ID   MIC60_LACTC             Reviewed;         527 AA.
AC   C5E325;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; OrderedLocusNames=KLTH0H09724g;
OS   Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS   (Yeast) (Kluyveromyces thermotolerans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=559295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284;
RX   PubMed=19525356; DOI=10.1101/gr.091546.109;
RG   The Genolevures Consortium;
RA   Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA   Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA   Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA   Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA   Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA   Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA   Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA   Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA   Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA   Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT   "Comparative genomics of protoploid Saccharomycetaceae.";
RL   Genome Res. 19:1696-1709(2009).
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; CU928180; CAR30436.1; -; Genomic_DNA.
DR   RefSeq; XP_002556298.1; XM_002556252.1.
DR   AlphaFoldDB; C5E325; -.
DR   SMR; C5E325; -.
DR   STRING; 559295.C5E325; -.
DR   EnsemblFungi; CAR30436; CAR30436; KLTH0H09724g.
DR   GeneID; 8294623; -.
DR   KEGG; lth:KLTH0H09724g; -.
DR   eggNOG; KOG1854; Eukaryota.
DR   HOGENOM; CLU_008024_2_0_1; -.
DR   InParanoid; C5E325; -.
DR   OMA; NRWNLLE; -.
DR   OrthoDB; 1540241at2759; -.
DR   Proteomes; UP000002036; Chromosome H.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 2.
DR   Pfam; PF09731; Mitofilin; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..24
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..527
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406657"
FT   TOPO_DOM        25..40
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..527
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   COILED          313..335
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   527 AA;  58100 MW;  A1F5038E7FA688AD CRC64;
     MLRSRATINC VKSGRAGRAL SRGMATFEGQ PAARPNRLSK LLVRVGLATV GFYVGGVTLS
     LNNDQFGELF CDNVPLAESL VEMYEEFRDE KMQASRMSLD ELKQKFGELG TKVDRIPNRG
     ADPALTSQAV AALPASKEVR LEDESLVKLR LPEVEQLGSC KRATPLVESV NAAVAAVNEQ
     SLLLPEDTYN AVHDAFTKLK SALQAINEDI RTNVAESVAV QYGQASKDLH ESFEIRAKSR
     EVELTQQFLN EFNAFKAQLE KHSSEELASA LKANEQALLA KQSNEVALLS MKQVEEFTKI
     LSEKLDQERQ GRLSKLEALN GSVQELAEAV DQVDTLVMKS EVLSQLSLLT TLLKNKLHAG
     DESSVKIDSE LARLKTLCDI LPGRPSKCCS KNPQLLDVVV SQLDSLASQQ LILSNEQLYN
     RWTLLQKDLS TSSLLPPNAG ILGHISAKIF GFFLFNKNGA PVDNDIDSVI ARVGQNLRLS
     KLDKAVEEVV ALKGWPRVLC DEWVQEARKK LEIETLIDAL DCEIRSS