MIBS_STRA7
ID MIBS_STRA7 Reviewed; 440 AA.
AC A3KI17; A0A0K2AKB9;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=2-methylisoborneol synthase;
DE Short=2-MIB synthase;
DE EC=4.2.3.118;
GN ORFNames=SAM23877_0409 {ECO:0000312|EMBL:AKZ53458.1},
GN SAML0357 {ECO:0000312|EMBL:CAJ89344.1};
OS Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 /
OS NBRC 12836 / NRRL B-2516).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=278992;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX PubMed=16956972; DOI=10.1093/molbev/msl108;
RA Choulet F., Aigle B., Gallois A., Mangenot S., Gerbaud C., Truong C.,
RA Francou F.-X., Fourrier C., Guerineau M., Decaris B., Barbe V.,
RA Pernodet J.-L., Leblond P.;
RT "Evolution of the terminal regions of the Streptomyces linear chromosome.";
RL Mol. Biol. Evol. 23:2361-2369(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX PubMed=26410452; DOI=10.1016/j.jbiotec.2015.09.020;
RA Thibessard A., Haas D., Gerbaud C., Aigle B., Lautru S., Pernodet J.L.,
RA Leblond P.;
RT "Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
RT spiramycin producer.";
RL J. Biotechnol. 214:117-118(2015).
RN [3]
RP FUNCTION IN 2-METHYLISOBORNEOL BIOSYNTHESIS, AND PATHWAY.
RC STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX PubMed=18492804; DOI=10.1073/pnas.0802312105;
RA Komatsu M., Tsuda M., Omura S., Oikawa H., Ikeda H.;
RT "Identification and functional analysis of genes controlling biosynthesis
RT of 2-methylisoborneol.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:7422-7427(2008).
CC -!- FUNCTION: Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-
CC MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the
CC intermediacy of 2-methyllinalyl diphosphate.
CC {ECO:0000305|PubMed:18492804}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-2-methylgeranyl diphosphate + H2O = 2-methylisoborneol +
CC diphosphate; Xref=Rhea:RHEA:32571, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:61984, ChEBI:CHEBI:61987;
CC EC=4.2.3.118;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- MISCELLANEOUS: 2-MIB is a volatile organic compound that has an
CC unusually low odor threshold. Together with geosmin, methylisoborneol
CC is responsible for the characteristic smell of moist soil as well as
CC unpleasant taste and odor episodes associated with public water
CC supplies and contamination of various foodstuffs, including fish, wine,
CC and beer.
CC -!- SIMILARITY: Belongs to the terpene synthase family. 2-methylisoborneol
CC synthase subfamily. {ECO:0000305}.
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DR EMBL; AM238663; CAJ89344.1; -; Genomic_DNA.
DR EMBL; CP012382; AKZ53458.1; -; Genomic_DNA.
DR RefSeq; WP_053126184.1; NZ_CP012382.1.
DR AlphaFoldDB; A3KI17; -.
DR SMR; A3KI17; -.
DR STRING; 1889.SAM40697_0346; -.
DR EnsemblBacteria; AKZ53458; AKZ53458; SAM23877_0409.
DR KEGG; ag:CAJ89344; -.
DR KEGG; samb:SAM23877_0409; -.
DR OMA; VDGNHHW; -.
DR OrthoDB; 1869158at2; -.
DR Proteomes; UP000061018; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0010333; F:terpene synthase activity; ISS:UniProtKB.
DR GO; GO:0042214; P:terpene metabolic process; IDA:UniProtKB.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034686; Terpene_cyclase-like_2.
DR SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Lyase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..440
FT /note="2-methylisoborneol synthase"
FT /id="PRO_0000403384"
FT REGION 1..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 197
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 198
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 202
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 353
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
SQ SEQUENCE 440 AA; 47959 MW; 8FF1D4CEDC0818A0 CRC64;
MPDSGPLGPH SPDHRPTPAT TVPDAPASKP PDVAVTPTAS EFLAALHPPV PIPSPSPPSG
SASAAADTPD ATTVGSALQR ILRGPTGPGT AALALSVRHD PPSLPGSPAP AEPAAGRAVP
GLYHHPVPEP DPARVEEVSR RIKRWAEDEV QLYPEDWEGE FDGFSVGRYM VACHPDAPTV
DHLMLATRLM VAENAVDDCY CEDHGGSPVG LGGRLLLAHT AIDPFHTTAE YAPPWRESLT
SDAPRRAYRS AMDYFVRAAT PSQADRYRHD MARLHLGYLA EAAWAQTDHV PEVWEYLAMR
QFNNFRPCPT ITDTVGGYEL PADLHARPDM QRVIALAGNA TTIVNDLYSY TKELDSPGRH
LNLPVVIAER ERLSERDAYL KAVEVHNELQ HAFEAAAAEL AKACPLPTVL RFLKGVAAWV
DGNHDWHRTN TYRYSLPDFW
