MIFH_BRUMA
ID MIFH_BRUMA Reviewed; 115 AA.
AC P91850; A8PJU3; O02555; Q8IHI7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 4.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Macrophage migration inhibitory factor homolog;
DE Short=Bm-MIF-1;
DE Short=BmMIF;
DE Short=MIF;
DE EC=5.3.2.1;
DE AltName: Full=L-dopachrome isomerase;
DE AltName: Full=L-dopachrome tautomerase;
DE EC=5.3.3.12;
DE AltName: Full=Phenylpyruvate tautomerase;
GN ORFNames=Bm1_28435, BMBAC01P19.1;
OS Brugia malayi (Filarial nematode worm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia.
OX NCBI_TaxID=6279;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=TRW;
RX PubMed=9826378; DOI=10.1128/iai.66.12.5955-5963.1998;
RA Pastrana D.V., Raghavan N., Fitzgerald P., Eisinger S.W., Metz C.,
RA Bucala R., Schleimer R.P., Bickel C., Scott A.L.;
RT "Filarial nematode parasites secrete a homologue of the human cytokine
RT macrophage migration inhibitory factor.";
RL Infect. Immun. 66:5955-5963(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Gulliano D.B., Hall N., Jones S.J.M., Clark L.N., Corton C.H.,
RA Barrell B.G., Blaxter M.L.;
RT "Conservation of long-range and micro-synteny between the genomes of two
RT distantly related nematodes.";
RL Genome Biol. 3:RESEARCH0057.1-RESEARCH0057.14(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17885136; DOI=10.1126/science.1145406;
RA Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E.,
RA Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T.,
RA Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L.,
RA Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M.,
RA Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J.,
RA Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F.,
RA Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W.,
RA Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M.,
RA McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J.,
RA Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E.,
RA Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G.,
RA Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B.,
RA Blaxter M.L., Scott A.L.;
RT "Draft genome of the filarial nematode parasite Brugia malayi.";
RL Science 317:1756-1760(2007).
CC -!- FUNCTION: Tautomerization of the methyl ester of L-dopachrome (By
CC similarity). Inhibits migration of human peripheral blood mononuclear
CC cells. {ECO:0000250, ECO:0000269|PubMed:9826378}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate;
CC Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509;
CC EC=5.3.3.12;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-phenylpyruvate = enol-phenylpyruvate; Xref=Rhea:RHEA:17097,
CC ChEBI:CHEBI:16815, ChEBI:CHEBI:18005; EC=5.3.2.1;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9826378}.
CC -!- SIMILARITY: Belongs to the MIF family. {ECO:0000305}.
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DR EMBL; AF002699; AAB60943.1; -; Genomic_DNA.
DR EMBL; U88035; AAC82502.1; -; mRNA.
DR EMBL; AL606837; CAC70155.1; -; Genomic_DNA.
DR EMBL; DS239369; EDP34019.1; -; Genomic_DNA.
DR RefSeq; XP_001897136.1; XM_001897101.1.
DR AlphaFoldDB; P91850; -.
DR SMR; P91850; -.
DR STRING; 6279.P91850; -.
DR EnsemblMetazoa; Bm6870.1; Bm6870.1; WBGene00227131.
DR GeneID; 6100584; -.
DR KEGG; bmy:BM_BM6870; -.
DR WBParaSite; Bm6870.1; Bm6870.1; WBGene00227131.
DR CTD; 6100584; -.
DR WormBase; Bm6870; BM07480; WBGene00227131; Bma-mif-1.
DR InParanoid; P91850; -.
DR OMA; YINFFDM; -.
DR OrthoDB; 1451925at2759; -.
DR Proteomes; UP000006672; Unassembled WGS sequence.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0004167; F:dopachrome isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0050178; F:phenylpyruvate tautomerase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.429.10; -; 1.
DR InterPro; IPR001398; Macrophage_inhib_fac.
DR InterPro; IPR019829; Macrophage_inhib_fac_CS.
DR InterPro; IPR014347; Tautomerase/MIF_sf.
DR PANTHER; PTHR11954; PTHR11954; 1.
DR Pfam; PF01187; MIF; 1.
DR SUPFAM; SSF55331; SSF55331; 1.
DR PROSITE; PS01158; MIF; 1.
PE 3: Inferred from homology;
KW Cytokine; Isomerase; Reference proteome; Secreted.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..115
FT /note="Macrophage migration inhibitory factor homolog"
FT /id="PRO_0000158073"
FT ACT_SITE 2
FT /note="Proton acceptor; via imino nitrogen"
FT /evidence="ECO:0000250"
FT BINDING 33
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 65
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 114
FT /note="F -> L (in Ref. 1; AAB60943/AAC82502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 115 AA; 12320 MW; F4D2FE1B56630E73 CRC64;
MPYFTIDTNI PQNSISSAFL KKASNVVAKA LGKPESYVSI HVNGGQAMVF GGSEDPCAVC
VLKSIGCVGP KVNNSHAEKL YKLLADELKI PKNRCYIEFV DIEASSMAFN GSTFG
