MICU3_MOUSE
ID MICU3_MOUSE Reviewed; 523 AA.
AC Q9CTY5;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Calcium uptake protein 3, mitochondrial;
DE AltName: Full=EF-hand domain-containing family member A2;
GN Name=Micu3; Synonyms=Efha2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-258.
RC STRAIN=C57BL/6J; TISSUE=Hippocampus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=23409044; DOI=10.1371/journal.pone.0055785;
RA Plovanich M., Bogorad R.L., Sancak Y., Kamer K.J., Strittmatter L.,
RA Li A.A., Girgis H.S., Kuchimanchi S., De Groot J., Speciner L., Taneja N.,
RA Oshea J., Koteliansky V., Mootha V.K.;
RT "MICU2, a paralog of MICU1, resides within the mitochondrial uniporter
RT complex to regulate calcium handling.";
RL PLoS ONE 8:E55785-E55785(2013).
CC -!- FUNCTION: May play a role in mitochondrial calcium uptake.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}. Membrane
CC {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in skeletal muscle and
CC central nervous system. {ECO:0000269|PubMed:23409044}.
CC -!- MISCELLANEOUS: Binds calcium. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MICU1 family. MICU3 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB31674.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC102680; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC160537; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK019353; BAB31674.1; ALT_INIT; mRNA.
DR CCDS; CCDS52545.1; -.
DR RefSeq; NP_084386.1; NM_030110.1.
DR AlphaFoldDB; Q9CTY5; -.
DR SMR; Q9CTY5; -.
DR BioGRID; 219445; 4.
DR IntAct; Q9CTY5; 1.
DR MINT; Q9CTY5; -.
DR STRING; 10090.ENSMUSP00000070241; -.
DR iPTMnet; Q9CTY5; -.
DR PhosphoSitePlus; Q9CTY5; -.
DR SwissPalm; Q9CTY5; -.
DR MaxQB; Q9CTY5; -.
DR PaxDb; Q9CTY5; -.
DR PeptideAtlas; Q9CTY5; -.
DR PRIDE; Q9CTY5; -.
DR ProteomicsDB; 252563; -.
DR Antibodypedia; 8886; 59 antibodies from 17 providers.
DR Ensembl; ENSMUST00000068999; ENSMUSP00000070241; ENSMUSG00000039478.
DR GeneID; 78506; -.
DR KEGG; mmu:78506; -.
DR UCSC; uc009lmm.2; mouse.
DR CTD; 286097; -.
DR MGI; MGI:1925756; Micu3.
DR VEuPathDB; HostDB:ENSMUSG00000039478; -.
DR eggNOG; KOG2643; Eukaryota.
DR GeneTree; ENSGT00950000183079; -.
DR HOGENOM; CLU_027103_0_2_1; -.
DR InParanoid; Q9CTY5; -.
DR OMA; VQKYTTF; -.
DR OrthoDB; 707988at2759; -.
DR PhylomeDB; Q9CTY5; -.
DR TreeFam; TF320374; -.
DR Reactome; R-MMU-8949215; Mitochondrial calcium ion transport.
DR Reactome; R-MMU-8949664; Processing of SMDT1.
DR BioGRID-ORCS; 78506; 4 hits in 74 CRISPR screens.
DR PRO; PR:Q9CTY5; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CTY5; protein.
DR Bgee; ENSMUSG00000039478; Expressed in ventromedial nucleus of hypothalamus and 219 other tissues.
DR ExpressionAtlas; Q9CTY5; baseline and differential.
DR GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR039800; MICU1/2/3.
DR PANTHER; PTHR12294; PTHR12294; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 2.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Calcium; Membrane; Metal-binding; Mitochondrion; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..523
FT /note="Calcium uptake protein 3, mitochondrial"
FT /id="PRO_0000251231"
FT TRANSMEM 66..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 226..261
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 395..430
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 464..499
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 238
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 240
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 242
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 244
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 249
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 476
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 478
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 480
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 482
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 487
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 523 AA; 59810 MW; FD8A996423E6A146 CRC64;
MAALRRFLWP PPRLSPALAP QQPFLSPWGR PAGTAPGMSG RPFSCREEDE GAVAEAAWRR
RRWGELSIAA AAGGGLVGLV CYQLYGDPRA DPSELAAPEL EDPPRGRGLL PIPVAAAKET
VATGRAITED LDLYATSRER RFRLFASIEC EGQLFMTPYD FILAVTTDEP KFAKTWKSLS
KQELSQMLSE TPPVWKGSSK LFRNLKERGV ISYTEYLFLL CILTKPHAGF RIAFNMFDTD
GNEMVDKKEF LVLQEIFRKK NEKRETKGDE EKRAMLRLQL YGYHSPTNSV LKTDAGELVS
RSYWDTLRRS TSQALFSDLA ERADDITSLV ADTTLLVHFF GKKGKAELNF EDFYRFMDNL
QTEVLEIEFL SYSNGMNTIS EEDFAHILLR YTNVENTSVF LENVRYSISE EKGITFDEFR
SFFQFLNNLE DFAIALNMYN FASRSIGQDE FKRAVYVATG LKLSPHLVNT VFKIFDVDKD
DQLSYKEFIG IMKDRLHRGF RGYKTVQKYP TFKSCLKKEL HSR
