MIER3_HUMAN
ID MIER3_HUMAN Reviewed; 550 AA.
AC Q7Z3K6; B4DRI9; B8ZZQ0; Q5CZI0; Q68CS3; Q6MZS7; Q86YG8; Q8NA13;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Mesoderm induction early response protein 3;
DE Short=Mi-er3;
GN Name=MIER3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 5).
RC TISSUE=Fetal kidney, and Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT GLY-126.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-53, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-53, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-53 AND SER-156, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Transcriptional repressor. {ECO:0000250}.
CC -!- INTERACTION:
CC Q7Z3K6-2; Q96EZ8: MCRS1; NbExp=3; IntAct=EBI-12224671, EBI-348259;
CC Q7Z3K6-2; O75716: STK16; NbExp=3; IntAct=EBI-12224671, EBI-749295;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00512,
CC ECO:0000255|PROSITE-ProRule:PRU00624}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q7Z3K6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z3K6-2; Sequence=VSP_030100;
CC Name=3;
CC IsoId=Q7Z3K6-3; Sequence=VSP_030103;
CC Name=4;
CC IsoId=Q7Z3K6-4; Sequence=VSP_030099;
CC Name=5;
CC IsoId=Q7Z3K6-5; Sequence=VSP_030101, VSP_030102;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH41348.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC04117.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAD97846.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAE45951.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAI56710.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK093273; BAC04117.1; ALT_FRAME; mRNA.
DR EMBL; AK299285; BAG61301.1; -; mRNA.
DR EMBL; BX537798; CAD97846.1; ALT_INIT; mRNA.
DR EMBL; AC008937; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC016644; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX640907; CAE45951.2; ALT_INIT; mRNA.
DR EMBL; BX648294; CAI56710.1; ALT_FRAME; mRNA.
DR EMBL; CR749246; CAH18102.1; -; mRNA.
DR EMBL; CR749805; CAH18665.1; -; mRNA.
DR EMBL; BC041348; AAH41348.2; ALT_INIT; mRNA.
DR CCDS; CCDS3973.2; -. [Q7Z3K6-3]
DR CCDS; CCDS75248.1; -. [Q7Z3K6-1]
DR CCDS; CCDS78011.1; -. [Q7Z3K6-2]
DR RefSeq; NP_001284527.1; NM_001297598.1. [Q7Z3K6-2]
DR RefSeq; NP_001284528.1; NM_001297599.1. [Q7Z3K6-1]
DR RefSeq; NP_689835.3; NM_152622.4. [Q7Z3K6-3]
DR AlphaFoldDB; Q7Z3K6; -.
DR BioGRID; 127939; 24.
DR IntAct; Q7Z3K6; 8.
DR STRING; 9606.ENSP00000370596; -.
DR iPTMnet; Q7Z3K6; -.
DR PhosphoSitePlus; Q7Z3K6; -.
DR BioMuta; MIER3; -.
DR DMDM; 166217020; -.
DR EPD; Q7Z3K6; -.
DR jPOST; Q7Z3K6; -.
DR MassIVE; Q7Z3K6; -.
DR MaxQB; Q7Z3K6; -.
DR PaxDb; Q7Z3K6; -.
DR PeptideAtlas; Q7Z3K6; -.
DR PRIDE; Q7Z3K6; -.
DR ProteomicsDB; 69064; -. [Q7Z3K6-1]
DR ProteomicsDB; 69065; -. [Q7Z3K6-2]
DR ProteomicsDB; 69066; -. [Q7Z3K6-3]
DR ProteomicsDB; 69067; -. [Q7Z3K6-4]
DR ProteomicsDB; 69068; -. [Q7Z3K6-5]
DR Antibodypedia; 23538; 120 antibodies from 22 providers.
DR DNASU; 166968; -.
DR Ensembl; ENST00000381199.8; ENSP00000370596.3; ENSG00000155545.20. [Q7Z3K6-1]
DR Ensembl; ENST00000381213.7; ENSP00000370611.3; ENSG00000155545.20. [Q7Z3K6-3]
DR Ensembl; ENST00000381226.7; ENSP00000370624.3; ENSG00000155545.20. [Q7Z3K6-2]
DR Ensembl; ENST00000409421.5; ENSP00000386584.1; ENSG00000155545.20. [Q7Z3K6-4]
DR Ensembl; ENST00000452157.5; ENSP00000408076.1; ENSG00000155545.20. [Q7Z3K6-5]
DR GeneID; 166968; -.
DR KEGG; hsa:166968; -.
DR MANE-Select; ENST00000381199.8; ENSP00000370596.3; NM_001297599.2; NP_001284528.1.
DR UCSC; uc003jqz.2; human. [Q7Z3K6-1]
DR CTD; 166968; -.
