ID   MIFH_WUCBA              Reviewed;         115 AA.
AC   O44786;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Macrophage migration inhibitory factor homolog;
DE            Short=MIF;
DE            EC=5.3.2.1;
DE   AltName: Full=L-dopachrome isomerase;
DE   AltName: Full=L-dopachrome tautomerase;
DE            EC=5.3.3.12;
DE   AltName: Full=Phenylpyruvate tautomerase;
GN   Name=MIF;
OS   Wuchereria bancrofti.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Wuchereria.
OX   NCBI_TaxID=6293;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Brazil;
RA   Scott A.L., Josh S., Pastrana D., Eisinger S.W., Marson A., Ragahavan N.;
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Tautomerization of the methyl ester of L-dopachrome. Inhibits
CC       migration of human peripheral blood mononuclear cells (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate;
CC         Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509;
CC         EC=5.3.3.12;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-phenylpyruvate = enol-phenylpyruvate; Xref=Rhea:RHEA:17097,
CC         ChEBI:CHEBI:16815, ChEBI:CHEBI:18005; EC=5.3.2.1;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MIF family. {ECO:0000305}.
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DR   EMBL; AF040629; AAC82615.1; -; mRNA.
DR   AlphaFoldDB; O44786; -.
DR   SMR; O44786; -.
DR   STRING; 6293.O44786; -.
DR   Proteomes; UP000093561; Unassembled WGS sequence.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0004167; F:dopachrome isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050178; F:phenylpyruvate tautomerase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.30.429.10; -; 1.
DR   InterPro; IPR001398; Macrophage_inhib_fac.
DR   InterPro; IPR019829; Macrophage_inhib_fac_CS.
DR   InterPro; IPR014347; Tautomerase/MIF_sf.
DR   PANTHER; PTHR11954; PTHR11954; 1.
DR   Pfam; PF01187; MIF; 1.
DR   SUPFAM; SSF55331; SSF55331; 1.
DR   PROSITE; PS01158; MIF; 1.
PE   3: Inferred from homology;
KW   Cytokine; Isomerase; Secreted.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..115
FT                   /note="Macrophage migration inhibitory factor homolog"
FT                   /id="PRO_0000158077"
FT   ACT_SITE        2
FT                   /note="Proton acceptor; via imino nitrogen"
FT                   /evidence="ECO:0000250"
FT   BINDING         33
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         65
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   115 AA;  12364 MW;  FEF29CBBFCD2A8E6 CRC64;
     MPYFTIDTNK PQDSISSAFL KKAPNVVPKA LGKPESYVSI HVNGGQPMVF GGSEDPCPVC
     VLKSIGCVGP KVNNSHAEKL YKLLADELKI PKNRCYIESV DIEASSMAFN GSTFG