MIDN_DANRE
ID MIDN_DANRE Reviewed; 509 AA.
AC Q6NYU6;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Midnolin;
DE AltName: Full=Midbrain nucleolar protein;
GN Name=midn; ORFNames=ch211-79c14.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Facilitates ubiquitin-independent proteasomal degradation of
CC polycomb protein CBX4. Plays a role in inhibiting the activity of
CC glucokinase GCK and both glucose-induced and basal insulin secretion.
CC {ECO:0000250|UniProtKB:D4AE48, ECO:0000250|UniProtKB:Q3TPJ7}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q3TPJ7}.
CC Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q3TPJ7}. Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q3TPJ7}. Note=Detected in the nucleus and
CC nucleolus with no expression in the cytoplasm. However, a later study
CC finds expression in the nucleus and cytoplasm with no expression in the
CC nucleolus. {ECO:0000250|UniProtKB:Q3TPJ7}.
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DR EMBL; BX649276; CAK04988.1; -; Genomic_DNA.
DR EMBL; BC066456; AAH66456.1; -; mRNA.
DR RefSeq; NP_996935.1; NM_207052.1.
DR AlphaFoldDB; Q6NYU6; -.
DR SMR; Q6NYU6; -.
DR STRING; 7955.ENSDARP00000022102; -.
DR PaxDb; Q6NYU6; -.
DR Ensembl; ENSDART00000003929; ENSDARP00000022102; ENSDARG00000018524.
DR Ensembl; ENSDART00000167466; ENSDARP00000140760; ENSDARG00000018524.
DR GeneID; 323713; -.
DR KEGG; dre:323713; -.
DR CTD; 90007; -.
DR ZFIN; ZDB-GENE-030131-2433; midn.
DR eggNOG; ENOG502QTDX; Eukaryota.
DR GeneTree; ENSGT00510000049027; -.
DR InParanoid; Q6NYU6; -.
DR PhylomeDB; Q6NYU6; -.
DR TreeFam; TF329735; -.
DR PRO; PR:Q6NYU6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 22.
DR Bgee; ENSDARG00000018524; Expressed in somite and 49 other tissues.
DR ExpressionAtlas; Q6NYU6; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR InterPro; IPR039336; Midnolin.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR23010; PTHR23010; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..509
FT /note="Midnolin"
FT /id="PRO_0000287538"
FT DOMAIN 28..102
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 172..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 370..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 448..485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..192
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..230
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..389
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 390..404
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..485
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 509 AA; 54873 MW; A8E6C4DA1CAF4D78 CRC64;
MDQHPSARSC SSRGAAPSCE SVSGEPPMNL YIHSTTGTRF ELSLPAEETV EGLKRRLSQR
LKVPKERLAL LHKETRLSSG KLQDLGITDG SKLTLVPTVE AGLMSQASRP EQSVMQALES
LTETQVSDFL SGRSPLTLAL RVGDHMMFVQ LQLAAQPSGG PQLQHRHLIS RGSSEGTTGL
SHGASGSASG MARVSHNPHP HHPHQHPHHP NTTLPSNPAA FPPSPSIPSI PPMYSTSASG
HCSPPPQPSQ LPGSFLHSQQ PSSACAPSPS SPSPAASCPE ASCSAKTSGN CNTPLRSRKP
GAIIESFVNH APGVFSGTFS GTLHPNCQDS TGRPRRDIGT ILQILNDLLS ATRHYQGMPP
SLTQLRYQTQ CTSPNSPAPS PPPSPPHTTG LTGLPTTVPS ETQPTLHPLV QCQSQIRMCK
PPGDRLRQTE NRATRCKVER LQLLMQQKRL RRKARRDSRA PYHWLPNRKA GRSNSNSSMS
SEGSLDLDFE DSVWKPDVKA DMKSEFIMA
