ID   MID49_PONAB             Reviewed;         454 AA.
AC   Q5RA76; Q5RC08;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Mitochondrial dynamics protein MID49;
DE   AltName: Full=Mitochondrial dynamics protein of 49 kDa homolog;
DE   AltName: Full=Mitochondrial elongation factor 2;
DE   AltName: Full=Smith-Magenis syndrome chromosomal region candidate gene 7 protein homolog;
GN   Name=MIEF2; Synonyms=MID49, SMCR7;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex, and Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial outer membrane protein which regulates
CC       mitochondrial organization (By similarity). It is required for
CC       mitochondrial fission and promotes the recruitment and association of
CC       the fission mediator dynamin-related protein 1 (DNM1L) to the
CC       mitochondrial surface independently of the mitochondrial fission FIS1
CC       and MFF proteins (By similarity). Regulates DNM1L GTPase activity (By
CC       similarity). {ECO:0000250|UniProtKB:Q96C03}.
CC   -!- SUBUNIT: Interacts with DNM1L. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass
CC       membrane protein. Note=Colocalizes with DNM1L at mitochondrial
CC       membrane. Forms foci and rings around mitochondria (By similarity).
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Does not bind ADP or other nucleotides, in contrast to
CC       MIEF1. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MID49/MID51 family. {ECO:0000305}.
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DR   EMBL; CR858474; CAH90702.1; -; mRNA.
DR   EMBL; CR859143; CAH91334.1; -; mRNA.
DR   RefSeq; NP_001125789.1; NM_001132317.1.
DR   AlphaFoldDB; Q5RA76; -.
DR   SMR; Q5RA76; -.
DR   STRING; 9601.ENSPPYP00000009052; -.
DR   GeneID; 100172717; -.
DR   KEGG; pon:100172717; -.
DR   CTD; 125170; -.
DR   eggNOG; ENOG502QPJX; Eukaryota.
DR   InParanoid; Q5RA76; -.
DR   OrthoDB; 515815at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0003374; P:dynamin family protein polymerization involved in mitochondrial fission; ISS:UniProtKB.
DR   GO; GO:0007005; P:mitochondrion organization; ISS:UniProtKB.
DR   GO; GO:0090141; P:positive regulation of mitochondrial fission; ISS:UniProtKB.
DR   GO; GO:0090314; P:positive regulation of protein targeting to membrane; ISS:UniProtKB.
DR   GO; GO:0010821; P:regulation of mitochondrion organization; ISS:UniProtKB.
DR   InterPro; IPR024810; Mab-21_dom.
DR   InterPro; IPR045909; MID49/MID51.
DR   PANTHER; PTHR16451; PTHR16451; 1.
DR   Pfam; PF03281; Mab-21; 1.
DR   SMART; SM01265; Mab-21; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion outer membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..454
FT                   /note="Mitochondrial dynamics protein MID49"
FT                   /id="PRO_0000310447"
FT   TOPO_DOM        1..22
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        44..454
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          76..119
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        197
FT                   /note="L -> Q (in Ref. 1; CAH90702)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        365
FT                   /note="R -> H (in Ref. 1; CAH90702)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   454 AA;  49270 MW;  9BF94A0A155E053F CRC64;
     MAEFSQKRGK RRGDEGLGSM VDFLLANARL VLGVGGAAVL GIATLAVKRF IDRATSPRDE
     DDTKADSWKE LSLLKATPHL QPRPPPAALS QPVLPLAPSS SAPEGPAKSD PEVTPQLSSP
     APLCLTLQER LLAFERDRVT IPAAQVALAK QLAGDIALEL QAYFQSKFPE LPFGAFVPGG
     PLYDGLQAGA ADHVRLLVPL VLEPGLWSLV PGVDTVARDP RCWAVRRTQL EFCPRGSSPW
     DRFLVGGYLS SRVLLELLRK VLAASVNWPA IGSLLGCLIR PSMASEELLL EVQHERLELT
     VAVLVAVPGV DADDRLLLAW PLEGLAGNLW LQDLYPVEAA RLRALDDRDA GTRRRLLLLL
     CAVCRGCSAL GQLGRGHLTQ VVLRLGEDNV DWTEEALGER FLQALELLIG SLEQASLPCH
     FNPSVNLFSN LREEEIDDIG YALYSGLQEP EGLL