MIGA1_MOUSE
ID MIGA1_MOUSE Reviewed; 600 AA.
AC Q4QQM5; Q8BR91; Q8C3S6; Q8C8P4;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Mitoguardin 1;
DE AltName: Full=Protein FAM73A {ECO:0000305};
GN Name=Miga1 {ECO:0000250|UniProtKB:Q8NAN2};
GN Synonyms=Fam73a {ECO:0000312|MGI:MGI:1924567};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, Lung, and Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-257 AND SER-261, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26711011; DOI=10.1016/j.molcel.2015.11.017;
RA Zhang Y., Liu X., Bai J., Tian X., Zhao X., Liu W., Duan X., Shang W.,
RA Fan H.Y., Tong C.;
RT "Mitoguardin regulates mitochondrial fusion through MitoPLD and is required
RT for neuronal homeostasis.";
RL Mol. Cell 61:111-124(2016).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=26716412; DOI=10.18632/oncotarget.6713;
RA Liu X.M., Zhang Y.P., Ji S.Y., Li B.T., Tian X., Li D., Tong C., Fan H.Y.;
RT "Mitoguardin-1 and -2 promote maturation and the developmental potential of
RT mouse oocytes by maintaining mitochondrial dynamics and functions.";
RL Oncotarget 7:1155-1167(2016).
CC -!- FUNCTION: Regulator of mitochondrial fusion (PubMed:26711011). Acts by
CC forming homo- and heterodimers at the mitochondrial outer membrane and
CC facilitating the formation of PLD6/MitoPLD dimers. May act by
CC regulating phospholipid metabolism via PLD6/MitoPLD (By similarity).
CC {ECO:0000250|UniProtKB:Q8NAN2, ECO:0000269|PubMed:26711011}.
CC -!- SUBUNIT: Homodimer and heterodimer; forms heterodimers with MIGA2.
CC Interacts with PLD6/MitoPLD. {ECO:0000250|UniProtKB:Q8NAN2}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000250|UniProtKB:Q8NAN2}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q4QQM5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4QQM5-2; Sequence=VSP_024881, VSP_024882;
CC Name=3;
CC IsoId=Q4QQM5-3; Sequence=VSP_024880;
CC -!- DISRUPTION PHENOTYPE: Mitochondrial fragmentation: mitochondria become
CC round and show loss of cristae (PubMed:26711011). Female mice show
CC decreased quality of oocytes (PubMed:26716412). Mice lacking both Miga1
CC and Miga2 show strongly reduced quality of oocytes and are subfertile
CC (PubMed:26716412). {ECO:0000269|PubMed:26711011,
CC ECO:0000269|PubMed:26716412}.
CC -!- SIMILARITY: Belongs to the mitoguardin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC32059.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK044739; BAC32059.1; ALT_FRAME; mRNA.
DR EMBL; AK045347; BAC32322.1; -; mRNA.
DR EMBL; AK085017; BAC39340.1; -; mRNA.
DR EMBL; BC098205; AAH98205.1; -; mRNA.
DR CCDS; CCDS17916.1; -. [Q4QQM5-1]
DR CCDS; CCDS51096.1; -. [Q4QQM5-3]
DR RefSeq; NP_001155847.1; NM_001162375.1. [Q4QQM5-3]
DR RefSeq; NP_777357.2; NM_174868.4. [Q4QQM5-1]
DR AlphaFoldDB; Q4QQM5; -.
DR STRING; 10090.ENSMUSP00000072836; -.
DR iPTMnet; Q4QQM5; -.
DR PhosphoSitePlus; Q4QQM5; -.
DR MaxQB; Q4QQM5; -.
DR PaxDb; Q4QQM5; -.
DR PeptideAtlas; Q4QQM5; -.
DR PRIDE; Q4QQM5; -.
DR ProteomicsDB; 290242; -. [Q4QQM5-1]
DR ProteomicsDB; 290243; -. [Q4QQM5-2]
DR ProteomicsDB; 290244; -. [Q4QQM5-3]
DR Antibodypedia; 2672; 30 antibodies from 13 providers.
