ID   MIC60_YEAS8             Reviewed;         539 AA.
AC   C8ZCI0;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=MICOS complex subunit MIC60;
DE   AltName: Full=Mitofilin;
DE   Flags: Precursor;
GN   Name=MIC60; ORFNames=EC1118_1K5_2740g;
OS   Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's
OS   yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=643680;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Lalvin EC1118 / Prise de mousse;
RX   PubMed=19805302; DOI=10.1073/pnas.0904673106;
RA   Novo M., Bigey F., Beyne E., Galeote V., Gavory F., Mallet S., Cambon B.,
RA   Legras J.-L., Wincker P., Casaregola S., Dequin S.;
RT   "Eukaryote-to-eukaryote gene transfer events revealed by the genome
RT   sequence of the wine yeast Saccharomyces cerevisiae EC1118.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:16333-16338(2009).
CC   -!- FUNCTION: Component of the MICOS complex, a large protein complex of
CC       the mitochondrial inner membrane that plays crucial roles in the
CC       maintenance of crista junctions, inner membrane architecture, and
CC       formation of contact sites to the outer membrane. Plays a role in
CC       keeping cristae membranes connected to the inner boundary membrane.
CC       Also promotes protein import via the mitochondrial intermembrane space
CC       assembly (MIA) pathway (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MICOS complex subunit Mic60 family.
CC       {ECO:0000305}.
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DR   EMBL; FN393077; CAY81096.1; -; Genomic_DNA.
DR   AlphaFoldDB; C8ZCI0; -.
DR   SMR; C8ZCI0; -.
DR   EnsemblFungi; CAY81096; CAY81096; EC1118_1K5_2740g.
DR   HOGENOM; CLU_008024_2_0_1; -.
DR   Proteomes; UP000000286; Chromosome XI, Scaffold EC1118_1K5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019133; Mt-IM_prot_Mitofilin.
DR   PANTHER; PTHR15415; PTHR15415; 2.
DR   Pfam; PF09731; Mitofilin; 3.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..16
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..539
FT                   /note="MICOS complex subunit MIC60"
FT                   /id="PRO_0000406683"
FT   TOPO_DOM        17..36
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..539
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   COILED          172..264
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   539 AA;  60913 MW;  AD67C0CFC10381C1 CRC64;
     MLRTTASRKI VLRRGLASIN TGTTVASKKA SHKFRNTFWT IALSATAFYA GGIIYSQKND
     KFGDFFSNNV PFAEDLLETY EHYHDRPTLF LEDSWDGLKA KSNDLLSGLT GSSQTRRSNR
     ENIEVKKILS LEPLNIETEN SDPQLKEIIG SLNDLINSLN DSNLSIPESE FNSIKKSNQN
     MLTNLSQLNE TLKEALSNYM IQRTSEVITE LNTQYENSKR EFEKNLQKNL LQEVDEFKEN
     LTKQKDKELE EKLKANEELL QAKHANGVGL LSITQVKEFN KIIKDKIEKE RNGRLAHLEE
     INSEVNDLSK SIDRSSKILS KNEALVQLTF QVDEIKSRIN NNNLPDVNID KELSRLKLLS
     NLLSTFNKKS CCDDGDCCSC KKGNKNEGKE GKISCKCKPK TNPPSLLSVA LDELESTCSG
     KKILSNEQIY NRWNLLADDF KTASLLPPNS GILGQLTAKV FSLFLFTKTG NPSNATDFDS
     VYARVGDNLR VSNLNDAVEE VVSLKGWPHK VCESWIEDAR RKLEVQRLVE ILDCEIRTL