ARLY_METAC
ID ARLY_METAC Reviewed; 491 AA.
AC Q8TR65;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Argininosuccinate lyase {ECO:0000255|HAMAP-Rule:MF_00006};
DE Short=ASAL {ECO:0000255|HAMAP-Rule:MF_00006};
DE EC=4.3.2.1 {ECO:0000255|HAMAP-Rule:MF_00006};
DE AltName: Full=Arginosuccinase {ECO:0000255|HAMAP-Rule:MF_00006};
GN Name=argH {ECO:0000255|HAMAP-Rule:MF_00006}; OrderedLocusNames=MA_1318;
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(N(omega)-L-arginino)succinate = fumarate + L-arginine;
CC Xref=Rhea:RHEA:24020, ChEBI:CHEBI:29806, ChEBI:CHEBI:32682,
CC ChEBI:CHEBI:57472; EC=4.3.2.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00006};
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC from L-ornithine and carbamoyl phosphate: step 3/3. {ECO:0000255|HAMAP-
CC Rule:MF_00006}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00006}.
CC -!- SIMILARITY: Belongs to the lyase 1 family. Argininosuccinate lyase
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00006}.
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DR EMBL; AE010299; AAM04735.1; -; Genomic_DNA.
DR RefSeq; WP_011021337.1; NC_003552.1.
DR AlphaFoldDB; Q8TR65; -.
DR SMR; Q8TR65; -.
DR STRING; 188937.MA_1318; -.
DR EnsemblBacteria; AAM04735; AAM04735; MA_1318.
DR GeneID; 1473206; -.
DR KEGG; mac:MA_1318; -.
DR HOGENOM; CLU_027272_2_3_2; -.
DR InParanoid; Q8TR65; -.
DR OMA; KKNPDVF; -.
DR OrthoDB; 51806at2157; -.
DR PhylomeDB; Q8TR65; -.
DR UniPathway; UPA00068; UER00114.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004056; F:argininosuccinate lyase activity; IBA:GO_Central.
DR GO; GO:0042450; P:arginine biosynthetic process via ornithine; IBA:GO_Central.
DR CDD; cd01359; Argininosuccinate_lyase; 1.
DR Gene3D; 1.10.275.10; -; 1.
DR HAMAP; MF_00006; Arg_succ_lyase; 1.
DR InterPro; IPR029419; Arg_succ_lyase_C.
DR InterPro; IPR009049; Argininosuccinate_lyase.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR000362; Fumarate_lyase_fam.
DR InterPro; IPR022761; Fumarate_lyase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR PANTHER; PTHR43814; PTHR43814; 1.
DR Pfam; PF14698; ASL_C2; 1.
DR Pfam; PF00206; Lyase_1; 1.
DR PRINTS; PR00149; FUMRATELYASE.
DR SUPFAM; SSF48557; SSF48557; 1.
DR TIGRFAMs; TIGR00838; argH; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; Cytoplasm; Lyase;
KW Reference proteome.
FT CHAIN 1..491
FT /note="Argininosuccinate lyase"
FT /id="PRO_0000137861"
SQ SEQUENCE 491 AA; 54705 MW; C30058E8B2528ED3 CRC64;
MSNILRRGRL EAAPDEEILR YASSMETDRW IFSADIAVDL AHTVMLKEQG IISAEDCSKI
LAGLLKIREE GMEKLDFSYE DIHISLESRL IDMVGEDVGG RMHSGRSRND EVATCIRLTL
REELLGLLEE IFALRKTLVS LAEKHTETLM PGFTHLQHAQ PTTLAHHLCA HEAALGRDFD
RVQDAFSRVN LCPLGAAAFA STGFNLNRKR TQELLGFEGL LENSMDAVSS RDFLIECASV
FSNLMINLSR MAEELVIWSS SEFNFIELDD TYASTSSIMP QKKNPDTAEL MRGKTGVAVG
ALMSLLTICK GLPLSYNRDL QEATPNIWRS VETVRASVRV MEGMVRTMKI HPEVLSAQSV
TGFTTATELA DTFVREAGIP FRTAHQIVGM LAREGEKPTI EKIDSVAEIV LGESLSSRGL
TEKMIKEALN PVSNIKRRKI EGGPAPEEMQ HYIGRQQTEL ELNEQEIATI KDSIDSAFEA
LLEVVDEYRK V