MID2_YEAST
ID MID2_YEAST Reviewed; 376 AA.
AC P36027; D6VYX3; P41944;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Cell wall integrity sensor MID2;
DE AltName: Full=Mating pheromone-induced death protein 2;
DE AltName: Full=Protein kinase A interference protein 1;
DE AltName: Full=Serine-rich multicopy suppressor protein 1;
DE Flags: Precursor;
GN Name=MID2; Synonyms=KAI1, SMS1; OrderedLocusNames=YLR332W;
GN ORFNames=L8543.19;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7828875; DOI=10.1016/0378-1119(94)90657-2;
RA Ono T., Suzuki T., Anraku Y., Iida H.;
RT "The MID2 gene encodes a putative integral membrane protein with a Ca(2+)-
RT binding domain and shows mating pheromone-stimulated expression in
RT Saccharomyces cerevisiae.";
RL Gene 151:203-208(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 70036;
RX PubMed=7999801; DOI=10.1016/0167-4781(94)00188-9;
RA Takeuchi J., Okada M., Toh-e A., Kikuchi Y.;
RT "The SMS1 gene encoding a serine-rich transmembrane protein suppresses the
RT temperature sensitivity of the htr1 disruptant in Saccharomyces
RT cerevisiae.";
RL Biochim. Biophys. Acta 1260:94-96(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-304.
RX PubMed=8355657; DOI=10.1007/bf00277063;
RA Daniel J.;
RT "Potentially rapid walking in cellular regulatory networks using the gene-
RT gene interference method in yeast.";
RL Mol. Gen. Genet. 240:245-257(1993).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=10348843; DOI=10.1128/jb.181.11.3330-3340.1999;
RA Ketela T., Green R., Bussey H.;
RT "Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and
RT upstream activator of the PKC1-MPK1 cell integrity pathway.";
RL J. Bacteriol. 181:3330-3340(1999).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX PubMed=10330137; DOI=10.1128/mcb.19.6.3969;
RA Rajavel M., Philip B., Buehrer B.M., Errede B., Levin D.E.;
RT "Mid2 is a putative sensor for cell integrity signaling in Saccharomyces
RT cerevisiae.";
RL Mol. Cell. Biol. 19:3969-3976(1999).
RN [8]
RP FUNCTION, AND GLYCOSYLATION.
RX PubMed=11113201; DOI=10.1128/mcb.21.1.271-280.2001;
RA Philip B., Levin D.E.;
RT "Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling
RT that act through Rom2, a guanine nucleotide exchange factor for Rho1.";
RL Mol. Cell. Biol. 21:271-280(2001).
RN [9]
RP INTERACTION WITH ZEO1.
RX PubMed=12949174; DOI=10.1099/mic.0.26471-0;
RA Green R., Lesage G., Sdicu A.-M., Menard P., Bussey H.;
RT "A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p,
RT and identification of a Mid2p-interacting protein, Zeo1p, that modulates
RT the PKC1-MPK1 cell integrity pathway.";
RL Microbiology 149:2487-2499(2003).
RN [10]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [11]
RP SUBCELLULAR LOCATION.
RX PubMed=13679573; DOI=10.1073/pnas.2033246100;
RA Shepard K.A., Gerber A.P., Jambhekar A., Takizawa P.A., Brown P.O.,
RA Herschlag D., DeRisi J.L., Vale R.D.;
RT "Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-
RT localized transcripts using DNA microarray analysis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11429-11434(2003).
RN [12]
RP FUNCTION.
RX PubMed=16087742; DOI=10.1128/ec.4.8.1375-1386.2005;
RA Claret S., Gatti X., Doignon F., Thoraval D., Crouzet M.;
RT "The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are
RT required at low pH for protein kinase C pathway activation and cell
RT survival in Saccharomyces cerevisiae.";
RL Eukaryot. Cell 4:1375-1386(2005).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260; SER-327; SER-329 AND
RP SER-355, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Cell wall stress sensor. Involved in activation of a response
CC that includes both stress-related increased chitin synthesis and the
CC MPK1 mitogen-activated protein kinase cell integrity pathway. High-copy
CC activator of the SKN7 transcription factor. Required during induction
CC of MPK1 tyrosine phosphorylation under a variety of stress conditions.
CC Required for PKC pathway activation under low pH conditions. Required
CC for cell integrity signaling during pheromone-induced morphogenesis.
