ID   MIER2_HUMAN             Reviewed;         545 AA.
AC   Q8N344; Q9ULM7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=Mesoderm induction early response protein 2;
DE            Short=Mi-er2;
GN   Name=MIER2; Synonyms=KIAA1193;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-545.
RC   TISSUE=Brain;
RX   PubMed=10574462; DOI=10.1093/dnares/6.5.337;
RA   Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XV. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 6:337-345(1999).
RN   [3]
RP   SEQUENCE REVISION.
RA   Ohara O., Nagase T., Kikuno R.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION IN A COMPLEX WITH CDYL; MIER1; HDAC1 AND HDAC2.
RX   PubMed=19061646; DOI=10.1016/j.molcel.2008.10.025;
RA   Mulligan P., Westbrook T.F., Ottinger M., Pavlova N., Chang B., Macia E.,
RA   Shi Y.J., Barretina J., Liu J., Howley P.M., Elledge S.J., Shi Y.;
RT   "CDYL bridges REST and histone methyltransferases for gene repression and
RT   suppression of cellular transformation.";
RL   Mol. Cell 32:718-726(2008).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [6]
RP   VARIANT TRP-289.
RX   PubMed=27400125; DOI=10.1172/jci85794;
RA   Martin-Almedina S., Martinez-Corral I., Holdhus R., Vicente A., Fotiou E.,
RA   Lin S., Petersen K., Simpson M.A., Hoischen A., Gilissen C., Jeffery H.,
RA   Atton G., Karapouliou C., Brice G., Gordon K., Wiseman J.W., Wedin M.,
RA   Rockson S.G., Jeffery S., Mortimer P.S., Snyder M.P., Berland S.,
RA   Mansour S., Makinen T., Ostergaard P.;
RT   "EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-
RT   related hydrops fetalis.";
RL   J. Clin. Invest. 126:3080-3088(2016).
CC   -!- FUNCTION: Transcriptional repressor. {ECO:0000250}.
CC   -!- SUBUNIT: Part of a complex containing at least CDYL, MIER1, MIER2,
CC       HDAC1 and HDAC2. {ECO:0000269|PubMed:19061646}.
CC   -!- INTERACTION:
CC       Q8N344; Q14005-2: IL16; NbExp=5; IntAct=EBI-3504938, EBI-17178971;
CC       Q8N344; Q96EZ8: MCRS1; NbExp=5; IntAct=EBI-3504938, EBI-348259;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00512,
CC       ECO:0000255|PROSITE-ProRule:PRU00624}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA86507.2; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR   EMBL; BC028203; AAH28203.2; -; mRNA.
DR   EMBL; AB033019; BAA86507.2; ALT_SEQ; mRNA.
DR   CCDS; CCDS32855.1; -.
DR   RefSeq; NP_060020.1; NM_017550.2.
DR   AlphaFoldDB; Q8N344; -.
DR   BioGRID; 120019; 35.
DR   IntAct; Q8N344; 29.
DR   STRING; 9606.ENSP00000264819; -.
DR   iPTMnet; Q8N344; -.
DR   PhosphoSitePlus; Q8N344; -.
DR   BioMuta; MIER2; -.
DR   DMDM; 74750947; -.
DR   EPD; Q8N344; -.
DR   jPOST; Q8N344; -.
DR   MassIVE; Q8N344; -.
DR   MaxQB; Q8N344; -.
DR   PaxDb; Q8N344; -.
DR   PeptideAtlas; Q8N344; -.
DR   PRIDE; Q8N344; -.
DR   ProteomicsDB; 71762; -.
DR   Antibodypedia; 42088; 252 antibodies from 24 providers.
DR   DNASU; 54531; -.
DR   Ensembl; ENST00000264819.7; ENSP00000264819.3; ENSG00000105556.12.
DR   GeneID; 54531; -.
DR   KEGG; hsa:54531; -.
DR   MANE-Select; ENST00000264819.7; ENSP00000264819.3; NM_017550.3; NP_060020.1.
DR   UCSC; uc002lok.1; human.
DR   CTD; 54531; -.
DR   DisGeNET; 54531; -.
DR   GeneCards; MIER2; -.
DR   HGNC; HGNC:29210; MIER2.
DR   HPA; ENSG00000105556; Low tissue specificity.
DR   neXtProt; NX_Q8N344; -.
DR   OpenTargets; ENSG00000105556; -.
