MILR1_HUMAN
ID MILR1_HUMAN Reviewed; 343 AA.
AC Q7Z6M3; A1L458; B4DUS1; B7ZM50; B9EGN0; D6RUW6; D6RUW8;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Allergin-1;
DE AltName: Full=Allergy inhibitory receptor 1;
DE AltName: Full=Mast cell antigen 32;
DE Short=MCA-32;
DE AltName: Full=Mast cell immunoglobulin-like receptor 1;
DE Flags: Precursor;
GN Name=MILR1; Synonyms=C17orf60, MCA32;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=20526344; DOI=10.1038/ni.1886;
RA Hitomi K., Tahara-Hanaoka S., Someya S., Fujiki A., Tada H., Sugiyama T.,
RA Shibayama S., Shibuya K., Shibuya A.;
RT "An immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E-
RT mediated immediate hypersensitivity reactions.";
RL Nat. Immunol. 11:601-607(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Prostate, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Immunoglobulin-like receptor which plays an inhibitory role
CC in degranulation of mast cells. Negatively regulates IgE-mediated mast
CC cell activation and suppresses the type I immediate hypersensitivity
CC reaction (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Monomer. Interacts (tyrosine-phosphorylated) with PTPN6,
CC PTPN11 and INPP5D. {ECO:0000250}.
CC -!- INTERACTION:
CC Q7Z6M3; Q6UX41-6: BTNL8; NbExp=3; IntAct=EBI-18391669, EBI-17442596;
CC Q7Z6M3; Q8WVX3-2: C4orf3; NbExp=3; IntAct=EBI-18391669, EBI-12003442;
CC Q7Z6M3; Q96F15: GIMAP5; NbExp=3; IntAct=EBI-18391669, EBI-6166686;
CC Q7Z6M3; Q9HCP6: HHATL; NbExp=3; IntAct=EBI-18391669, EBI-5916693;
CC Q7Z6M3; O75425: MOSPD3; NbExp=3; IntAct=EBI-18391669, EBI-12179105;
CC Q7Z6M3; Q9NV12: TMEM140; NbExp=3; IntAct=EBI-18391669, EBI-2844246;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20526344};
CC Single-pass type I membrane protein {ECO:0000269|PubMed:20526344}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=L;
CC IsoId=Q7Z6M3-1; Sequence=Displayed;
CC Name=2; Synonyms=S1;
CC IsoId=Q7Z6M3-2; Sequence=VSP_040766;
CC Name=3; Synonyms=S2;
CC IsoId=Q7Z6M3-3; Sequence=VSP_040765;
CC -!- TISSUE SPECIFICITY: Expressed in myeloid cells (dendritic cells,
CC macrophages and neutrophils, weak expression on B-cells but not in T-
CC cells or natural killer cells), peripheral blood basophils and mast
CC cells (at protein level). {ECO:0000269|PubMed:20526344}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:20526344}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI36595.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG62433.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB542950; BAJ08251.1; -; mRNA.
DR EMBL; AB542951; BAJ08252.1; -; mRNA.
DR EMBL; AB542952; BAJ08253.1; -; mRNA.
DR EMBL; AK300768; BAG62433.1; ALT_INIT; mRNA.
DR EMBL; BC053534; AAH53534.1; -; mRNA.
DR EMBL; BC130406; AAI30407.2; -; mRNA.
DR EMBL; BC136594; AAI36595.1; ALT_INIT; mRNA.
DR EMBL; BC144272; AAI44273.1; -; mRNA.
DR CCDS; CCDS74133.1; -. [Q7Z6M3-1]
DR CCDS; CCDS77087.1; -. [Q7Z6M3-2]
DR CCDS; CCDS77088.1; -. [Q7Z6M3-3]
DR RefSeq; NP_001078892.1; NM_001085423.1. [Q7Z6M3-1]
DR RefSeq; NP_001278245.1; NM_001291316.1. [Q7Z6M3-2]
DR RefSeq; NP_001278246.1; NM_001291317.1. [Q7Z6M3-3]
DR RefSeq; XP_016879975.1; XM_017024486.1.
DR AlphaFoldDB; Q7Z6M3; -.
DR BioGRID; 129734; 76.
DR IntAct; Q7Z6M3; 13.
DR STRING; 9606.ENSP00000482801; -.
DR GlyGen; Q7Z6M3; 6 sites.
DR iPTMnet; Q7Z6M3; -.
DR PhosphoSitePlus; Q7Z6M3; -.
DR BioMuta; MILR1; -.
DR DMDM; 160013631; -.
DR EPD; Q7Z6M3; -.
DR MassIVE; Q7Z6M3; -.
DR MaxQB; Q7Z6M3; -.
DR PeptideAtlas; Q7Z6M3; -.
DR PRIDE; Q7Z6M3; -.
