ID   MIC60_PICGU             Reviewed;         528 AA.
AC   A5D9S8;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 2.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; ORFNames=PGUG_00033;
OS   Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS   / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX   NCBI_TaxID=294746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; CH408155; EDK35935.2; -; Genomic_DNA.
DR   RefSeq; XP_001486656.1; XM_001486606.1.
DR   AlphaFoldDB; A5D9S8; -.
DR   SMR; A5D9S8; -.
DR   STRING; 4929.XP_001486656.1; -.
DR   EnsemblFungi; EDK35935; EDK35935; PGUG_00033.
DR   GeneID; 5129562; -.
DR   KEGG; pgu:PGUG_00033; -.
DR   eggNOG; KOG1854; Eukaryota.
DR   HOGENOM; CLU_008024_2_0_1; -.
DR   InParanoid; A5D9S8; -.
DR   OMA; LQGWAKV; -.
DR   OrthoDB; 1540241at2759; -.
DR   Proteomes; UP000001997; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 1.
DR   Pfam; PF09731; Mitofilin; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..528
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406669"
FT   TOPO_DOM        ?..42
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..528
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REGION          136..164
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          199..349
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   528 AA;  58844 MW;  FC0F3DA25DDDB7EC CRC64;
     MLRARFSTSR SVLTKVVTPP PVVPPVLPPP VKPPKKSFSL PKFLFSATLI GSLAYGGTLY
     VATKNEKVMD YVIDYQPPFY EEILRMIETG SIDEIRDAWD SLRDRISNVD FSSSKSKIDK
     FANDLENRGE QLIQKTRQRW GQDTAPTPHE QLQKPVETTQ KSPESLPLLS YKGINESVHA
     TVASFNELIK SIDISGPGSN DLVKAIQDNV AKLSQKVDAL TASFDDELKK NLKVSQNELL
     SSYTKKELEL TENLLHQYNT ERAQLEKKLN ARLEQEIASA KEAISQAAVN AVTMVRIEQT
     KNFEKLVKDK VDGERSGRLA NLDKLSSRLD ELEQFAESLE QQIVANHNRS TINRSLAELR
     SLISSTEGTT PKSLAPYLGQ LIEAVRPIND ELIDATLKEI VPLLQHESSH SILSTSQLLA
     RWELLSPELR SASLLPPNAG LLGHFTSFVF SKLLLPVKGA KPNGKDIESV IGRVEANLVR
     NELDLAVEEA ASLKGWPRKL ADDWVVEARK RLEAEFLLGL IEGEVRSL