ID   MIBS_STRLS              Reviewed;         481 AA.
AC   D3KYU2;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=2-methylisoborneol synthase;
DE            Short=2-MIB synthase;
DE            EC=4.2.3.118;
GN   Name=tpc;
OS   Streptomyces lasalocidi (Streptomyces lasaliensis).
OG   Plasmid pKSL.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=324833;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31180 / DSM 41442 / NRRL 3382 / X-537;
RA   Oikawa H., Tsuda M., Migita A.;
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND PATHWAY.
RC   STRAIN=ATCC 31180 / DSM 41442 / NRRL 3382 / X-537;
RX   PubMed=18492804; DOI=10.1073/pnas.0802312105;
RA   Komatsu M., Tsuda M., Omura S., Oikawa H., Ikeda H.;
RT   "Identification and functional analysis of genes controlling biosynthesis
RT   of 2-methylisoborneol.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7422-7427(2008).
CC   -!- FUNCTION: Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-
CC       MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the
CC       intermediacy of 2-methyllinalyl diphosphate.
CC       {ECO:0000269|PubMed:18492804}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-2-methylgeranyl diphosphate + H2O = 2-methylisoborneol +
CC         diphosphate; Xref=Rhea:RHEA:32571, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:61984, ChEBI:CHEBI:61987;
CC         EC=4.2.3.118; Evidence={ECO:0000269|PubMed:18492804};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000305|PubMed:18492804};
CC   -!- MISCELLANEOUS: 2-MIB is a volatile organic compound that has an
CC       unusually low odor threshold. Together with geosmin, methylisoborneol
CC       is responsible for the characteristic smell of moist soil as well as
CC       unpleasant taste and odor episodes associated with public water
CC       supplies and contamination of various foodstuffs, including fish, wine,
CC       and beer.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. 2-methylisoborneol
CC       synthase subfamily. {ECO:0000305}.
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DR   EMBL; AB547324; BAI77523.1; -; Genomic_DNA.
DR   AlphaFoldDB; D3KYU2; -.
DR   SMR; D3KYU2; -.
DR   KEGG; ag:BAI77523; -.
DR   BRENDA; 4.2.3.118; 12665.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0010333; F:terpene synthase activity; IDA:UniProtKB.
DR   GO; GO:0042214; P:terpene metabolic process; IDA:UniProtKB.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034686; Terpene_cyclase-like_2.
DR   SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Lyase; Magnesium; Metal-binding; Plasmid.
FT   CHAIN           1..481
FT                   /note="2-methylisoborneol synthase"
FT                   /id="PRO_0000403385"
FT   REGION          1..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          139..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..25
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..104
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         238
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         239
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         243
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         386
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         390
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         394
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   481 AA;  51628 MW;  78723282CBA5A7EA CRC64;
     MPDSGSLGPP TSLPEQPPAP PATAPDAPAA TVTDRPVTSS VAHFLAGLHP PVTRPSSPPS
     PSMPPASSNP SSPPSSSMPP ASWAPPSPLS PPAPSLPPTS PPATAPETSA ATGSDSVVRR
     VPVGPTGLGT TALSLARRQA AVPPDAVPAP SGPSAEGPVV PGLYHHPIPE PDPVRVAEVS
     RRIKRWAEDE VRLYPEEWEG QFDGFSVGRY MVACHPDAPT VDHLMLATRL MVAENAVDDC
     YCEDHGGSPV GLGGRLLLAH TALDHLHTTA EYAPEWSESL GSDAPRRAYR SAMDHFVRAA
     TPSQADRYRH DMARLHLGYL AEAAWAETGH VPEVCEYLAM RQFNNFRPCP TITDTVGGYE
     LPADLHARPD MQRVIALAGN ATTIVNDLYS YTKELDSPGR HLNLPVVIAE REHLSDRDAY
     LKAVEVHNEL MHAFEAAAAE LAADCPVPAV LRFLRGVAAW VDGNHDWHRT NTYRYSLPDF
     W