MIG2_YEAST
ID MIG2_YEAST Reviewed; 382 AA.
AC P53035; D6VTU6; O11853; O11854;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Regulatory protein MIG2;
GN Name=MIG2; Synonyms=MLZ1; OrderedLocusNames=YGL209W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8756637; DOI=10.1128/mcb.16.9.4790;
RA Lutfiyya L.L., Johnston M.;
RT "Two zinc-finger-containing repressors are responsible for glucose
RT repression of SUC2 expression.";
RL Mol. Cell. Biol. 16:4790-4797(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8813766;
RX DOI=10.1002/(sici)1097-0061(19960630)12:8<799::aid-yea965>3.0.co;2-u;
RA Kail M., Juettner E., Vaux D.;
RT "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces
RT cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and
RT containing three novel transcribed open reading frames.";
RL Yeast 12:799-807(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9153757;
RX DOI=10.1002/(sici)1097-0061(199704)13:5<475::aid-yea101>3.0.co;2-0;
RA Feuermann M., Simeonava L., Souciet J.-L., Potier S.;
RT "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII
RT reveals 11 open reading frames: two correspond to new genes.";
RL Yeast 13:475-477(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in glucose repression of the SUC2 gene.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the creA/MIG C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; U54564; AAB37570.1; -; Genomic_DNA.
DR EMBL; U33754; AAC49496.1; -; Genomic_DNA.
DR EMBL; Z72731; CAA96923.2; -; Genomic_DNA.
DR EMBL; Z72731; CAA96924.1; -; Genomic_DNA.
DR EMBL; Z72730; CAA96921.1; -; Genomic_DNA.
DR EMBL; Z72732; CAA96925.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07907.1; -; Genomic_DNA.
DR PIR; S71669; S71669.
DR RefSeq; NP_011306.1; NM_001181074.1.
DR AlphaFoldDB; P53035; -.
DR SMR; P53035; -.
DR BioGRID; 33047; 109.
DR IntAct; P53035; 7.
DR STRING; 4932.YGL209W; -.
DR MaxQB; P53035; -.
DR PaxDb; P53035; -.
DR PRIDE; P53035; -.
DR EnsemblFungi; YGL209W_mRNA; YGL209W; YGL209W.
DR GeneID; 852663; -.
DR KEGG; sce:YGL209W; -.
DR SGD; S000003177; MIG2.
DR VEuPathDB; FungiDB:YGL209W; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000169836; -.
DR HOGENOM; CLU_039422_0_0_1; -.
DR InParanoid; P53035; -.
DR OMA; HQPIPQV; -.
DR BioCyc; YEAST:G3O-30686-MON; -.
DR PRO; PR:P53035; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53035; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0000433; P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose; IDA:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:1900436; P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation; IGI:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..382
FT /note="Regulatory protein MIG2"
FT /id="PRO_0000046882"
FT ZN_FING 17..39
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 45..69
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 252..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 382 AA; 42048 MW; BE25CBC21FB962C6 CRC64;
MPKKQTNFPV DNENRPFRCD TCHRGFHRLE HKKRHLRTHT GEKPHHCAFP GCGKSFSRSD
ELKRHMRTHT GQSQRRLKKA SVQKQEFLTV SGIPTIASGV MIHQPIPQVL PANMAINVQA
VNGGNIIHAP NAVHPMVIPI MAQPAPIHAS AASFQPATSP MPISTYTPVP SQSFTSFQSS
IGSIQSNSDV SSIFSNMNVR VNTPRSVPNS PNDGYLHQQH IPQQYQHQTA SPSVAKQQKT
FAHSLASALS TLQKRTPVSA PSTTIESPSS PSDSSHTSAS SSAISLPFSN APSQLAVAKE
LESVYLDSNR YTTKTRRERA KFEIPEEQEE DTNNSSSGSN EEEHESLDHE SSKSRKKLSG
VKLPPVRNLL KQIDVFNGPK RV
