MIAA_HELPS
ID MIAA_HELPS Reviewed; 276 AA.
AC B2UVF6;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=tRNA dimethylallyltransferase;
DE EC=2.5.1.75;
DE AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE Short=DMAPP:tRNA dimethylallyltransferase;
DE Short=DMATase;
DE AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE Short=IPP transferase;
DE Short=IPPT;
DE Short=IPTase;
GN Name=miaA; OrderedLocusNames=HPSH_07210;
OS Helicobacter pylori (strain Shi470).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=512562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Shi470;
RA Kersulyte D., Kalia A., Gilman R.H., Berg D.E.;
RT "Genome sequence of Helicobacter pylori from the remote Amazon: traces of
RT Asian ancestry of the first Americans.";
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC adenine at position 37 in tRNAs that read codons beginning with
CC uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC (i(6)A). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine(37) in tRNA + dimethylallyl diphosphate =
CC diphosphate + N(6)-dimethylallyladenosine(37) in tRNA;
CC Xref=Rhea:RHEA:26482, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10375,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:74411,
CC ChEBI:CHEBI:74415; EC=2.5.1.75;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IPP transferase family. {ECO:0000305}.
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DR EMBL; CP001072; ACD48838.1; -; Genomic_DNA.
DR AlphaFoldDB; B2UVF6; -.
DR SMR; B2UVF6; -.
DR KEGG; hps:HPSH_07210; -.
DR HOGENOM; CLU_032616_0_1_7; -.
DR OMA; YAKRQYT; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR039657; Dimethylallyltransferase.
DR InterPro; IPR018022; IPT.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11088; PTHR11088; 1.
DR TIGRFAMs; TIGR00174; miaA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Magnesium; Nucleotide-binding; Transferase; tRNA processing.
FT CHAIN 1..276
FT /note="tRNA dimethylallyltransferase"
FT /id="PRO_0000377188"
FT REGION 9..12
FT /note="Interaction with substrate tRNA"
FT /evidence="ECO:0000250"
FT SITE 73
FT /note="Interaction with substrate tRNA"
FT /evidence="ECO:0000250"
SQ SEQUENCE 276 AA; 31088 MW; A31F323F8FBF5BBB CRC64;
MDAEIFSLDS LSIYKDINIA SAKPSLKERK NIKHYALDYL SIDEKNNAPL FKTLLEDAMR
VSSKEVLLIV GGSSFYLKSI LEGLSDTPKI SGEEAVKIEQ EINSLANPYA FLKSIDPTSA
FKIHPNDTYR IHKALEIFYS THTPPSEYFK TNPKKPFEHA ISLFALSIEK SALANNIKQR
TKNMLDCGLI EEIKALYAQY PKDSQPFKAI GVKESILFLE KQLTLKKLEE AIISNTIQLA
KRQNTFNKTQ FNNLYTGSVG EVRHAILNHS KSAIKG