ID   MIDNB_XENLA             Reviewed;         451 AA.
AC   Q7ZWX9; Q3KPP8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Midnolin-B;
DE   AltName: Full=Midbrain nucleolar protein B;
GN   Name=midn-b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Embryo, and Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Facilitates ubiquitin-independent proteasomal degradation of
CC       polycomb protein CBX4. Plays a role in inhibiting the activity of
CC       glucokinase GCK and both glucose-induced and basal insulin secretion.
CC       {ECO:0000250|UniProtKB:D4AE48, ECO:0000250|UniProtKB:Q3TPJ7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q3TPJ7}.
CC       Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q3TPJ7}. Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q3TPJ7}. Note=Detected in the nucleus and
CC       nucleolus with no expression in the cytoplasm. However, a later study
CC       finds expression in the nucleus and cytoplasm with no expression in the
CC       nucleolus. {ECO:0000250|UniProtKB:Q3TPJ7}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7ZWX9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7ZWX9-2; Sequence=VSP_025554;
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DR   EMBL; BC046658; AAH46658.1; -; mRNA.
DR   EMBL; BC106621; AAI06622.1; -; mRNA.
DR   RefSeq; NP_001079641.1; NM_001086172.1. [Q7ZWX9-1]
DR   AlphaFoldDB; Q7ZWX9; -.
DR   SMR; Q7ZWX9; -.
DR   DNASU; 379328; -.
DR   GeneID; 379328; -.
DR   KEGG; xla:379328; -.
DR   CTD; 379328; -.
DR   Xenbase; XB-GENE-6256089; midn.S.
DR   OMA; PEANCAK; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 379328; Expressed in gastrula and 19 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR039336; Midnolin.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR23010; PTHR23010; 1.
DR   Pfam; PF00240; ubiquitin; 1.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..451
FT                   /note="Midnolin-B"
FT                   /id="PRO_0000287540"
FT   DOMAIN          20..94
FT                   /note="Ubiquitin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT   REGION          187..254
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          331..372
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          388..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..223
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        234..254
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        331..363
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        408..427
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         233
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_025554"
SQ   SEQUENCE   451 AA;  49213 MW;  842C70C2E740CF95 CRC64;
     MEQQPSVPRS CTNVARETPM NLNIQSTTGT RYELSVPLNE TVDGLKRRIS QRLKVPKERL
     TLLHRETRLS SGKLQDLGIS DGSRLTLLPS VEAGLMSQMS RPEQSVMQAL ESLTETQVND
     FLSGRSPLTL ALRVGDHMMF VQLQLAAQQS GGSHLQHRHV ITRGADAGAP PQYRTLHTST
     SALSHLASCT PGSTPPTTLS PTSSTHCDAP HSSPLTTSVF RSHGEGVSPC AEQVPCSTRG
     TEGTSSSPSS RSRKPGAIIE SFVNHAPGVF SGTFSGTLHP HCQDGAGRPR RDIGTILQIL
     NDLLSATRHY QGMPPSLTTL RCHTQCASQA RNAKATSPQS TGPQQTTHPV GHCQAQTRTC
     KPSGDRLRQT ENRATRCKVE RLQLLMHQKR LRRKARRDSR APYHWMPTRK SSRTSSNSST
     SSGEGSLEID FEDSLWKPDV KAELNSEFVV A