ID   MIC60_ASHGO             Reviewed;         496 AA.
AC   Q754G4;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; OrderedLocusNames=AFR106C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016819; AAS53477.2; -; Genomic_DNA.
DR   RefSeq; NP_985653.2; NM_211007.2.
DR   AlphaFoldDB; Q754G4; -.
DR   SMR; Q754G4; -.
DR   STRING; 33169.AAS53477; -.
DR   EnsemblFungi; AAS53477; AAS53477; AGOS_AFR106C.
DR   GeneID; 4621896; -.
DR   KEGG; ago:AGOS_AFR106C; -.
DR   eggNOG; KOG1854; Eukaryota.
DR   HOGENOM; CLU_008024_2_0_1; -.
DR   InParanoid; Q754G4; -.
DR   OMA; NRWNLLE; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0061617; C:MICOS complex; IBA:GO_Central.
DR   GO; GO:0030061; C:mitochondrial crista; IEA:EnsemblFungi.
DR   GO; GO:0044284; C:mitochondrial crista junction; IEA:EnsemblFungi.
DR   GO; GO:0042407; P:cristae formation; IBA:GO_Central.
DR   GO; GO:0045041; P:protein import into mitochondrial intermembrane space; IEA:EnsemblFungi.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 1.
DR   Pfam; PF09731; Mitofilin; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..14
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           15..496
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406643"
FT   TOPO_DOM        15..35
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..496
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   COILED          216..261
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   496 AA;  55227 MW;  0DA6D75C80FFD97E CRC64;
     MLSSRAAAFT GRRLASTLPP VPRKKSHGVR RLLAKAVVAT SLFYAGGLTL SAYNDKANEL
     FVEHVPFGEE LVERWEDWTS LRRPGRRMID ARRVDEISRD FRAAATPEAT PVVVRPLVQL
     QLPELQMQGS SPVLEALVNN VNDVVVALNA RALELPEDTA SALSSVYGEI VHSIQALNAS
     LDQEFATEVE SRTGKAISSV QEQLEVEYKQ RELALAEQYI QNFEVFKSQL QKATAEQLET
     ELKAHEQALL ARHRNEVAQL SIRQVEEFNK IIEKKLDQER NGRLAKLSEL NSAVESLAPV
     LDRLELRAVK NECVTQLSTL ISDIQGKLSR GGDEPLDLSS DLQRLTLLAD ILPRPKRCCS
     EGPALLDVAM AELQAKAQAP VASNEQLYNR WQLLQPELKT TSLLPPNAGF LGHLTAKLFS
     MLLFTKEGFS TTQDMDAVTA RIAENLRLNK LDCALEEAVN MKGWSRKSAD AWVDLARRRL
     EVLTLLDVIE AEVKTL