MIGA_DROME
ID MIGA_DROME Reviewed; 525 AA.
AC Q9W3F7; Q95S64;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Mitoguardin {ECO:0000303|PubMed:26711011};
GN Name=Miga {ECO:0000303|PubMed:26711011, ECO:0000312|FlyBase:FBgn0030037};
GN ORFNames=CG12125 {ECO:0000312|FlyBase:FBgn0030037};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ZUC, AND MUTAGENESIS OF
RP GLY-34.
RX PubMed=26711011; DOI=10.1016/j.molcel.2015.11.017;
RA Zhang Y., Liu X., Bai J., Tian X., Zhao X., Liu W., Duan X., Shang W.,
RA Fan H.Y., Tong C.;
RT "Mitoguardin regulates mitochondrial fusion through MitoPLD and is required
RT for neuronal homeostasis.";
RL Mol. Cell 61:111-124(2016).
CC -!- FUNCTION: Regulator of mitochondrial fusion required to maintain
CC neuronal homeostasis. {ECO:0000269|PubMed:26711011}.
CC -!- SUBUNIT: Interacts with zuc. {ECO:0000269|PubMed:26711011}.
CC -!- INTERACTION:
CC Q9W3F7; Q9VKD7: zuc; NbExp=2; IntAct=EBI-184365, EBI-9943000;
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000305|PubMed:26711011}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the mitoguardin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28486.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF46370.2; -; Genomic_DNA.
DR EMBL; AE014298; ACZ95237.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59488.1; -; Genomic_DNA.
DR EMBL; AY060938; AAL28486.1; ALT_FRAME; mRNA.
DR EMBL; AY113409; AAM29414.1; -; mRNA.
DR EMBL; BT056253; ACL68700.1; -; mRNA.
DR RefSeq; NP_001162702.1; NM_001169231.2.
DR RefSeq; NP_001285017.1; NM_001298088.1.
DR RefSeq; NP_572476.2; NM_132248.3.
DR AlphaFoldDB; Q9W3F7; -.
DR IntAct; Q9W3F7; 1.
DR STRING; 7227.FBpp0071189; -.
DR TCDB; 8.A.167.1.1; the mitoguardin (miga) family.
DR PaxDb; Q9W3F7; -.
DR PRIDE; Q9W3F7; -.
DR EnsemblMetazoa; FBtr0071246; FBpp0071189; FBgn0030037.
DR EnsemblMetazoa; FBtr0300383; FBpp0289612; FBgn0030037.
DR EnsemblMetazoa; FBtr0345986; FBpp0311873; FBgn0030037.
DR GeneID; 31775; -.
DR KEGG; dme:Dmel_CG12125; -.
DR UCSC; CG12125-RA; d. melanogaster.
DR UCSC; CG12125-RB; d. melanogaster.
DR CTD; 31775; -.
DR FlyBase; FBgn0030037; Miga.
DR VEuPathDB; VectorBase:FBgn0030037; -.
DR eggNOG; KOG3831; Eukaryota.
DR GeneTree; ENSGT00390000008565; -.
DR HOGENOM; CLU_031519_0_1_1; -.
DR InParanoid; Q9W3F7; -.
DR OMA; YDICLDF; -.
DR OrthoDB; 341855at2759; -.
DR PhylomeDB; Q9W3F7; -.
DR Reactome; R-DME-1483166; Synthesis of PA.
DR BioGRID-ORCS; 31775; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Miga; fly.
DR GenomeRNAi; 31775; -.
DR PRO; PR:Q9W3F7; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030037; Expressed in egg chamber and 26 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0008053; P:mitochondrial fusion; IMP:UniProtKB.
DR GO; GO:0070584; P:mitochondrion morphogenesis; IMP:FlyBase.
DR GO; GO:0045494; P:photoreceptor cell maintenance; IMP:FlyBase.
DR GO; GO:0010635; P:regulation of mitochondrial fusion; IMP:FlyBase.
DR InterPro; IPR019392; Miga.
DR PANTHER; PTHR21508; PTHR21508; 1.
DR Pfam; PF10265; Miga; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..525
FT /note="Mitoguardin"
FT /id="PRO_0000435630"
FT TRANSMEM 26..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 47..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..65
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 34
FT /note="G->D: In mitoguardian; progressive neurodegeneration
FT in the eye caused by mitochondrial defects. Abolishes
FT localization to the mitochondrion."
FT /evidence="ECO:0000269|PubMed:26711011"
SQ SEQUENCE 525 AA; 59024 MW; 198D25BBDCD21B70 CRC64;
MQRVMPASWS SGGSLLPFRV STTTKVVLFS LTAGVALMSV LSRFLRRRKP PRPPRRARKY
TGRRNRNSMR SPNDLISIAG SKASARSGSP VGSTLAYSDR LSMASGSIGV GVLGVQNAGP
GSSVVTQLTA QQLGMMGMEA LDTVINFWED ALAAHYSPGG LPALLTTAED SEFCREIQNL
LEMAYTLQEQ SELLFLDQRS VLFREEHSID EAEEEAGEAD DDRRSRKSGS VLSRAGSDPN
FDSAESFASA LDQVADLREF DGFIETSYEE YPLFQSALKH HDEYTVPCRT IRAELMHCST
DTEYLAKLHC VRLAFQFLFK DPAVGQWICD AGRQILTDLL CLGDKDTKEF LVGYEDMVNY
LHDSNNWPCI QMELEQRNVK AMTFYDICLD FIILDSFKDL DAPPASVTAV VQNRWLSNGF
KETALTTAVW SVLKAKKRML KFPNGFMSHF YVISEQISPL MAWGFFGPNE NLRDICHYFR
EELLAFLGDI FSFQKSRFTT IEEFSQDVLQ HMQTRVNNIG VKFSQ
