ID   MIC60_AJECG             Reviewed;         685 AA.
AC   C0NUJ9;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; ORFNames=HCBG_07030;
OS   Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
OS   (Darling's disease fungus) (Histoplasma capsulatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma.
OX   NCBI_TaxID=447093;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G186AR / H82 / ATCC MYA-2454 / RMSCC 2432;
RA   Champion M., Cuomo C.A., Ma L.-J., Henn M.R., Sil A., Goldman B.,
RA   Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A.,
RA   Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J.,
RA   Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA   Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA   Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C.,
RA   Klein B., McEwen J.G., Puccia R., Goldman G.H., Felipe M.S., Nino-Vega G.,
RA   San-Blas G., Taylor J., Mendoza L., Galagan J.E., Nusbaum C., Birren B.W.;
RT   "The genome sequence of Ajellomyces capsulatus strain G186AR.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; GG663372; EEH05079.1; -; Genomic_DNA.
DR   AlphaFoldDB; C0NUJ9; -.
DR   SMR; C0NUJ9; -.
DR   STRING; 447093.C0NUJ9; -.
DR   EnsemblFungi; EEH05079; EEH05079; HCBG_07030.
DR   VEuPathDB; FungiDB:HCBG_07030; -.
DR   HOGENOM; CLU_008024_1_2_1; -.
DR   InParanoid; C0NUJ9; -.
DR   OrthoDB; 1540241at2759; -.
DR   Proteomes; UP000001631; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 1.
DR   Pfam; PF09731; Mitofilin; 2.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..42
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           43..685
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406637"
FT   TOPO_DOM        43..145
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        166..685
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          66..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          283..307
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          356..494
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        66..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   685 AA;  75882 MW;  BFF0AE625EB9ED9C CRC64;
     MLRTSIASSR PLLPSSVCPK APAQWLCTSR ARRVNTATRR YHALARKSNA GRRCSLTPNT
     ATTSQFFQKA ASSTSTKPPG PSDADVRSPA SPSSRSSLRP ESIPKPPQSP PVQGQTSPGS
     EVLPPDHESS TPPPPPGPKS SRLRKLLYLF LTAGLAYAGG VWYSLRSDNF YDFFTEYIPY
     GEEAVLYLEE RDFRNRFPHV TKQINRRVTV PKDEGAQVTI PSGSGLSWKV AEEQQEATDM
     TKKGRRMGTA HANEPTKDIK VAEKAKEEVK SKSAAKKEDV AANIPIQEAL EPQPAKTEEK
     NLEAPRQPAV PAVTAIERLV QDKADEPVVQ DLVKVFNDVI SVISADESAS KFAGPIAKAK
     EELQRIGDRI VALKKDAQES AQEEIRNAHA AFDKSAAELI RRIDEVRTQD AAEFREEFES
     EREKIARSYQ EKVNTELQRA HEVAEQRLRN ELVEQAIELN RKFLSDVKTL VENEREGRLS
     KLAELSANVA ELERLTAGWS DVVDINLKTQ QLQVAVDAVR TTLENSDVPR PFVRELAAVK
     ELASNDEVVA AAIASISPAA YQRGIPSAAQ LVDRFRRVAS EVRKARLLPE NAGITSHAAS
     LVLSKVMLKK QGLPTSDDVE SILTRTENFL EEGNFDEAAR EMNSLQGWAK LLSKDWLADV
     RRVLEVKQAL EIIETEARLR CLQVE