MIDN_HUMAN
ID MIDN_HUMAN Reviewed; 468 AA.
AC Q504T8; Q96BW8;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Midnolin;
DE AltName: Full=Midbrain nucleolar protein;
GN Name=MIDN;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP INTERACTION WITH GCK, AND SUBCELLULAR LOCATION.
RX PubMed=24187134; DOI=10.1074/jbc.m113.526632;
RA Hofmeister-Brix A., Kollmann K., Langer S., Schultz J., Lenzen S.,
RA Baltrusch S.;
RT "Identification of the ubiquitin-like domain of midnolin as a new
RT glucokinase interaction partner.";
RL J. Biol. Chem. 288:35824-35839(2013).
CC -!- FUNCTION: Facilitates ubiquitin-independent proteasomal degradation of
CC polycomb protein CBX4. Plays a role in inhibiting the activity of
CC glucokinase GCK and both glucose-induced and basal insulin secretion.
CC {ECO:0000250|UniProtKB:D4AE48, ECO:0000250|UniProtKB:Q3TPJ7}.
CC -!- SUBUNIT: Interacts with GCK; the interaction occurs preferentially at
CC low glucose levels. {ECO:0000269|PubMed:24187134}.
CC -!- INTERACTION:
CC Q504T8; Q8IZR5-2: CMTM4; NbExp=3; IntAct=EBI-7153979, EBI-17278014;
CC Q504T8; O76011: KRT34; NbExp=3; IntAct=EBI-7153979, EBI-1047093;
CC Q504T8; Q9H8W4: PLEKHF2; NbExp=3; IntAct=EBI-7153979, EBI-742388;
CC Q504T8; O43761: SYNGR3; NbExp=3; IntAct=EBI-7153979, EBI-11321949;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q3TPJ7}. Nucleus {ECO:0000269|PubMed:24187134}.
CC Cytoplasm, cytosol {ECO:0000269|PubMed:24187134}. Note=Detected in the
CC nucleus and nucleolus with no expression in the cytoplasm (By
CC similarity). However, a later study finds expression in the nucleus and
CC cytoplasm with no expression in the nucleolus (PubMed:24187134).
CC {ECO:0000250|UniProtKB:Q3TPJ7, ECO:0000269|PubMed:24187134}.
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DR EMBL; BC015089; AAH15089.2; -; mRNA.
DR EMBL; BC094778; AAH94778.1; -; mRNA.
DR CCDS; CCDS32864.1; -.
DR RefSeq; NP_796375.3; NM_177401.4.
DR AlphaFoldDB; Q504T8; -.
DR SMR; Q504T8; -.
DR BioGRID; 124652; 31.
DR IntAct; Q504T8; 16.
DR MINT; Q504T8; -.
DR STRING; 9606.ENSP00000300952; -.
DR iPTMnet; Q504T8; -.
DR PhosphoSitePlus; Q504T8; -.
DR BioMuta; MIDN; -.
DR DMDM; 296437369; -.
DR jPOST; Q504T8; -.
DR MassIVE; Q504T8; -.
DR PaxDb; Q504T8; -.
DR PeptideAtlas; Q504T8; -.
DR PRIDE; Q504T8; -.
DR ProteomicsDB; 62403; -.
DR Antibodypedia; 22621; 128 antibodies from 18 providers.
DR DNASU; 90007; -.
DR Ensembl; ENST00000300952.7; ENSP00000300952.2; ENSG00000167470.14.
DR Ensembl; ENST00000591446.7; ENSP00000467679.1; ENSG00000167470.14.
DR GeneID; 90007; -.
DR KEGG; hsa:90007; -.
DR UCSC; uc002lrp.4; human.
DR CTD; 90007; -.
DR DisGeNET; 90007; -.
DR GeneCards; MIDN; -.
DR HGNC; HGNC:16298; MIDN.
DR HPA; ENSG00000167470; Low tissue specificity.
DR MIM; 606700; gene.
DR neXtProt; NX_Q504T8; -.
DR OpenTargets; ENSG00000167470; -.
DR PharmGKB; PA30818; -.
DR VEuPathDB; HostDB:ENSG00000167470; -.
DR eggNOG; ENOG502QTDX; Eukaryota.
DR GeneTree; ENSGT00510000049027; -.
DR HOGENOM; CLU_029882_0_0_1; -.
DR InParanoid; Q504T8; -.
DR OMA; EANCSTN; -.
DR OrthoDB; 1159802at2759; -.
DR PhylomeDB; Q504T8; -.
DR TreeFam; TF329735; -.
DR PathwayCommons; Q504T8; -.
DR SignaLink; Q504T8; -.
DR BioGRID-ORCS; 90007; 49 hits in 1085 CRISPR screens.
DR ChiTaRS; MIDN; human.
DR GenomeRNAi; 90007; -.
DR Pharos; Q504T8; Tbio.
DR PRO; PR:Q504T8; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q504T8; protein.
DR Bgee; ENSG00000167470; Expressed in oviduct epithelium and 178 other tissues.
DR ExpressionAtlas; Q504T8; baseline and differential.
DR Genevisible; Q504T8; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019900; F:kinase binding; ISS:UniProtKB.
DR GO; GO:0033132; P:negative regulation of glucokinase activity; ISS:UniProtKB.
DR GO; GO:0046676; P:negative regulation of insulin secretion; ISS:UniProtKB.
DR InterPro; IPR039336; Midnolin.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR23010; PTHR23010; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..468
FT /note="Midnolin"
FT /id="PRO_0000287536"
FT DOMAIN 31..105
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT REGION 182..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 404..447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..438
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 457
FT /note="V -> A (in Ref. 1; AAH94778)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 468 AA; 49213 MW; E4086F3B6E16C4C2 CRC64;
MEPQPGGARS CRRGAPGGAC ELGPAAEAAP MSLAIHSTTG TRYDLAVPPD ETVEGLRKRL
SQRLKVPKER LALLHKDTRL SSGKLQEFGV GDGSKLTLVP TVEAGLMSQA SRPEQSVMQA
LESLTETQVS DFLSGRSPLT LALRVGDHMM FVQLQLAAQH APLQHRHVLA AAAAAAAARG
DPSIASPVSS PCRPVSSAAR VPPVPTSPSP ASPSPITAGS FRSHAASTTC PEQMDCSPTA
SSSASPGAST TSTPGASPAP RSRKPGAVIE SFVNHAPGVF SGTFSGTLHP NCQDSSGRPR
RDIGTILQIL NDLLSATRHY QGMPPSLAQL RCHAQCSPAS PAPDLAPRTT SCEKLTAAPS
ASLLQGQSQI RMCKPPGDRL RQTENRATRC KVERLQLLLQ QKRLRRKARR DARGPYHWSP
SRKAGRSDSS SSGGGGSPSE ASGLGLDFED SVWKPEVNPD IKSEFVVA
