ID   MIBS_STRGR              Reviewed;         437 AA.
AC   Q9F1V8;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=2-methylisoborneol synthase;
DE            Short=2-MIB synthase;
DE            EC=4.2.3.118;
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=13189;
RA   Watanabe M., Kawamoto S., Ochi K.;
RT   "S.griseus rbsC, rbsA, glk, secreted protein, p52(p3), uk, erg6
RT   homologues.";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-
CC       MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the
CC       intermediacy of 2-methyllinalyl diphosphate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-2-methylgeranyl diphosphate + H2O = 2-methylisoborneol +
CC         diphosphate; Xref=Rhea:RHEA:32571, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:61984, ChEBI:CHEBI:61987;
CC         EC=4.2.3.118;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- MISCELLANEOUS: 2-MIB is a volatile organic compound that has an
CC       unusually low odor threshold. Together with geosmin, methylisoborneol
CC       is responsible for the characteristic smell of moist soil as well as
CC       unpleasant taste and odor episodes associated with public water
CC       supplies and contamination of various foodstuffs, including fish, wine,
CC       and beer.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. 2-methylisoborneol
CC       synthase subfamily. {ECO:0000305}.
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DR   EMBL; AB040071; BAB20507.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F1V8; -.
DR   SMR; Q9F1V8; -.
DR   BRENDA; 4.2.3.118; 12251.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0010333; F:terpene synthase activity; ISS:UniProtKB.
DR   GO; GO:0042214; P:terpene metabolic process; ISS:UniProtKB.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034686; Terpene_cyclase-like_2.
DR   SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase; Magnesium; Metal-binding.
FT   CHAIN           1..437
FT                   /note="2-methylisoborneol synthase"
FT                   /id="PRO_0000205355"
FT   REGION          32..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..72
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         194
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         195
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         199
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         342
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         346
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         350
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   437 AA;  47558 MW;  DF7BA9ADE1D19508 CRC64;
     MPVPELPPPR SSLPEAVTRF GASVLGAVAA RAHDSEATVG GPSGGRPLPS PPAGLSFGPP
     SPAAPPTDVP APEAPGWGAD LERLLCGPHG LGTAGLRLTP GKERPVPATA REGRPIPGLY
     HHPVPEPDEA RVEEVSRRIK AWALDEVSLY PEEWEEQFDG FSVGRYMVGC HPDAPTVDHL
     MLATRLMVAE NAVDDCYCED HGGSPVGLGE RLLLAHTALD PLYTAREYQP GWAASLHADA
     PRRAYRSAMD YFVRAAGPSQ ADRLRHDMAR LHLGYLAEAA WAQQDQVPEV WEYLAMRQFN
     NFRPCPTITD TVGGYELPAD LHAQAAMQKV IALASNATTI VNDLYSYTKE LAAPGRHLNL
     PVVIAEREGL SDQDAYLKSV EIHNELMHAF ESEAAALAAA CPVPSVQRFL RGVAAWVDGN
     HHWHRSNTYR YSLPDFW