DR ProteomicsDB; 69447; -. [Q7Z6M3-1]
DR ProteomicsDB; 69448; -. [Q7Z6M3-2]
DR ProteomicsDB; 69449; -. [Q7Z6M3-3]
DR Antibodypedia; 68852; 110 antibodies from 14 providers.
DR DNASU; 284021; -.
DR Ensembl; ENST00000612535.4; ENSP00000477504.1; ENSG00000271605.6. [Q7Z6M3-3]
DR Ensembl; ENST00000615220.4; ENSP00000480749.1; ENSG00000271605.6. [Q7Z6M3-1]
DR Ensembl; ENST00000616498.4; ENSP00000481318.1; ENSG00000271605.6. [Q7Z6M3-2]
DR Ensembl; ENST00000619286.5; ENSP00000482801.1; ENSG00000271605.6. [Q7Z6M3-1]
DR GeneID; 284021; -.
DR KEGG; hsa:284021; -.
DR MANE-Select; ENST00000619286.5; ENSP00000482801.1; NM_001085423.2; NP_001078892.1.
DR UCSC; uc010wpz.4; human. [Q7Z6M3-1]
DR CTD; 284021; -.
DR DisGeNET; 284021; -.
DR GeneCards; MILR1; -.
DR HGNC; HGNC:27570; MILR1.
DR HPA; ENSG00000271605; Tissue enhanced (lymphoid).
DR MalaCards; MILR1; -.
DR neXtProt; NX_Q7Z6M3; -.
DR OpenTargets; ENSG00000271605; -.
DR PharmGKB; PA142672243; -.
DR VEuPathDB; HostDB:ENSG00000271605; -.
DR eggNOG; ENOG502S5BW; Eukaryota.
DR GeneTree; ENSGT01050000244988; -.
DR HOGENOM; CLU_069833_0_0_1; -.
DR InParanoid; Q7Z6M3; -.
DR OMA; FPNNTKY; -.
DR OrthoDB; 1405395at2759; -.
DR PhylomeDB; Q7Z6M3; -.
DR PathwayCommons; Q7Z6M3; -.
DR SignaLink; Q7Z6M3; -.
DR BioGRID-ORCS; 284021; 6 hits in 226 CRISPR screens.
DR ChiTaRS; MILR1; human.
DR GenomeRNAi; 284021; -.
DR Pharos; Q7Z6M3; Tbio.
DR PRO; PR:Q7Z6M3; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q7Z6M3; protein.
DR Bgee; ENSG00000271605; Expressed in monocyte and 121 other tissues.
DR ExpressionAtlas; Q7Z6M3; baseline and differential.
DR Genevisible; Q7Z6M3; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IBA:GO_Central.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0098742; P:cell-cell adhesion via plasma-membrane adhesion molecules; IBA:GO_Central.
DR GO; GO:0043303; P:mast cell degranulation; ISS:UniProtKB.
DR GO; GO:0033004; P:negative regulation of mast cell activation; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR029626; Allergin-1.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR040878; Ig_C17orf99.
DR PANTHER; PTHR11481:SF58; PTHR11481:SF58; 1.
DR Pfam; PF17736; Ig_C17orf99; 2.
DR SUPFAM; SSF48726; SSF48726; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome;
KW Repeat; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..343
FT /note="Allergin-1"
FT /id="PRO_0000307364"
FT TOPO_DOM 20..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 35..118
FT /note="Ig-like C2-type 1"
FT DOMAIN 128..213
FT /note="Ig-like C2-type 2"
FT MOTIF 311..316
FT /note="ITIM motif"
FT MOTIF 336..341
FT /note="ITIM motif"
FT MOD_RES 313
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3TB92"
FT MOD_RES 338
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3TB92"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 56..103
FT /evidence="ECO:0000250"
FT DISULFID 147..196
FT /evidence="ECO:0000250"
FT VAR_SEQ 33..122
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:20526344"
FT /id="VSP_040765"
FT VAR_SEQ 123..217
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:20526344"
FT /id="VSP_040766"
SQ SEQUENCE 343 AA; 38735 MW; DCD1E784D40885C5 CRC64;
MWSHLNRLLF WSIFSSVTCR KAVLDCEAMK TNEFPSPCLD SKTKVVMKGQ NVSMFCSHKN
KSLQITYSLF RRKTHLGTQD GKGEPAIFNL SITEAHESGP YKCKAQVTSC SKYSRDFSFT
IVDPVTSPVL NIMVIQTETD RHITLHCLSV NGSLPINYTF FENHVAISPA ISKYDREPAE
FNLTKKNPGE EEEYRCEAKN RLPNYATYSH PVTMPSTGGD SCPFCLKLLL PGLLLLLVVI
ILILAFWVLP KYKTRKAMRN NVPRDRGDTA MEVGIYANIL EKQAKEESVP EVGSRPCVST
AQDEAKHSQE LQYATPVFQE VAPREQEACD SYKSGYVYSE LNF
