MI4GD_MOUSE
ID MI4GD_MOUSE Reviewed; 222 AA.
AC Q3UBZ5; Q05CE9; Q7TMH3; Q8R333;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=MIF4G domain-containing protein;
GN Name=Mif4gd;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Czech II, FVB/N, and NMRI; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions in replication-dependent translation of histone
CC mRNAs which differ from other eukaryotic mRNAs in that they do not end
CC with a poly-A tail but a stem-loop. May participate in circularizing
CC those mRNAs specifically enhancing their translation (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with EIF4G1, EIF4G2 and SLBP; probably tethered by
CC SLBP to the 3'-end of mRNAs ending with the histone stem-loop, it also
CC interacts with EIF4G1 which is bound to their 5'-end. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UBZ5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UBZ5-2; Sequence=VSP_033878, VSP_033879;
CC -!- SIMILARITY: Belongs to the MIF4GD family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK150749; BAE29819.1; -; mRNA.
DR EMBL; AL645470; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC026740; AAH26740.1; -; mRNA.
DR EMBL; BC026745; AAH26745.1; -; mRNA.
DR EMBL; BC055812; AAH55812.1; -; mRNA.
DR CCDS; CCDS25643.1; -. [Q3UBZ5-1]
DR RefSeq; NP_001230513.1; NM_001243584.1. [Q3UBZ5-1]
DR RefSeq; NP_081438.4; NM_027162.4. [Q3UBZ5-1]
DR AlphaFoldDB; Q3UBZ5; -.
DR SMR; Q3UBZ5; -.
DR BioGRID; 213607; 3.
DR ComplexPortal; CPX-1312; SLBP-SLIP1 complex.
DR IntAct; Q3UBZ5; 1.
DR STRING; 10090.ENSMUSP00000102116; -.
DR iPTMnet; Q3UBZ5; -.
DR PhosphoSitePlus; Q3UBZ5; -.
DR EPD; Q3UBZ5; -.
DR MaxQB; Q3UBZ5; -.
DR PaxDb; Q3UBZ5; -.
DR PRIDE; Q3UBZ5; -.
DR ProteomicsDB; 292317; -. [Q3UBZ5-1]
DR ProteomicsDB; 292318; -. [Q3UBZ5-2]
DR Antibodypedia; 32154; 148 antibodies from 18 providers.
DR DNASU; 69674; -.
DR Ensembl; ENSMUST00000021087; ENSMUSP00000021087; ENSMUSG00000020743. [Q3UBZ5-1]
DR Ensembl; ENSMUST00000106507; ENSMUSP00000102116; ENSMUSG00000020743. [Q3UBZ5-1]
DR GeneID; 69674; -.
DR KEGG; mmu:69674; -.
DR UCSC; uc007mib.1; mouse. [Q3UBZ5-1]
DR UCSC; uc007mie.3; mouse. [Q3UBZ5-2]
DR CTD; 57409; -.
DR MGI; MGI:1916924; Mif4gd.
DR VEuPathDB; HostDB:ENSMUSG00000020743; -.
DR eggNOG; KOG3942; Eukaryota.
DR GeneTree; ENSGT00940000153432; -.
DR HOGENOM; CLU_081010_1_0_1; -.
DR InParanoid; Q3UBZ5; -.
DR OMA; KVNSQRM; -.
DR OrthoDB; 1168859at2759; -.
DR PhylomeDB; Q3UBZ5; -.
DR BioGRID-ORCS; 69674; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Mif4gd; mouse.
DR PRO; PR:Q3UBZ5; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q3UBZ5; protein.
DR Bgee; ENSMUSG00000020743; Expressed in spermatocyte and 224 other tissues.
DR ExpressionAtlas; Q3UBZ5; baseline and differential.
DR Genevisible; Q3UBZ5; MM.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0062073; C:histone mRNA stem-loop binding complex; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0008494; F:translation activator activity; IBA:GO_Central.
DR GO; GO:0002191; P:cap-dependent translational initiation; ISO:MGI.
DR GO; GO:0006446; P:regulation of translational initiation; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR Pfam; PF02854; MIF4G; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome;
KW Translation regulation.
FT CHAIN 1..222
FT /note="MIF4G domain-containing protein"
FT /id="PRO_0000337090"
FT DOMAIN 3..205
FT /note="MIF4G"
FT VAR_SEQ 86..92
FT /note="EYDAREQ -> DWPSLRV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033878"
FT VAR_SEQ 93..222
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033879"
FT CONFLICT 17
FT /note="E -> Q (in Ref. 3; AAH26740)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="G -> S (in Ref. 3; AAH26745/AAH55812)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 222 AA; 25493 MW; 2A0D56790F33701E CRC64;
MSEASRDDYK IQSFDAETQQ LLKTALKDPG AVDLERVANV IVDHSLQDCV FSKEAGRMCY
AIIQAESKQA GQSVFRRGLL NRLQKEYDAR EQLRACSLQG WVCYVTFICN IFDYLRVNNM
PMMALVNPVY DCLFQLAQPE SLSREEEVDC LVLQLHRVGE QLEKMNGQRM DELFILIRDG
FLLPTDLSSL ARLLLLEMIE FRAAGWKTTP AAHKYYYSEV SD