DR DisGeNET; 166968; -.
DR GeneCards; MIER3; -.
DR HGNC; HGNC:26678; MIER3.
DR HPA; ENSG00000155545; Low tissue specificity.
DR neXtProt; NX_Q7Z3K6; -.
DR OpenTargets; ENSG00000155545; -.
DR PharmGKB; PA145008088; -.
DR VEuPathDB; HostDB:ENSG00000155545; -.
DR eggNOG; KOG4329; Eukaryota.
DR GeneTree; ENSGT01030000234573; -.
DR HOGENOM; CLU_027202_2_0_1; -.
DR InParanoid; Q7Z3K6; -.
DR OMA; NQMNMCS; -.
DR OrthoDB; 1062529at2759; -.
DR PhylomeDB; Q7Z3K6; -.
DR TreeFam; TF106453; -.
DR PathwayCommons; Q7Z3K6; -.
DR SignaLink; Q7Z3K6; -.
DR BioGRID-ORCS; 166968; 13 hits in 1105 CRISPR screens.
DR GenomeRNAi; 166968; -.
DR Pharos; Q7Z3K6; Tdark.
DR PRO; PR:Q7Z3K6; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q7Z3K6; protein.
DR Bgee; ENSG00000155545; Expressed in ileal mucosa and 183 other tissues.
DR ExpressionAtlas; Q7Z3K6; baseline and differential.
DR Genevisible; Q7Z3K6; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IDA:UniProtKB.
DR GO; GO:0042826; F:histone deacetylase binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR000949; ELM2_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR040138; MIER/MTA.
DR InterPro; IPR045787; MIER1/3_C.
DR InterPro; IPR031156; MIER3.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR PANTHER; PTHR10865; PTHR10865; 1.
DR PANTHER; PTHR10865:SF22; PTHR10865:SF22; 1.
DR Pfam; PF01448; ELM2; 1.
DR Pfam; PF19426; MIER1_beta_C; 1.
DR SMART; SM01189; ELM2; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51156; ELM2; 1.
DR PROSITE; PS51293; SANT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..550
FT /note="Mesoderm induction early response protein 3"
FT /id="PRO_0000313680"
FT DOMAIN 174..272
FT /note="ELM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00512"
FT DOMAIN 277..329
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 52
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163"
FT MOD_RES 53
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UHF3"
FT MOD_RES 156
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 163
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q3UHF3"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UHF3"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UHF3"
FT VAR_SEQ 1..63
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_030099"
FT VAR_SEQ 11
FT /note="P -> PVCFIP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_030100"
FT VAR_SEQ 106..119
FT /note="EEIAKDLLSGDDEE -> ISGRERNPHLGTSS (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_030101"
FT VAR_SEQ 120..550
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_030102"
FT VAR_SEQ 277
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:17974005"
FT /id="VSP_030103"
FT VARIANT 126
FT /note="D -> G (in dbSNP:rs17854108)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_037699"
FT VARIANT 414
FT /note="N -> K (in dbSNP:rs13177917)"
FT /id="VAR_037700"
FT VARIANT 432
FT /note="N -> D (in dbSNP:rs13181823)"
FT /id="VAR_037701"
FT VARIANT 530
FT /note="N -> S (in dbSNP:rs36017815)"
FT /id="VAR_037702"
FT CONFLICT 5
FT /note="S -> L (in Ref. 2; CAI56710)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 550 AA; 61437 MW; F2A75EC59477C54F CRC64;
MAEASFGSSS PVGSLSSEDH DFDPTAEMLV HDYDDERTLE EEEMMDEGKN FSSEIEDLEK
EGTMPLEDLL AFYGYEPTIP AVANSSANSS PSELADELPD MTLDKEEIAK DLLSGDDEET
QSSADDLTPS VTSHETSDFF PRPLRSNTAC DGDKESEVED VETDSGNSPE DLRKEIMIGL
QYQAEIPPYL GEYDGNEKVY ENEDQLLWCP DVVLESKVKE YLVETSLRTG SEKIMDRISA
GTHTRDNEQA LYELLKCNHN IKEAIERYCC NGKASQEGMT AWTEEECRSF EHALMLFGKD
FHLIQKNKVR TRTVAECVAF YYMWKKSERY DYFAQQTRFG KKRYNHHPGV TDYMDRLVDE
TEALGGTVNA SALTSNRPEP IPDQQLNILN SFTASDLTAL TNSVATVCDP TDVNCLDDSF
PPLGNTPRGQ VNHVPVVTEE LLTLPSNGES DCFNLFETGF YHSELNPMNM CSEESERPAK
RLKMGIAVPE SFMNEVSVNN LGVDFENHTH HITSAKMAVS VADFGSLSAN ETNGFISAHA
LHQHAALHSE
