MIER3_MOUSE
ID MIER3_MOUSE Reviewed; 551 AA.
AC Q3UHF3; Q3SYJ7; Q3UGR9; Q8BND7; Q8BSS0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Mesoderm induction early response protein 3;
DE Short=Mi-er3;
GN Name=Mier3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, Embryo, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-551 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-156; THR-163 AND
RP SER-165, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcriptional repressor. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00512,
CC ECO:0000255|PROSITE-ProRule:PRU00624}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UHF3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UHF3-2; Sequence=VSP_030104, VSP_030105;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI03781.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK030747; BAC27115.1; -; mRNA.
DR EMBL; AK083929; BAC39070.1; -; mRNA.
DR EMBL; AK147427; BAE27904.1; -; mRNA.
DR EMBL; AK147788; BAE28138.1; -; mRNA.
DR EMBL; BC103780; AAI03781.1; ALT_INIT; mRNA.
DR CCDS; CCDS26770.2; -. [Q3UHF3-1]
DR RefSeq; NP_766181.2; NM_172593.3. [Q3UHF3-1]
DR RefSeq; XP_006517680.1; XM_006517617.1. [Q3UHF3-2]
DR AlphaFoldDB; Q3UHF3; -.
DR STRING; 10090.ENSMUSP00000104895; -.
DR iPTMnet; Q3UHF3; -.
DR PhosphoSitePlus; Q3UHF3; -.
DR EPD; Q3UHF3; -.
DR MaxQB; Q3UHF3; -.
DR PaxDb; Q3UHF3; -.
DR PRIDE; Q3UHF3; -.
DR ProteomicsDB; 295667; -. [Q3UHF3-1]
DR ProteomicsDB; 295668; -. [Q3UHF3-2]
DR Antibodypedia; 23538; 120 antibodies from 22 providers.
DR DNASU; 218613; -.
DR Ensembl; ENSMUST00000047412; ENSMUSP00000036809; ENSMUSG00000032727. [Q3UHF3-2]
DR Ensembl; ENSMUST00000109272; ENSMUSP00000104895; ENSMUSG00000032727. [Q3UHF3-1]
DR GeneID; 218613; -.
DR KEGG; mmu:218613; -.
DR UCSC; uc007rvy.1; mouse. [Q3UHF3-1]
DR UCSC; uc007rvz.1; mouse. [Q3UHF3-2]
DR CTD; 166968; -.
DR MGI; MGI:2442317; Mier3.
DR VEuPathDB; HostDB:ENSMUSG00000032727; -.
DR eggNOG; KOG4329; Eukaryota.
DR GeneTree; ENSGT01030000234573; -.
DR HOGENOM; CLU_027202_2_0_1; -.
DR InParanoid; Q3UHF3; -.
DR OMA; NQMNMCS; -.
DR OrthoDB; 1062529at2759; -.
DR PhylomeDB; Q3UHF3; -.
DR TreeFam; TF106453; -.
DR BioGRID-ORCS; 218613; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Mier3; mouse.
DR PRO; PR:Q3UHF3; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q3UHF3; protein.
DR Bgee; ENSMUSG00000032727; Expressed in spermatocyte and 238 other tissues.
DR ExpressionAtlas; Q3UHF3; baseline and differential.
DR Genevisible; Q3UHF3; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0042826; F:histone deacetylase binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR000949; ELM2_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR040138; MIER/MTA.
DR InterPro; IPR045787; MIER1/3_C.
DR InterPro; IPR031156; MIER3.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR PANTHER; PTHR10865; PTHR10865; 1.
DR PANTHER; PTHR10865:SF22; PTHR10865:SF22; 1.
DR Pfam; PF01448; ELM2; 1.
DR Pfam; PF19426; MIER1_beta_C; 1.
DR SMART; SM01189; ELM2; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51156; ELM2; 1.
DR PROSITE; PS51293; SANT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..551
FT /note="Mesoderm induction early response protein 3"
FT /id="PRO_0000313682"
FT DOMAIN 174..273
FT /note="ELM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00512"
FT DOMAIN 278..330
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 52
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z3K6"
FT MOD_RES 53
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z3K6"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 156
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 163
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT VAR_SEQ 1..27
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030104"
FT VAR_SEQ 278
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030105"
FT CONFLICT 52
FT /note="S -> G (in Ref. 2; AAI03781)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="E -> K (in Ref. 1; BAE27904)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="V -> L (in Ref. 1; BAC39070)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 551 AA; 61521 MW; E4354B466C6903FA CRC64;
MAEASFGSSS PVGSLSSEDH DFDPTAEMLV HDYDDERTLE EEELMDDGKN FSSEIEDLEK
EGNMPLEDLL AFYGYESTIP AVANSSANSS PSELADELPD MTLDKEEIAK DLLSGDDEET
QSSADDLTPS VTSHETSEFF PRPLRSNTTC DGDKESEIED VETDSGNSPE DLRREIMIGL
EYQAEIPPYL GEYNGDDEKA YENEDQLLWH PGVLLESKVK EYLVETSLRT GNEKVLDRIS
SGTHTRDNEQ ALYELLKCNH NIKEAIERYC CNGKASQEGM TAWTEEECRS FEHALMLHGK
DFHLIQKDKV RSRTVAECVA FYYMWKKSER YDYFAQQTKF GKKRYNHHPG VTDYMDRLVD
ETESLGGTVS SSALTCNRPE PVPDQPLNIL SSFTASDLTA LTNSVATVCN PTAVNCLDDS
FPPLANTPRG HVNHVPVVTE ELLTLPSNGE SDCFNLFETG FYHSELNPMC SEESERPAKR
LKMGIAVPES FMNEVSVNNL GVDFENHTHH ITSAKMAVSV ADFGSLSANE TNGFINAHSL
HQHQHAALHS E
