ID   MIA1_SARMU              Reviewed;         241 AA.
AC   Q26539;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Microneme antigen;
DE   AltName: Full=Lectin SML3;
DE   Flags: Precursor;
OS   Sarcocystis muris.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Sarcocystis.
OX   NCBI_TaxID=5813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 104-126.
RX   PubMed=8801555; DOI=10.1007/s004360050101;
RA   Klein H., Mehlhorn H., Rueger W.;
RT   "Characterization of genomic clones encoding two microneme antigens of
RT   Sarcocystis muris (Apicomplexa).";
RL   Parasitol. Res. 82:230-237(1996).
CC   -!- FUNCTION: Galactose-binding lectin. Plays a role in adhesion to the
CC       host cell. Has a potential role in invasion of host cells.
CC   -!- SUBUNIT: Homodimer or heterodimer of major microneme antigen and
CC       microneme antigen.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle,
CC       microneme.
CC   -!- DEVELOPMENTAL STAGE: Cyst merozoites.
CC   -!- PTM: Contains six disulfide bonds. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the microneme antigen family. {ECO:0000305}.
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DR   EMBL; U21964; AAB18146.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q26539; -.
DR   SMR; Q26539; -.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0020009; C:microneme; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   CDD; cd01100; APPLE_Factor_XI_like; 1.
DR   InterPro; IPR000177; Apple.
DR   InterPro; IPR003609; Pan_app.
DR   Pfam; PF00024; PAN_1; 1.
DR   Pfam; PF14295; PAN_4; 1.
DR   SMART; SM00223; APPLE; 1.
DR   SMART; SM00473; PAN_AP; 1.
DR   PROSITE; PS50948; PAN; 2.
PE   1: Evidence at protein level;
KW   Cell adhesion; Cytoplasmic vesicle; Direct protein sequencing;
KW   Disulfide bond; Lectin; Repeat; Signal.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   PROPEP          35..103
FT                   /evidence="ECO:0000269|PubMed:8801555"
FT                   /id="PRO_0000021722"
FT   CHAIN           104..241
FT                   /note="Microneme antigen"
FT                   /id="PRO_0000021723"
FT   DOMAIN          112..181
FT                   /note="PAN 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DOMAIN          185..241
FT                   /note="PAN 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   REGION          61..92
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..78
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         121
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   BINDING         162
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   BINDING         169
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   BINDING         174
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   DISULFID        112..181
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DISULFID        137..159
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DISULFID        141..147
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DISULFID        185..189
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DISULFID        210..230
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
FT   DISULFID        214..220
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00315"
SQ   SEQUENCE   241 AA;  26510 MW;  E25B1B18A6CA0162 CRC64;
     MRLPIRFPKY VLYGMASAVW SILFLHILVG DTMSAADALS WSGGLIHSPA HRVNVMRSHH
     HEMGKELEQQ HGAEEQQMQR DTKPAAFSNP PHLATGRGPS FVHADGQLDV SCFPHDKNIG
     SRTTEVAVVQ VSSVQDCMKQ CQSRPTCSHF TYNKNSKACH LKDGAPVFYT YNGDMTGPRS
     CDYSCTDNCW MNSETAVKAL DYSGHGPGLC WAACKGTAGC IMYTFKGSTC TLYAKDSFNK
     S