ID   MIC60_KOMPG             Reviewed;         509 AA.
AC   C4QXN2;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; OrderedLocusNames=PAS_chr1-4_0172;
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864;
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; FN392319; CAY68005.1; -; Genomic_DNA.
DR   RefSeq; XP_002490286.1; XM_002490241.1.
DR   AlphaFoldDB; C4QXN2; -.
DR   SMR; C4QXN2; -.
DR   STRING; 644223.C4QXN2; -.
DR   EnsemblFungi; CAY68005; CAY68005; PAS_chr1-4_0172.
DR   GeneID; 8197156; -.
DR   KEGG; ppa:PAS_chr1-4_0172; -.
DR   eggNOG; KOG1854; Eukaryota.
DR   HOGENOM; CLU_008024_2_0_1; -.
DR   InParanoid; C4QXN2; -.
DR   OMA; LQGWAKV; -.
DR   Proteomes; UP000000314; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 1.
DR   Pfam; PF09731; Mitofilin; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..19
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..509
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406670"
FT   TOPO_DOM        20..34
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        55..509
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   COILED          202..257
FT                   /evidence="ECO:0000255"
FT   COILED          293..330
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   509 AA;  57129 MW;  53184EA77805BA73 CRC64;
     MLRTIGTKAS GITFRGVRFQ STNPTVKKSK PLRKFLFRLG LLTGVFYAGG VAVSLKNDIV
     QDAFIEHVPL GEALLDFTEY YVNHPEELSF SSTKQKLQNF DKTVLIPKRG VQSAKVEDVE
     HIKNVTRGSA DESVSLSKTI YSNLNLPSID LEFKDEVLQS SVEHLNHLID TIRTQVNTVD
     LLPQVEQLKS SIKELGSKYN SFVTDRNTAV EEALAKLDDE LKTKYQNKEL ALTDKYISDL
     QETKRQIELK HDQILAKELD TAQRRILLEA ENIIVQARIN TLSEFESIIS DKIDNERNGK
     LKNLDALAKR VEELENVQIK LFDNISNAEK LTNLKKTVSK INRLLISSND GVDAKTLINE
     VNKFKTYSKD LNNELISSVL LNLPNDKALS NGVLSQAQLL ARWDLLTPEL RSASLLPPNA
     GILGHLSSKL FSFFLLGKSG TPTSGNDIES VISRVHDNLL KNRLDDALEE VSSLKGWSRK
     LSEDWIVEAR KKLELQVLVG VLENEVSLL