MIC60_DROME
ID MIC60_DROME Reviewed; 739 AA.
AC P91928; Q8IN13; Q9V3K0;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 4.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=MICOS complex subunit Mic60;
GN Name=Mitofilin {ECO:0000312|FlyBase:FBgn0019960};
GN ORFNames=CG6455 {ECO:0000312|FlyBase:FBgn0019960};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAN13897.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-409 (ISOFORM C).
RC TISSUE=Ovary;
RX PubMed=10071211; DOI=10.1007/s004380050942;
RA Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R.,
RA Barsanti P.;
RT "Identification of nuclear genes encoding mitochondrial proteins: isolation
RT of a collection of D. melanogaster cDNAs homologous to sequences in the
RT Human Gene Index database.";
RL Mol. Gen. Genet. 261:64-70(1999).
RN [5]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=24998521; DOI=10.1016/j.biocel.2014.05.041;
RA Banerjee S., Chinthapalli B.;
RT "A proteomic screen with Drosophila Opa1-like identifies Hsc70-5/Mortalin
RT as a regulator of mitochondrial morphology and cellular homeostasis.";
RL Int. J. Biochem. Cell Biol. 54:36-48(2014).
CC -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC the mitochondrial inner membrane that plays crucial roles in the
CC maintenance of crista junctions, inner membrane architecture, and
CC formation of contact sites to the outer membrane (By similarity).
CC {ECO:0000250|UniProtKB:Q16891}.
CC -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC organizing system (MICOS) complex (By similarity). Interacts with the
CC mitochondria-shaping protein Opa1 (PubMed:24998521).
CC {ECO:0000250|UniProtKB:Q16891, ECO:0000269|PubMed:24998521}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:24998521}; Single-pass membrane protein
CC {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C;
CC IsoId=P91928-1; Sequence=Displayed;
CC Name=B {ECO:0000303|PubMed:10731132};
CC IsoId=P91928-2; Sequence=VSP_007006;
CC -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA71851.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF55943.3; -; Genomic_DNA.
DR EMBL; AE014297; AAN13897.1; -; Genomic_DNA.
DR EMBL; AF145623; AAD38598.1; -; mRNA.
DR EMBL; Y10910; CAA71851.1; ALT_INIT; mRNA.
DR RefSeq; NP_001262818.1; NM_001275889.1. [P91928-1]
DR RefSeq; NP_524444.3; NM_079720.3. [P91928-1]
DR AlphaFoldDB; P91928; -.
DR SMR; P91928; -.
DR BioGRID; 67548; 16.
DR DIP; DIP-23966N; -.
DR IntAct; P91928; 3.
DR MINT; P91928; -.
DR STRING; 7227.FBpp0288710; -.
DR PaxDb; P91928; -.
DR PRIDE; P91928; -.
DR EnsemblMetazoa; FBtr0290271; FBpp0288710; FBgn0019960. [P91928-1]
DR EnsemblMetazoa; FBtr0335199; FBpp0307186; FBgn0019960. [P91928-1]
DR GeneID; 42587; -.
DR KEGG; dme:Dmel_CG6455; -.
DR CTD; 42587; -.
DR FlyBase; FBgn0019960; Mitofilin.
DR VEuPathDB; VectorBase:FBgn0019960; -.
DR eggNOG; KOG1854; Eukaryota.
DR GeneTree; ENSGT00390000002313; -.
DR InParanoid; P91928; -.
DR OMA; STYEAFS; -.
DR PhylomeDB; P91928; -.
DR BioGRID-ORCS; 42587; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42587; -.
DR PRO; PR:P91928; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0019960; Expressed in adult hindgut (Drosophila) and 36 other tissues.
DR ExpressionAtlas; P91928; baseline and differential.
DR Genevisible; P91928; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0061617; C:MICOS complex; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0043008; F:ATP-dependent protein binding; IPI:FlyBase.
DR GO; GO:0019901; F:protein kinase binding; IPI:FlyBase.
DR GO; GO:0042407; P:cristae formation; IBA:GO_Central.
DR GO; GO:0070584; P:mitochondrion morphogenesis; IMP:FlyBase.
DR GO; GO:0007005; P:mitochondrion organization; IMP:FlyBase.
DR InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR PANTHER; PTHR15415; PTHR15415; 1.
DR Pfam; PF09731; Mitofilin; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..739
FT /note="MICOS complex subunit Mic60"
FT /id="PRO_0000084183"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 23..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 283..339
FT /evidence="ECO:0000255"
FT COMPBIAS 23..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 164..191
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..518
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_007006"
FT CONFLICT 25
FT /note="N -> Y (in Ref. 4; CAA71851)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="E -> D (in Ref. 4; CAA71851)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="E -> K (in Ref. 4; CAA71851)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 739 AA; 82014 MW; B278194F3F2D7879 CRC64;
MYRLAVRDQC KCALQRTLQQ TTANNRQFGG SSSGSGGREQ GRRQQEEQGQ QGDQGYQGYQ
SLPPHMREAG FGKVVLFVSP LAAVGGVITY AKYDDDFRKL VEKNVPGAGS VIKVALQEEP
PFKGITKNVN DQIDKVKSGI ETVTSTVDSV TSKVTGLFGG GSGDDKSKKS KVEPVKATPA
EEKRPSKPSE VSKTEAKPVS KPAAAAAPAP AAKPKDNPLP RDVVELEKAI ELSAQLAVKE
YNVAIGVLKG FNDDVRKVVD KAVENGENSL WTTLKNRASA RDTAVATAER AAREAQEKIV
ACEIALSAAA TAQNAKKVEA VRDKIKKLVD HIGNVKDELY RHKDTASVSD KYWRNVEKAR
NYFIDEIESI FPGLSLADKK LNLSKEDLDL FILHAYTHVL AYQKELQRLQ TDGELRLKRA
IDSVRGDNDS EALRAQLEYH LEAERRKLAV ENQKKIFHIH AESDKLLRLQ LKKQAEAHAD
HIKDIVAQRE TDLTRSFKRE LEDKLATEKA NYKLQLAGML GKLRGMDAAL AERADAERTA
NQAQALWAAC QALWASVRAA TPGVHYKDRL RPLKNEINAI AKVAKGDDLV AAVLESVPKE
AQERGVYPED ALRERFLNVE RVARRLALVP EEGAGLPIYF LSYLQSLFIL RPDNPISKDE
LENKPFDYSK LDTYDILNRA RYHVDRSDFL QALKYMNLLQ GASRKIAGEW MKEARLMLET
QQAANTLMAH AAASGLLYL
