MIM1_YEAST
ID MIM1_YEAST Reviewed; 113 AA.
AC Q08176; D6W239;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Mitochondrial import protein 1;
DE AltName: Full=Mitochondrial 13 kDa outer membrane protein;
GN Name=MIM1; Synonyms=TOM13; OrderedLocusNames=YOL026C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15326197; DOI=10.1083/jcb.200405138;
RA Ishikawa D., Yamamoto H., Tamura Y., Moritoh K., Endo T.;
RT "Two novel proteins in the mitochondrial outer membrane mediate beta-barrel
RT protein assembly.";
RL J. Cell Biol. 166:621-627(2004).
RN [5]
RP FUNCTION.
RX PubMed=15242642; DOI=10.1016/j.cell.2004.06.013;
RA Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W.,
RA Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D.,
RA Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N.,
RA Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J.,
RA Hughes T.R.;
RT "Exploration of essential gene functions via titratable promoter alleles.";
RL Cell 118:31-44(2004).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=15608614; DOI=10.1038/sj.embor.7400318;
RA Waizenegger T., Schmitt S., Zivkovic J., Neupert W., Rapaport D.;
RT "Mim1, a protein required for the assembly of the TOM complex of
RT mitochondria.";
RL EMBO Rep. 6:57-62(2005).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17974559; DOI=10.1074/jbc.m706997200;
RA Becker T., Pfannschmidt S., Guiard B., Stojanovski D., Milenkovic D.,
RA Kutik S., Pfanner N., Meisinger C., Wiedemann N.;
RT "Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and
RT assembly of signal-anchored receptors.";
RL J. Biol. Chem. 283:120-127(2008).
RN [8]
RP FUNCTION, AND SUBUNIT.
RX PubMed=18187149; DOI=10.1016/j.jmb.2007.12.021;
RA Hulett J.M., Lueder F., Chan N.C., Perry A.J., Wolynec P., Likic V.A.,
RA Gooley P.R., Lithgow T.;
RT "The transmembrane segment of Tom20 is recognized by Mim1 for docking to
RT the mitochondrial TOM complex.";
RL J. Mol. Biol. 376:694-704(2008).
RN [9]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH TOM70.
RX PubMed=21825073; DOI=10.1083/jcb.201102044;
RA Becker T., Wenz L.S., Krueger V., Lehmann W., Mueller J.M., Goroncy L.,
RA Zufall N., Lithgow T., Guiard B., Chacinska A., Wagner R., Meisinger C.,
RA Pfanner N.;
RT "The mitochondrial import protein Mim1 promotes biogenesis of multispanning
RT outer membrane proteins.";
RL J. Cell Biol. 194:387-395(2011).
RN [10]
RP FUNCTION.
RX PubMed=21825074; DOI=10.1083/jcb.201102041;
RA Papic D., Krumpe K., Dukanovic J., Dimmer K.S., Rapaport D.;
RT "Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-
RT dependent import pathway.";
RL J. Cell Biol. 194:397-405(2011).
RN [11]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH MIM2.
RX PubMed=22467864; DOI=10.1242/jcs.103804;
RA Dimmer K.S., Papic D., Schumann B., Sperl D., Krumpe K., Walther D.M.,
RA Rapaport D.;
RT "A crucial role for Mim2 in the biogenesis of mitochondrial outer membrane
RT proteins.";
RL J. Cell Sci. 125:3464-3473(2012).
CC -!- FUNCTION: Component of the MIM complex required for mitochondrial outer
CC membrane protein import (PubMed:22467864). The MIM complex cooperates
CC with the receptor TOM70 in binding of precursor proteins and promotes
CC their insertion and assembly into the outer membrane (PubMed:21825073).
CC Involved in import of the subset of proteins with multiple alpha-
CC helical transmembrane segments, including UGO1 (PubMed:21825073,
CC PubMed:21825074). Required for the assembly of the TOM (translocase of
CC outer membrane) receptor complex, which is responsible for the
CC recognition and translocation of cytosolically synthesized
CC mitochondrial preproteins (PubMed:15242642, PubMed:15326197,
CC PubMed:15608614). Required specifically for assembly of TOM40, TOM20,
CC and TOM70, but not TOM22 (PubMed:17974559, PubMed:18187149).
CC {ECO:0000269|PubMed:15242642, ECO:0000269|PubMed:15326197,
CC ECO:0000269|PubMed:15608614, ECO:0000269|PubMed:17974559,
CC ECO:0000269|PubMed:18187149, ECO:0000269|PubMed:21825073,
CC ECO:0000269|PubMed:21825074, ECO:0000269|PubMed:22467864}.
CC -!- SUBUNIT: Component of the MIM complex containing at least MIM1 and MIM2
CC (PubMed:15608614, PubMed:18187149, PubMed:21825073, PubMed:22467864).
CC Interacts with MIM2; interaction is direct (PubMed:22467864). Interacts
CC with TOM70 (PubMed:21825073). {ECO:0000269|PubMed:15608614,
CC ECO:0000269|PubMed:18187149, ECO:0000269|PubMed:21825073,
CC ECO:0000269|PubMed:22467864}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:15326197, ECO:0000269|PubMed:15608614}.
CC -!- DISRUPTION PHENOTYPE: Leads to reduced levels of TOM complex but
CC retains the respiratory chain. {ECO:0000269|PubMed:17974559}.
CC -!- SIMILARITY: Belongs to the MIM1 family. {ECO:0000305}.
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DR EMBL; Z74768; CAA99026.1; -; Genomic_DNA.
DR EMBL; AY557996; AAS56322.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10755.1; -; Genomic_DNA.
DR PIR; S66709; S66709.
DR RefSeq; NP_014616.1; NM_001183280.1.
DR AlphaFoldDB; Q08176; -.
DR BioGRID; 34374; 25.
DR IntAct; Q08176; 4.
DR MINT; Q08176; -.
DR STRING; 4932.YOL026C; -.
DR TCDB; 1.B.33.3.1; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR iPTMnet; Q08176; -.
DR PaxDb; Q08176; -.
DR PRIDE; Q08176; -.
DR EnsemblFungi; YOL026C_mRNA; YOL026C; YOL026C.
DR GeneID; 854131; -.
DR KEGG; sce:YOL026C; -.
DR SGD; S000005386; MIM1.
DR VEuPathDB; FungiDB:YOL026C; -.
DR eggNOG; ENOG502SBHA; Eukaryota.
DR HOGENOM; CLU_2134979_0_0_1; -.
DR InParanoid; Q08176; -.
DR BioCyc; YEAST:G3O-33442-MON; -.
DR PRO; PR:Q08176; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08176; protein.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0070096; P:mitochondrial outer membrane translocase complex assembly; IMP:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IMP:SGD.
DR InterPro; IPR013262; OMP_MIM1/TOM13_mt.
DR PANTHER; PTHR28241; PTHR28241; 1.
DR Pfam; PF08219; TOM13; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..113
FT /note="Mitochondrial import protein 1"
FT /id="PRO_0000218767"
FT TRANSMEM 39..62
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 113 AA; 12758 MW; 3A3064F6D00BA35B CRC64;
MTEVVGFWES VSDDESEDKD CMEVQNTVSA DESPLVQSLV SFVGSCSINL LLPFLNGMML
GFGELFAHEL CWRFNWFNHR NKGYKVYPES RKIAALKEIS SPGTRGRVAS KFL
