ID   MIC27_PONAB             Reviewed;         266 AA.
AC   Q5NVS6;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=MICOS complex subunit MIC27;
DE   AltName: Full=Apolipoprotein O-like;
DE   AltName: Full=Protein FAM121A;
DE   Flags: Precursor;
GN   Name=APOOL; Synonyms=FAM121A, MIC27;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Specifically binds to
CC       cardiolipin (in vitro) but not to the precursor lipid
CC       phosphatidylglycerol. Plays a crucial role in crista junction formation
CC       and mitochondrial function. {ECO:0000250|UniProtKB:Q6UXV4}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex, composed of at least MICOS10/MIC10,
CC       CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26
CC       and MICOS13/MIC13. This complex was also known under the names MINOS or
CC       MitOS complex. The MICOS complex associates with mitochondrial outer
CC       membrane proteins SAMM50, MTX1 and MTX2 (together described as
CC       components of the mitochondrial outer membrane sorting assembly
CC       machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane
CC       protein TMEM11 and with HSPA9. The MICOS and SAM complexes together
CC       with DNAJC11 are part of a large protein complex spanning both
CC       membranes termed the mitochondrial intermembrane space bridging (MIB)
CC       complex. Interacts with MICOS10/MIC10, IMMT/MIC60 and APOO/MIC23/MIC26.
CC       {ECO:0000250|UniProtKB:Q6UXV4}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q6UXV4}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q6UXV4}. Mitochondrion
CC       {ECO:0000250|UniProtKB:Q6UXV4}.
CC   -!- SIMILARITY: Belongs to the apolipoprotein O/MICOS complex subunit Mic27
CC       family. {ECO:0000305}.
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DR   EMBL; CR925925; CAI29587.1; -; mRNA.
DR   RefSeq; NP_001127062.1; NM_001133590.1.
DR   AlphaFoldDB; Q5NVS6; -.
DR   GeneID; 100174091; -.
DR   KEGG; pon:100174091; -.
DR   CTD; 139322; -.
DR   InParanoid; Q5NVS6; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0061617; C:MICOS complex; IEA:InterPro.
DR   GO; GO:0042407; P:cristae formation; IEA:InterPro.
DR   InterPro; IPR019166; MIC26/MIC27.
DR   InterPro; IPR033182; MIC26/MIC27_animal.
DR   PANTHER; PTHR14564; PTHR14564; 1.
DR   Pfam; PF09769; ApoO; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..266
FT                   /note="MICOS complex subunit MIC27"
FT                   /id="PRO_0000042053"
FT   TOPO_DOM        28..110
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        130..137
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        156..266
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         204
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6UXV4"
SQ   SEQUENCE   266 AA;  28710 MW;  DC7345789053F8A1 CRC64;
     MAAIRMGKLT TMPAGLIYAS VSVHAAKEEE SKKQLVKPEQ LPIYTAPPLQ SKYVEEQPGH
     LQMGFASIRT ATGCYIGWCK GVYVFVKNGI MDTVQFGKDA YVYMKNPPRD FLPKMGVITV
     SGLAGLVSAR KGSKFKKITY PLGLATLGAT VCYPVQSVII AKVTAKKIYA TSQQIFGAVK
     SLWTKSSKEE SLPKPKEKTK LGSSSEIEVP AKTTHVLKHS VPLPTELSSE AKTKSESTSD
     VQISEVGGKI IGTWGPNVTN SGVLRI