ID   MIDN_XENTR              Reviewed;         453 AA.
AC   Q5EB28;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Midnolin;
DE   AltName: Full=Midbrain nucleolar protein;
GN   Name=midn; ORFNames=TNeu058f14.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Facilitates ubiquitin-independent proteasomal degradation of
CC       polycomb protein CBX4. Plays a role in inhibiting the activity of
CC       glucokinase GCK and both glucose-induced and basal insulin secretion.
CC       {ECO:0000250|UniProtKB:D4AE48, ECO:0000250|UniProtKB:Q3TPJ7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q3TPJ7}.
CC       Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q3TPJ7}. Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q3TPJ7}. Note=Detected in the nucleus and
CC       nucleolus with no expression in the cytoplasm. However, a later study
CC       finds expression in the nucleus and cytoplasm with no expression in the
CC       nucleolus. {ECO:0000250|UniProtKB:Q3TPJ7}.
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DR   EMBL; CR760184; CAJ82671.1; -; mRNA.
DR   EMBL; BC090118; AAH90118.1; -; mRNA.
DR   RefSeq; NP_001015832.1; NM_001015832.1.
DR   AlphaFoldDB; Q5EB28; -.
DR   SMR; Q5EB28; -.
DR   STRING; 8364.ENSXETP00000021557; -.
DR   PaxDb; Q5EB28; -.
DR   DNASU; 549445; -.
DR   GeneID; 549445; -.
DR   KEGG; xtr:549445; -.
DR   CTD; 90007; -.
DR   Xenbase; XB-GENE-1005232; midn.
DR   eggNOG; ENOG502QTDX; Eukaryota.
DR   HOGENOM; CLU_029882_0_0_1; -.
DR   InParanoid; Q5EB28; -.
DR   OMA; EANCSTN; -.
DR   OrthoDB; 1159802at2759; -.
DR   PhylomeDB; Q5EB28; -.
DR   TreeFam; TF329735; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000003282; Expressed in gastrula and 12 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   InterPro; IPR039336; Midnolin.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR23010; PTHR23010; 1.
DR   Pfam; PF00240; ubiquitin; 1.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..453
FT                   /note="Midnolin"
FT                   /id="PRO_0000287541"
FT   DOMAIN          20..94
FT                   /note="Ubiquitin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT   REGION          185..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          231..256
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          330..374
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          390..434
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        236..256
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        330..365
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        410..429
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   453 AA;  49484 MW;  D0F2FDB1FA50CDE4 CRC64;
     MEQQPSVPRS CTNVARETPM NLNIQSTTGT RYELSVPPDE TVDGLKRRIS QRLKVPKERL
     TLLHRETRLS SGKLQDLGIS DGSRLTLLPS VEAGLMSQMS RPEQSVMQAL ESLTETQVND
     FLSGRSPLTL ALRVGDHMMF VQLQLAAQQS GASHLQHRHV ITRGAEAGAS PQYRTLHTST
     SALSHLASCT PGSTPPTTLS PTASTHRDGP HSSPLTTSVF RSHGEGVAVS PCAEQVPCSS
     RGTEGTSSSA SSRSRKPGAI IESFVNHAPG VFSGTFSGTL HPHCQDSAGR PRRDIGTILQ
     ILNDLLSATR HYQGMPPSLT TLRCHTQCAS QARNPKATSP QSSEPQQTTH PVGHCQAQTR
     TCKPSGDRLR QTENRATRCK VERLQLLMHQ KRLRRKARRD SRAPYHWMPT RKSSRTSSNS
     STSSGEGSLE IDFEDSLWKP DVKAELNSEF VVA