ID   MIFH_TRITR              Reviewed;         114 AA.
AC   P81748; Q9U920;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Macrophage migration inhibitory factor homolog;
DE            Short=MIF;
DE            EC=5.3.2.1;
DE   AltName: Full=L-dopachrome isomerase;
DE   AltName: Full=L-dopachrome tautomerase;
DE            EC=5.3.3.12;
DE   AltName: Full=Phenylpyruvate tautomerase;
OS   Trichuris trichiura (Whipworm) (Trichocephalus trichiurus).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC   Trichinellida; Trichuridae; Trichuris.
OX   NCBI_TaxID=36087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-34; 48-69 AND 80-109, AND
RP   CATALYTIC ACTIVITY.
RX   PubMed=11439086; DOI=10.1042/0264-6021:3570373;
RA   Tan T.H.P., Edgerton S.A.V., Kumari R., McAlister M.S.B., Roe S.M.,
RA   Nagl S., Pearl L.H., Selkirk M.E., Bianco A.E., Totty N.F., Engwerda C.,
RA   Gray C.A., Meyer D.J., Rowe S.M.;
RT   "Macrophage migration inhibitory factor of the parasitic nematode
RT   Trichinella spiralis.";
RL   Biochem. J. 357:373-383(2001).
CC   -!- FUNCTION: Tautomerization of the methyl ester of L-dopachrome. Inhibits
CC       migration of human peripheral blood mononuclear cells (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate;
CC         Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509;
CC         EC=5.3.3.12; Evidence={ECO:0000269|PubMed:11439086};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-phenylpyruvate = enol-phenylpyruvate; Xref=Rhea:RHEA:17097,
CC         ChEBI:CHEBI:16815, ChEBI:CHEBI:18005; EC=5.3.2.1;
CC         Evidence={ECO:0000269|PubMed:11439086};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MIF family. {ECO:0000305}.
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DR   EMBL; AJ237770; CAB46355.1; -; mRNA.
DR   AlphaFoldDB; P81748; -.
DR   SMR; P81748; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0004167; F:dopachrome isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050178; F:phenylpyruvate tautomerase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.30.429.10; -; 1.
DR   InterPro; IPR001398; Macrophage_inhib_fac.
DR   InterPro; IPR014347; Tautomerase/MIF_sf.
DR   PANTHER; PTHR11954; PTHR11954; 1.
DR   Pfam; PF01187; MIF; 1.
DR   SUPFAM; SSF55331; SSF55331; 1.
PE   1: Evidence at protein level;
KW   Cytokine; Direct protein sequencing; Isomerase; Secreted.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:11439086"
FT   CHAIN           2..114
FT                   /note="Macrophage migration inhibitory factor homolog"
FT                   /id="PRO_0000158076"
FT   ACT_SITE        2
FT                   /note="Proton acceptor; via imino nitrogen"
FT                   /evidence="ECO:0000250"
FT   BINDING         33
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   114 AA;  12043 MW;  43B3BD9A9C77215B CRC64;
     MPIFTFSTNV PSENISVDFL KSTSKLIAGM LGKPESYVAV HINGGQKITF GGTDAPAGFG
     QLLSLGGVGG EKNRSHSAKL FKHLTDGLGI PGNRMYINFV DMRGSDVGYN GSTF