MIER2_MOUSE
ID MIER2_MOUSE Reviewed; 541 AA.
AC Q3U3N0; Q5XJI0; Q66JU5; Q69ZP0; Q6NXL7;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Mesoderm induction early response protein 2;
DE Short=Mi-er2;
GN Name=Mier2; Synonyms=Kiaa1193;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Embryonic intestine;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional repressor. {ECO:0000250}.
CC -!- SUBUNIT: Part of a complex containing at least CDYL, MIER1, MIER2,
CC HDAC1 and HDAC2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00512,
CC ECO:0000255|PROSITE-ProRule:PRU00624}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3U3N0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3U3N0-2; Sequence=VSP_030097;
CC Name=3;
CC IsoId=Q3U3N0-3; Sequence=VSP_030096, VSP_030097, VSP_030098;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32406.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK154672; BAE32755.1; -; mRNA.
DR EMBL; AK173128; BAD32406.1; ALT_INIT; mRNA.
DR EMBL; BC067013; AAH67013.2; -; mRNA.
DR EMBL; BC080757; AAH80757.1; -; mRNA.
DR EMBL; BC083321; AAH83321.1; -; mRNA.
DR CCDS; CCDS48619.1; -. [Q3U3N0-2]
DR CCDS; CCDS83723.1; -. [Q3U3N0-1]
DR RefSeq; NP_001303628.1; NM_001316699.1. [Q3U3N0-1]
DR RefSeq; NP_081698.2; NM_027422.3. [Q3U3N0-2]
DR AlphaFoldDB; Q3U3N0; -.
DR BioGRID; 214043; 2.
DR STRING; 10090.ENSMUSP00000059864; -.
DR iPTMnet; Q3U3N0; -.
DR PhosphoSitePlus; Q3U3N0; -.
DR EPD; Q3U3N0; -.
DR MaxQB; Q3U3N0; -.
DR PaxDb; Q3U3N0; -.
DR PeptideAtlas; Q3U3N0; -.
DR PRIDE; Q3U3N0; -.
DR ProteomicsDB; 295565; -. [Q3U3N0-1]
DR ProteomicsDB; 295566; -. [Q3U3N0-2]
DR ProteomicsDB; 295567; -. [Q3U3N0-3]
DR Antibodypedia; 42088; 252 antibodies from 24 providers.
DR DNASU; 70427; -.
DR Ensembl; ENSMUST00000062855; ENSMUSP00000059864; ENSMUSG00000042570. [Q3U3N0-2]
DR Ensembl; ENSMUST00000165028; ENSMUSP00000127387; ENSMUSG00000042570. [Q3U3N0-1]
DR GeneID; 70427; -.
DR KEGG; mmu:70427; -.
DR UCSC; uc007fyw.1; mouse. [Q3U3N0-2]
DR UCSC; uc007fyy.1; mouse. [Q3U3N0-1]
DR CTD; 54531; -.
DR MGI; MGI:1917677; Mier2.
DR VEuPathDB; HostDB:ENSMUSG00000042570; -.
DR eggNOG; KOG4329; Eukaryota.
DR GeneTree; ENSGT01030000234573; -.
DR HOGENOM; CLU_027202_3_1_1; -.
DR InParanoid; Q3U3N0; -.
DR OMA; HRQKAYE; -.
DR OrthoDB; 1062529at2759; -.
DR PhylomeDB; Q3U3N0; -.
DR TreeFam; TF106453; -.
DR BioGRID-ORCS; 70427; 4 hits in 73 CRISPR screens.
DR PRO; PR:Q3U3N0; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q3U3N0; protein.
DR Bgee; ENSMUSG00000042570; Expressed in animal zygote and 139 other tissues.
DR ExpressionAtlas; Q3U3N0; baseline and differential.
DR Genevisible; Q3U3N0; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004407; F:histone deacetylase activity; ISO:MGI.
DR GO; GO:0042826; F:histone deacetylase binding; ISO:MGI.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0016575; P:histone deacetylation; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR000949; ELM2_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR040138; MIER/MTA.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR PANTHER; PTHR10865; PTHR10865; 1.
DR Pfam; PF01448; ELM2; 1.
DR SMART; SM01189; ELM2; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51156; ELM2; 1.
DR PROSITE; PS51293; SANT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..541
FT /note="Mesoderm induction early response protein 2"
FT /id="PRO_0000313679"
FT DOMAIN 194..291
FT /note="ELM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00512"
FT DOMAIN 296..348
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT REGION 100..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..184
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N344"
FT VAR_SEQ 1..36
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15368895"
FT /id="VSP_030096"
FT VAR_SEQ 124..125
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15368895,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_030097"
FT VAR_SEQ 390..397
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15368895"
FT /id="VSP_030098"
SQ SEQUENCE 541 AA; 59268 MW; 8967FE4C67D3B815 CRC64;
MAEASSLERQ SPRVASCLVH SLCPREPSLQ TTAVVSMGSA DHQFNLAELL TQNYNLQEGC
AEAPQCPDKP EEELDKDFID QSSDMPLDEL LALYGYESSD PISEQESEGG DTAPALPDMT
LDKEQIAKDL LSGEEEEETQ SSADDLTPSV TSHEASDLFH NQSGSRFLAG DNGPGSSASS
DTEEDALPAN KCKKEIMVGP QFQADLNILH LNRHCDKIYE NEDQLLWSPS VLPEREVEEF
LYRAVKRRWQ EMAGPQIPEG EVVKDSEQAL YELVKCNFNV EEALRRLRFN VKVIRDGLCA
WSEEECRNFE HGFRVHGKNF HLIQANKVRT RSVGECVEYY YLWKKSERYD YFAQQTRLGR
RKFVSSGTTD TEQDLDGLDP DGHARLHSEA GVPVEPLNVD IEAGGLDQPG VGSDDLPSSE
PGPRPFQQLD EPPAVPSLQQ PTSLAASAEL PPAAAAAPEP GTSPRLPVDL ALPEELPLVS
SPVALSEDTA EPMAPAQVAL SVTEFGLIGI GDVNPFLTGH PACPTSTLHS EPLSQCNVMT
C