CC Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward
CC RHO1 in cell wall integrity signaling. {ECO:0000269|PubMed:10330137,
CC ECO:0000269|PubMed:10348843, ECO:0000269|PubMed:11113201,
CC ECO:0000269|PubMed:16087742}.
CC -!- SUBUNIT: Interacts with ZEO1. {ECO:0000269|PubMed:12949174}.
CC -!- INTERACTION:
CC P36027; P25623: SYP1; NbExp=2; IntAct=EBI-10901, EBI-21900;
CC P36027; Q08245: ZEO1; NbExp=3; IntAct=EBI-10901, EBI-32089;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10330137,
CC ECO:0000269|PubMed:10348843, ECO:0000269|PubMed:13679573,
CC ECO:0000269|PubMed:14562095}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:10330137, ECO:0000269|PubMed:10348843,
CC ECO:0000269|PubMed:13679573, ECO:0000269|PubMed:14562095}.
CC Note=Localizes uniformly around the cell periphery. Also localized to
CC the mother-bud junction.
CC -!- PTM: O-mannosylated by PMT2.
CC -!- SIMILARITY: Belongs to the MID2 like cell wall stress sensor family.
CC {ECO:0000305}.
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DR EMBL; D29945; BAA06220.1; -; Genomic_DNA.
DR EMBL; D29964; BAA06230.1; -; Genomic_DNA.
DR EMBL; U20618; AAB64527.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09639.1; -; Genomic_DNA.
DR PIR; S52137; S52137.
DR RefSeq; NP_013436.1; NM_001182221.1.
DR AlphaFoldDB; P36027; -.
DR BioGRID; 31595; 253.
DR DIP; DIP-4676N; -.
DR IntAct; P36027; 52.
DR MINT; P36027; -.
DR STRING; 4932.YLR332W; -.
DR iPTMnet; P36027; -.
DR MaxQB; P36027; -.
DR PaxDb; P36027; -.
DR PRIDE; P36027; -.
DR EnsemblFungi; YLR332W_mRNA; YLR332W; YLR332W.
DR GeneID; 851042; -.
DR KEGG; sce:YLR332W; -.
DR SGD; S000004324; MID2.
DR VEuPathDB; FungiDB:YLR332W; -.
DR eggNOG; ENOG502S35T; Eukaryota.
DR GeneTree; ENSGT00940000176476; -.
DR HOGENOM; CLU_056209_0_0_1; -.
DR InParanoid; P36027; -.
DR OMA; MLDEEKY; -.
DR BioCyc; YEAST:G3O-32412-MON; -.
DR PRO; PR:P36027; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P36027; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:SGD.
DR GO; GO:0043332; C:mating projection tip; HDA:SGD.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IMP:SGD.
DR GO; GO:0030242; P:autophagy of peroxisome; IMP:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR GO; GO:0036498; P:IRE1-mediated unfolded protein response; IMP:SGD.
DR GO; GO:0010447; P:response to acidic pH; IMP:SGD.
DR GO; GO:0006970; P:response to osmotic stress; IGI:SGD.
DR InterPro; IPR007567; Mid2_dom.
DR Pfam; PF04478; Mid2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Signal; Stress response; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..376
FT /note="Cell wall integrity sensor MID2"
FT /id="PRO_0000096485"
FT TOPO_DOM 26..224
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 59..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 126..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 260
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 327
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 329
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 303..304
FT /note="GG -> VV (in Ref. 5; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 39146 MW; 3612E260BC1B04A2 CRC64;
MLSFTTKNSF RLLLLILSCI STIRAQFFVQ SSSSNSSAVS TARSSVSRVS SSSSILSSSM
VSSSSADSSS LTSSTSSRSL VSHTSSSTSI ASISFTSFSF SSDSSTSSSS SASSDSSSSS
SFSISSTSAT SESSTSSTQT STSSSSSLSS TPSSSSSPST ITSAPSTSST PSTTAYNQGS
TITSIINGKT ILSNHYTTVT YTPSATADSS NKSKSSGLSK KNRNIVIGCV VGIGVPLILV
ILALIYMFCI QSSRTDFIDS DGKVVTAYRA NKFTKWWYML LGKKVSDEYH SDSPLGGSAS
SAGGLDLDEA DDVMEQSSLF DVRIRDSDSV LPNANTADHN NTNSGGEPIN SSVASNDIIE
EKFYDEQGNE LSPRNY