ID   MICA_CLAMM              Reviewed;          68 AA.
AC   Q09T02;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Lantibiotic michiganin-A;
DE   Flags: Precursor;
GN   Name=micA {ECO:0000312|EMBL:ABE60717.1};
OS   Clavibacter michiganensis subsp. michiganensis.
OC   Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Clavibacter.
OX   NCBI_TaxID=33013;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ABE60717.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 48-68, FUNCTION,
RP   DEHYDRATION AT THR-55, LANTHIONINE CROSS-LINKS, AND MASS SPECTROMETRY.
RC   STRAIN=NCPPB 1468 / LMG3690 / ICMP 2541 {ECO:0000312|EMBL:ABE60717.1};
RX   PubMed=16957199; DOI=10.1128/aem.00639-06;
RA   Holtsmark I., Mantzilas D., Eijsink V.G., Brurberg M.B.;
RT   "Purification, characterization, and gene sequence of michiganin A, an
RT   actagardine-like lantibiotic produced by the tomato pathogen Clavibacter
RT   michiganensis subsp. michiganensis.";
RL   Appl. Environ. Microbiol. 72:5814-5821(2006).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC       active against S.michiganensis subspecies sepedonicus strain 2136
CC       (MIC=30 pmol/ml). The bactericidal activity of lantibiotics is based on
CC       depolarization of energized bacterial cytoplasmic membranes, initiated
CC       by the formation of aqueous transmembrane pores.
CC       {ECO:0000269|PubMed:16957199}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC       Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC       with cysteine. The 62-67 beta-methyllanthionine thioether bond is
CC       oxidized to a sulfoxide. This is followed by membrane translocation and
CC       cleavage of the modified precursor. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=2145; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16957199};
CC   -!- SIMILARITY: Belongs to the type B lantibiotic family. {ECO:0000255}.
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DR   EMBL; DQ458780; ABE60717.1; -; Genomic_DNA.
DR   RefSeq; WP_012038654.1; NZ_QODA01000011.1.
DR   AlphaFoldDB; Q09T02; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0046931; P:pore complex assembly; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Lantibiotic; Oxidation; Secreted; Thioether bond.
FT   PROPEP          1..47
FT                   /evidence="ECO:0000269|PubMed:16957199"
FT                   /id="PRO_0000283821"
FT   PEPTIDE         48..68
FT                   /note="Lantibiotic michiganin-A"
FT                   /id="PRO_5000141526"
FT   MOD_RES         55
FT                   /note="2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:16957199"
FT   CROSSLNK        49..54
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:16957199"
FT   CROSSLNK        57..65
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:16957199"
FT   CROSSLNK        62..67
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:16957199"
SQ   SEQUENCE   68 AA;  7572 MW;  0423E7DDA3856F61 CRC64;
     MNDILETETP VMVSPRWDML LDAGEDTSPS VQTQIDAEFR RVVSPYMSSS GWLCTLTIEC
     GTIICACR