DR   PharmGKB; PA134925804; -.
DR   VEuPathDB; HostDB:ENSG00000105556; -.
DR   eggNOG; KOG4329; Eukaryota.
DR   GeneTree; ENSGT01030000234573; -.
DR   HOGENOM; CLU_027202_3_1_1; -.
DR   InParanoid; Q8N344; -.
DR   OMA; HRQKAYE; -.
DR   OrthoDB; 1062529at2759; -.
DR   PhylomeDB; Q8N344; -.
DR   TreeFam; TF106453; -.
DR   PathwayCommons; Q8N344; -.
DR   SignaLink; Q8N344; -.
DR   BioGRID-ORCS; 54531; 9 hits in 1074 CRISPR screens.
DR   ChiTaRS; MIER2; human.
DR   GenomeRNAi; 54531; -.
DR   Pharos; Q8N344; Tbio.
DR   PRO; PR:Q8N344; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q8N344; protein.
DR   Bgee; ENSG00000105556; Expressed in apex of heart and 139 other tissues.
DR   ExpressionAtlas; Q8N344; baseline and differential.
DR   Genevisible; Q8N344; HS.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; IDA:UniProtKB.
DR   GO; GO:0042826; F:histone deacetylase binding; IPI:UniProtKB.
DR   GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR   GO; GO:0016575; P:histone deacetylation; IDA:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR000949; ELM2_dom.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR040138; MIER/MTA.
DR   InterPro; IPR001005; SANT/Myb.
DR   InterPro; IPR017884; SANT_dom.
DR   PANTHER; PTHR10865; PTHR10865; 1.
DR   Pfam; PF01448; ELM2; 1.
DR   SMART; SM01189; ELM2; 1.
DR   SMART; SM00717; SANT; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS51156; ELM2; 1.
DR   PROSITE; PS51293; SANT; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..545
FT                   /note="Mesoderm induction early response protein 2"
FT                   /id="PRO_0000313678"
FT   DOMAIN          195..292
FT                   /note="ELM2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00512"
FT   DOMAIN          297..349
FT                   /note="SANT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT   REGION          100..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          364..464
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        143..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        166..180
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         11
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VARIANT         68
FT                   /note="D -> N (in dbSNP:rs7507468)"
FT                   /id="VAR_037695"
FT   VARIANT         104
FT                   /note="D -> N (in dbSNP:rs10421231)"
FT                   /id="VAR_037696"
FT   VARIANT         289
FT                   /note="R -> W (unknown pathological significance;
FT                   dbSNP:rs148482834)"
FT                   /evidence="ECO:0000269|PubMed:27400125"
FT                   /id="VAR_078065"
FT   VARIANT         464
FT                   /note="P -> S (in dbSNP:rs34129568)"
FT                   /id="VAR_037697"
FT   VARIANT         485
FT                   /note="S -> G (in dbSNP:rs35042658)"
FT                   /id="VAR_037698"
SQ   SEQUENCE   545 AA;  59944 MW;  891526F8094F18D8 CRC64;
     MAEASSLGRQ SPRVVSCLEH SLCPGEPGLQ TTAVVSMGSG DHQFNLAEIL SQNYSVRGEC
     EEASRCPDKP KEELEKDFIS QSNDMPFDEL LALYGYEASD PISDRESEGG DVAPNLPDMT
     LDKEQIAKDL LSGEEEEETQ SSADDLTPSV TSHEASDLFP NRSGSRFLAD EDREPGSSAS
     SDTEEDSLPA NKCKKEIMVG PQFQADLSNL HLNRHCEKIY ENEDQLLWDP SVLPEREVEE
     FLYRAVKRRW HEMAGPQLPE GEAVKDSEQA LYELVKCNFN VEEALRRLRF NVKVIRDGLC
     AWSEEECRNF EHGFRVHGKN FHLIQANKVR TRSVGECVEY YYLWKKSERY DYFAQQTRLG
     RRKYVPSGTT DADQDLDGSD PDGPGRPRPE QDTLTGMRTD PLSVDGTAGG LDEPGVASDG
     LPSSEPGPCS FQQLDESPAV PLSHRPPALA DPASYQPAVT APEPDASPRL AVDFALPKEL
     PLISSHVDLS GDPEETVAPA QVALSVTEFG LIGIGDVNPF LAAHPTCPAP GLHSEPLSHC
     NVMTC