DR DNASU; 215708; -.
DR Ensembl; ENSMUST00000068243; ENSMUSP00000068261; ENSMUSG00000054942. [Q4QQM5-3]
DR Ensembl; ENSMUST00000073089; ENSMUSP00000072836; ENSMUSG00000054942. [Q4QQM5-1]
DR Ensembl; ENSMUST00000199334; ENSMUSP00000143238; ENSMUSG00000054942. [Q4QQM5-2]
DR GeneID; 215708; -.
DR KEGG; mmu:215708; -.
DR UCSC; uc008rte.2; mouse. [Q4QQM5-1]
DR UCSC; uc008rtf.2; mouse. [Q4QQM5-2]
DR UCSC; uc012czl.1; mouse. [Q4QQM5-3]
DR CTD; 374986; -.
DR MGI; MGI:1924567; Miga1.
DR VEuPathDB; HostDB:ENSMUSG00000054942; -.
DR eggNOG; KOG3831; Eukaryota.
DR GeneTree; ENSGT00390000008565; -.
DR HOGENOM; CLU_031519_1_0_1; -.
DR InParanoid; Q4QQM5; -.
DR OMA; WENTEAE; -.
DR PhylomeDB; Q4QQM5; -.
DR TreeFam; TF313896; -.
DR Reactome; R-MMU-1483166; Synthesis of PA.
DR BioGRID-ORCS; 215708; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q4QQM5; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q4QQM5; protein.
DR Bgee; ENSMUSG00000054942; Expressed in dorsomedial nucleus of hypothalamus and 219 other tissues.
DR ExpressionAtlas; Q4QQM5; baseline and differential.
DR Genevisible; Q4QQM5; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0008053; P:mitochondrial fusion; IMP:UniProtKB.
DR InterPro; IPR019392; Miga.
DR PANTHER; PTHR21508; PTHR21508; 1.
DR Pfam; PF10265; Miga; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..600
FT /note="Mitoguardin 1"
FT /id="PRO_0000285647"
FT TRANSMEM 15..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 257
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 261
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 302..365
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024880"
FT VAR_SEQ 490..506
FT /note="ISDGFFAHFYAICEHVS -> VKLTMPQTPPQFILCGF (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024881"
FT VAR_SEQ 507..600
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024882"
FT CONFLICT 321
FT /note="F -> L (in Ref. 1; BAC39340)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 600 AA; 67536 MW; F29D8E90E4835B71 CRC64;
MSDETVSRSQ FSLKTYAVRV FALPVSWYYS LSQIKFSPVA KKLFMVTAVS AVSVIFLAHH
FKRRRGKQKG KVLPWEPEHL LLEHTRRAAS EKGSSCSSSR QNLTLSLSST KEKGSQCCNY
PNGGLLSRYS GSAQSLGSVQ SVNSCHSCAC GNSNSWDKAD DDDIRLVNIP VTTPENLYLM
GMELFEEALR RWEQALTFRS RQAEDEACSS VKLGAGDAIA EESVDDIISS EFIHKLEALL
QRAYRLQEEF EATLGGSDPN SIANDTDKDT DMSLRETMDE LGLPDAMNMD SADLFASATE
LAEQREAQQT FSLESFCPCP FYEEAMHLVE EGKIYSRVLR TEMLECLGDS DFLAKLHCIR
QAFQLILAEA DNRSFLAESG RKILSALIVK ARKNPKKFQD VFDEMINFLE QTDHWDSTEL
ELAARGVKNL NFYDVVLDFI LMDSFEDLEN PPTSIQSVVN NRWLNSSFKE SAVASSCWSV
LKQKRQQMKI SDGFFAHFYA ICEHVSPVLA WGFLGPRNSL YDLCCFFKNQ VLFFLKDIFD
FEKVRYSSID TLAEDLTHLL IRRTELLVTC LGADALRHAT TCTSGHSHAV PTALLEAKVQ
