ID   MIC60_AJECH             Reviewed;         686 AA.
AC   C6H203;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; ORFNames=HCDG_00735;
OS   Ajellomyces capsulatus (strain H143) (Darling's disease fungus)
OS   (Histoplasma capsulatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma.
OX   NCBI_TaxID=544712;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H143;
RA   Champion M., Cuomo C.A., Ma L.-J., Henn M.R., Sil A., Goldman B.,
RA   Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A.M.,
RA   Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J.,
RA   Griggs A., Gujja S., Heiman D.I., Hepburn T.A., Howarth C., Jen D.,
RA   Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S.,
RA   Shea T.D., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J.,
RA   Yandava C., Klein B., McEwen J.G., Puccia R., Goldman G.H., Felipe M.S.,
RA   Nino-Vega G., San-Blas G., Taylor J.W., Mendoza L., Galagan J.E.,
RA   Nusbaum C., Birren B.W.;
RT   "The genome sequence of Ajellomyces capsulatus strain H143.";
RL   Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; GG692419; EER45156.1; -; Genomic_DNA.
DR   AlphaFoldDB; C6H203; -.
DR   SMR; C6H203; -.
DR   STRING; 544712.C6H203; -.
DR   EnsemblFungi; EER45156; EER45156; HCDG_00735.
DR   VEuPathDB; FungiDB:HCDG_00735; -.
DR   eggNOG; KOG1854; Eukaryota.
DR   HOGENOM; CLU_008024_1_2_1; -.
DR   OMA; LQGWAKV; -.
DR   Proteomes; UP000002624; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 1.
DR   Pfam; PF09731; Mitofilin; 2.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..42
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           43..686
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406638"
FT   TOPO_DOM        43..146
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        167..686
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          66..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          287..307
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          357..495
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        66..124
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   686 AA;  75972 MW;  9CEA4C27CE77ADDF CRC64;
     MLRTSIASSR PLLPSSVCPK APAQWLCTSR ARRVNTATRR YHALARKSNA GRRCPLTPNT
     ATTSQFFQKA ASSTSTKPPG PSDADVRSPA SPSSRFSLRP ESIPKTPQSP PVQGQTSPGS
     EVLPPDHESS TPPPPLEGPK SSRLRKLLYL FLTAGLAYAG GVWYSLRSDN FYDFFTEYIP
     YGEEAVLYLE ERDFRNRFPH VTKQINRRVT VPKDEGAQVT IPSGSGLSWK VAEEQQEATD
     MTKKGRRMGT AHANEPTKDI KVAEKAKEEV KSKSAAKKED VAANIPIQEA LEPQPAKAEE
     KNLEAPRQPA VPAVTAIERL VQDKVDEPVV QDLVKVFNDV ISVISADESA SKFAGPIAKA
     KEELQRIGDR IVALKKDAQE SAQEEIRNAH AAFDKSAAEL IRRIDEVRTQ DAAEFREEFE
     SEREKIARSY QEKVNTELQR AHEVAEQRLR NELVEQAIEL NRKFLSDVKT LVENEREGRL
     SKLAELSANV AELERLTAGW SDVVDINLKT QQLQVAVDAV RTTLENSDVP RPFVRELAAV
     KELASNDEVV AAAIASISPA AYQRGIPSAA QLVDRFRRVA SEVRKASLLP ENAGITSHAA
     SLVLSKVMLK KQGLPTSDDV ESILTRTANF LEEGNFDEAA REMNSLQGWA KLLSKDWLAD
     VRRVLEVKQA LEIIETEARL RCLQVE