana tabacum (Common tobacco).
OG   Mitochondrion.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Coker 139;
RX   PubMed=3447748; DOI=10.1007/bf00434824;
RA   Stamper S.E., Dewey R.E., Bland M.M., Levings C.S. III;
RT   "Characterization of the gene urf13-T and an unidentified reading frame,
RT   ORF 25, in maize and tobacco mitochondria.";
RL   Curr. Genet. 12:457-463(1987).
CC   -!- FUNCTION: This is one of the chains of the nonenzymatic component
CC       (CF(0) subunit) of the mitochondrial ATPase complex. {ECO:0000250}.
CC   -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC       - and CF(0) - the membrane proton channel. CF(1) has five subunits:
CC       alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main
CC       subunits: a, b and c (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}.
CC   -!- RNA EDITING: Modified_positions=50, 165, 187;
CC   -!- SIMILARITY: Belongs to the ATPase protein MI25 family. {ECO:0000305}.
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DR   EMBL; X06310; CAA29629.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S01445; S01445.
DR   RefSeq; YP_173388.1; NC_006581.1.
DR   AlphaFoldDB; P09003; -.
DR   SMR; P09003; -.
DR   GeneID; 3205236; -.
DR   KEGG; nta:3205236; -.
DR   OrthoDB; 1299259at2759; -.
DR   Proteomes; UP000084051; Mitochondrion MT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000276; C:mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); IEA:InterPro.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015986; P:proton motive force-driven ATP synthesis; IEA:InterPro.
DR   InterPro; IPR008688; ATP_synth_Bsub_B/MI25.
DR   InterPro; IPR044988; MI25_plants.
DR   PANTHER; PTHR37774; PTHR37774; 1.
DR   Pfam; PF05405; Mt_ATP-synt_B; 1.
PE   2: Evidence at transcript level;
KW   ATP synthesis; CF(0); Hydrogen ion transport; Ion transport; Membrane;
KW   Mitochondrion; Reference proteome; RNA editing; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..198
FT                   /note="ATP synthase protein MI25"
FT                   /id="PRO_0000096476"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   198 AA;  22436 MW;  A1FD92CD6AECDB43 CRC64;
     MRLSSTNMQA RKMLFAAILS ICASSSKKIS IYNEEMIVAL CFIGFIIFSW KSLGKTFKVT
     LDGRIQAIQE ESQQFPNPNE VVPPESNEQQ RLLRISLRIC GTVVESLPMA RCAPKCEKTV
     QALLCRNLNV KSATLPNATS SRRIRLQDDI AIKMHVLVGK RFCPWCSSKA ERVEFIRESL
     VVLRMVWVGD SLKNKELE